High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses

Abstract

Background: Owing to the growing interest in biofuels, the concept of a biorefinery where biomass is converted to a variety of useful products is gaining ground. We here present how distillery waste is combined with a by-product from a sugar production, molasses, to form a medium for the growth of Lactobacillus plantarum with yields and biomass densities comparable with conventional industrial media. Such approach enables a cost-effective utilization of the problematic wastewater from ethanol and a by-product from sugar production. It is the first approach that attempts to find low-cost media for the production of Lactobacillus plantarum biomass. Results: This study suggests that sieved wheat stillage enriched by adding 1.77 g/l yeast extract and 10% molasses (v/v), with NH4OH used for pH adjustment, may be used as a media for large-scale cultivation of L. plantarum. Such composition of the medium permits a high density of lactic acid bacteria (1.6 x 10 10 cfu/ml) to be achieved. Conclusions: The use of a fermentation medium consisting of distillery wastewater and molasses to obtain value-added products (such as LAB biomass and lactic acid) is a possible step for classical ethanol production to move towards a biorefinery model production in which all by and waste products are utilized to increase produced values and reduce waste production. This enables a cost-effective utilization of the problematic wastewater from ethanol and sugar production.

Sweet Potatoes as a Basic Component in Developing a Medium for the Cultivation of Lactobacilli

“…13) For high density production of lactobacilli using wheat stillage and molasses, the medium was supplemented with yeast extract. 26) Thus, supplementation of SPM with peptone, yeast extract, and beef extract is required to ensure the normal growth of lactobacilli.…”

Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938

“…Beet molasses as a sole fermentation medium generally leads to low biomass growth, due to its high salt concentration and pH, and the presence of betaine as the main nitrogen source [4]. In combination with wheat stillage as nitrogen sources, however, adding beet molasses led to higher bacterial biomass [13]. The authors attributed the increase of LAB biomass to the rise of sugar concentration.…”

“…MRS is composed of ten different ingredients among others polypeptones and meat and yeast extracts, which are quite expensive. Pertinent studies have been conducted to develop less expensive media supporting the growth of particular species of lactobacillus, such as Lactobacillus casei [12], Lactobacillus delbrueckii [5,6], or Lactobacillus plantarum [13] but, to the best of our knowledge, such a study involving L. reuteri is lacking.…”

Use of Lactobacillus for Improved Growth and Enhanced Biochemical, Hematological, and Digestive Enzyme Activity in Common Carp at Mazandaran, Iran

“…). This probiotic has been studied by several authors that used agricultural wastes, such as coffee husk, tamarind seed powder, bagasse, ground nut oil cake, wheat bran, and rice bran and solid substrates (Natarajan and Rajendran ); sugar beet molasses (Krzywonos and Eberhard ); and quinoa (Dallagnol et al. ) for L. plantarum biomass production and for its metabolites of interest, particularly lactic acid.…”