Sweet Potatoes as a Basic Component in Developing a Medium for the Cultivation of Lactobacilli

Hayek, Saeed A.; Shahbazi, Aboghasem; Awaisheh, Saddam S.; Shah, Nagendra P.; Ibrahim, Salam A.

doi:10.1271/bbb.130508

Cited by 20 publications

(19 citation statements)

References 19 publications

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“…Earlier results also showed that date palm extract supplemented with external nitrogen sources can support lactobacilli growth (Nancib et al, 2001). Our results also agree with the work of Hayak et al (Hayek et al, 2013) who observed that L. reuteri grew well in sweet potato based medium supplemented with a nitrogen source. Date palm is a rich source of a variety of nutrients and has the potential to either partly or fully replace the expensive ingredients of LAB media.…”

Section: Discussionsupporting

confidence: 93%

“…Many attempts have been made to use agricultural by-products to substitute the standard sources of nitrogen at a lower cost. Good examples are the use of hydrolysates of wheat bran mixed with corn steep liquor (Li, Han, Ji, Wang, & Tan, 2010), corn steep liquor supplemented with Tween 80, and K 2 HPO 4 (Coelho, Augusto, & Lages, 2011), cane molasses mixed with animal and marine by-products (Khannous et al, 2003), and sweet potato supplemented with yeast extract (Hayek et al, 2013). We believe that dates are a good substitute source of carbon, nitrogen, vitamins, and minerals and could be used in a low-cost media for the production of LAB.…”

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confidence: 99%

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Using Date Palm (Phoenix dactylifera L.) by-products to Cultivate Lactobacillus reuteri spp.

Ayad

El-Rab

Shahbazi

et al. 2016

JFR

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Lactic acid bacteria (LAB) are used by industry to produce fermented food products. The standard media used to cultivate LABs is DeMan Rogosa Sharp (MRS). However, it is expensive. Alternative low-cost media must be developed for industrial use. A good source for growth media components are by-products generated during the production of agricultural goods, such as dates. Our objective was to investigate the use of date by-products for cultivating Lactobacillus reuteri.Date palm extract (DPE) was prepared by pressing fresh date fruits for one week and diluting 1:2 with diH 2 O, centrifuging at 4696 x g and 4°C 25 min, and autoclaving the supernatant at 110°C for 15 min. An MRS-based buffer solution was added to DPE make a date palm medium (DPM). DPM was then enriched with various amounts phytone peptone (0, 0.2, 0.4, 0.6, and 0.8 %, w/v). The enriched DPMs were used to cultivate three strains of Lactobacillus reuteri: DSM 20016, CF2-7F, and SD 2112. Our results showed that in the DPM minus phytone peptone, bacterial counts reached 3.18 ± 0.5 log CFU/mL. Addition of lower amounts of phytone peptone did not improve bacterial growth. However, DPM medium supplemented with 0.8% phytone peptone improved the bacterial counts, which reached 6.94 ± 0.1 log CFU/mL, similar to what was observed with MRS (7.90± 0.24 log CFU/mL). There was no significant difference in the growth of LAB in MRS and phytone peptone enriched media DPM (p > 0.05). Date by-products are potentially alternative low cost components of LAB growth media.

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

Using Date Palm (Phoenix dactylifera L.) by-products to Cultivate Lactobacillus reuteri spp.

Ayad

El-Rab

Shahbazi

et al. 2016

JFR

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“…Even though protein sources such as yeast and beef extract showed higher buffering capacities and high cell numbers, these ingredients are expensive and comprise a significant portion of media costs [18]. It has been estimated that yeast extract contributes >30% of the total production cost due to its high price [23].…”

Section: Discussionmentioning

confidence: 99%

“…Maximum specific growth rates per hour for the tested strains were determined during the exponential growth phase by measuring optical density values [18]. …”

Section: Methodsmentioning

confidence: 99%

Use of Phytone Peptone to Optimize Growth and Cell Density of Lactobacillus reuteri

Atilola

Gyawali

Aljaloud

et al. 2015

Foods

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The objective of this study was to determine the use of phytone peptone to optimize the growth and cell density of Lactobacillus reuteri. Four strains of L. reuteri (DSM 20016, SD 2112, CF 2-7F, and MF 2-3,) were used in this study. An overnight culture of individual strains was inoculated into fresh basal media with various protein sources (peptone, tryptone, proteose peptone #3, phytone peptone, tryptic soy broth, yeast extract, and beef extract). Samples were then mixed well and incubated at 37 °C for 15 h. Bacterial growth was monitored by measuring turbidity (optical density 610 nm) at different time intervals during the incubation period. At the end of incubation, samples were plated on de-Man Rogosa Sharpe (MRS) agar to determine the bacterial population. Our results showed that phytone peptone promoted the growth of L. reuteri (p < 0.05) by 1.4 log CFU/mL on average compared to the control samples. Therefore, phytone peptone could be included in laboratory media to enhance growth and increase the cell density of L. reuteri.

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“…Previous studies have shown that plant components can support the growth and functionality of probiotic bacteria (Gyawali and Ibrahim 2012). We have previously showed that sweet potatoes could be used to form an alternative low cost medium for the growth of Lactobacillus strains (Hayek et al 2013). Lactobacillus strains grown in a sweet potato base medium were also found to produce higher β -glucosidase, acid phosphatase, and phytase activities and lower α –glucosidase than that in MRS (Hayek 2013).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic activity of Lactobacillus reuteri grown in a sweet potato based medium with the addition of metal ions

et al. 2013

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The effect of metal ions on the enzymatic activity of Lactobacillus reuteri was studied. The enzymatic activity was determined spectrophotometrically using the corresponding substrate. In the control group, L. reuteri MF14-C, MM2-3, SD2112, and DSM20016 produced the highest α-glucosidase (40.06 ± 2.80 Glu U/mL), β-glucosidase (17.82 ± 1.45 Glu U/mL), acid phosphatase (20.55 ± 0.74 Ph U/mL), and phytase (0.90 ± 0.05 Ph U/mL) respectively. The addition of Mg2+ and Mn2+ led to enhance α-glucosidase produced by L. reuteri MM2-3 by 113.6% and 100.6% respectively. α-Glucosidase produced by MF14-C and CF2-7F was decrease in the presence of K+ by 65.8 and 69.4% respectively. β-Glucosidase activity of MM7 and SD2112 increased in the presence of Ca2+ (by 121.8 and 129.8%) and Fe2+ (by 143.9 and 126.7%) respectively. Acid phosphatase produced by L. reuteri CF2-7F and MM2-3 was enhanced in the presence of Mg2+, Ca2+ or Mn2+ by (94.7, 43.2, and 70.1%) and (63.1, 67.8, and 45.6%) respectively. On the other hand, Fe2+, K+, and Na+ caused only slight increase or decrease in acid phosphatase activity. Phytase produced by L. reuteri MM2-3 was increase in the presence of Mg2+ and Mn2+ by 51.0 and 74.5% respectively. Ca2+ enhanced phytase activity of MM2-3 and DSM20016 by 27.5 and 28.9% respectively. The addition of Na+ or Fe2+ decreased phytase activity of L. reuteri. On average, Mg2+ and Mn2+ followed by Ca2+ led to the highest enhancement of the tested enzymes. However, the effect of each metal ion on the enzymatic activity of L. reuteri was found to be a strain dependent. Therefore, a maximized level of a target enzyme could be achieved by selecting a combination of specific strain and specific metal ion.

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Sweet Potatoes as a Basic Component in Developing a Medium for the Cultivation of Lactobacilli

Cited by 20 publications

References 19 publications

Using Date Palm (Phoenix dactylifera L.) by-products to Cultivate Lactobacillus reuteri spp.

Using Date Palm (Phoenix dactylifera L.) by-products to Cultivate Lactobacillus reuteri spp.

Use of Phytone Peptone to Optimize Growth and Cell Density of Lactobacillus reuteri

Enzymatic activity of Lactobacillus reuteri grown in a sweet potato based medium with the addition of metal ions

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