Use of Phytone Peptone to Optimize Growth and Cell Density of Lactobacillus reuteri

Atilola, Olabiyi Akinbami; Gyawali, Rabin; Aljaloud, Sulaiman O.; Ibrahim, Salam A.

doi:10.3390/foods4030318

Cited by 14 publications

(22 citation statements)

References 22 publications

(24 reference statements)

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“…Previously, we found that phytone peptone (PP) is the most important growth-promoting factor for Lactobacillus reuteri (Atilola, Gyawali, Aljaloud, & Ibrahim, 2015). Our results indicate that DPM supplemented with PP support the growth of L. reuteri.…”

Section: Discussionsupporting

confidence: 54%

Using Date Palm (Phoenix dactylifera L.) by-products to Cultivate Lactobacillus reuteri spp.

Ayad

El-Rab

Shahbazi

et al. 2016

JFR

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Lactic acid bacteria (LAB) are used by industry to produce fermented food products. The standard media used to cultivate LABs is DeMan Rogosa Sharp (MRS). However, it is expensive. Alternative low-cost media must be developed for industrial use. A good source for growth media components are by-products generated during the production of agricultural goods, such as dates. Our objective was to investigate the use of date by-products for cultivating Lactobacillus reuteri.Date palm extract (DPE) was prepared by pressing fresh date fruits for one week and diluting 1:2 with diH 2 O, centrifuging at 4696 x g and 4°C 25 min, and autoclaving the supernatant at 110°C for 15 min. An MRS-based buffer solution was added to DPE make a date palm medium (DPM). DPM was then enriched with various amounts phytone peptone (0, 0.2, 0.4, 0.6, and 0.8 %, w/v). The enriched DPMs were used to cultivate three strains of Lactobacillus reuteri: DSM 20016, CF2-7F, and SD 2112. Our results showed that in the DPM minus phytone peptone, bacterial counts reached 3.18 ± 0.5 log CFU/mL. Addition of lower amounts of phytone peptone did not improve bacterial growth. However, DPM medium supplemented with 0.8% phytone peptone improved the bacterial counts, which reached 6.94 ± 0.1 log CFU/mL, similar to what was observed with MRS (7.90± 0.24 log CFU/mL). There was no significant difference in the growth of LAB in MRS and phytone peptone enriched media DPM (p > 0.05). Date by-products are potentially alternative low cost components of LAB growth media.

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Section: Discussionsupporting

confidence: 54%

Using Date Palm (Phoenix dactylifera L.) by-products to Cultivate Lactobacillus reuteri spp.

Ayad

El-Rab

Shahbazi

et al. 2016

JFR

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“…rhamnosus E/N [ 33 ] and L . reuteri [ 38 ]. Hebert et al [ 39 ] showed that Tween 80 had different effects on the growth of several LAB: it has stimulated L .…”

Section: Discussionmentioning

confidence: 99%

Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098

et al. 2018

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We have demonstrated previously that a soluble factor (LrS) produced by Lactobacillus (L.) reuteri CRL 1098 modulates the inflammatory response triggered by lipopolysaccharide. In this study, the production of LrS by L. reuteri CRL 1098 was realized through two steps: i) bacterial biomass production, ii) LrS production, where the bacterial biomass was able to live but did not proliferate. Therefore, the simultaneous evaluation of the effect of different factors on the growth and LrS production was performed. Biomass production was found to be dependent mainly on culture medium, while LrS production with anti-inflammatory activity depended on culture conditions of the biomass such as pH, agitation and growth phase. The L. reuteri CRL 1098 biomass and LrS production in the optimized culture media designed for this work reduced the complete process cost by approximately 95%, respectively to laboratory scale cost.

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“…This ingredient was developed as an alternative nitrogen source in dehydrated culture media to accommodate various nutritional requirements of LAB. Other non-animal protein sources such as phytone peptone have also been shown to support the growth of LAB (Atilola et al ., 2015). Phytone peptone is an ultra-filtered enzymatic digest of soybean meal and has a high buffering capacity that helps to maintain the pH level during the fermentation process.…”

Section: Standard Mediamentioning

confidence: 99%

“…This protein has been shown to enhance growth and increase the cell mass of Lb. reuteri (Atilola et al ., 2015). The primary ingredient in M17 is tryptone peptone which is a pancreatic digest of casein that is recommended for the rapid growth and detection of microorganisms present in low concentrations.…”

Section: Standard Mediamentioning

confidence: 99%

Cultivation media for lactic acid bacteria used in dairy products

Hayek

Gyawali

Aljaloud

et al. 2019

Journal of Dairy Research

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This review aims to familiarize the reader with research efforts on the cultivation media of lactic acid bacteria (LAB). We have also included a brief discussion on standard ingredients used in LAB media and chemically defined media as related to bacterial growth requirements. Recent research has focused on modifying standard media for the enumeration, differentiation, isolation, and identification of starter cultures and probiotics. Even though large numbers of these media have been developed to serve dairy microbial control, they have failed to provide consistent results. The research consequently points to the need to develop a reliable lactobacilli growth medium for the dairy industry.

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Use of Phytone Peptone to Optimize Growth and Cell Density of Lactobacillus reuteri

Cited by 14 publications

References 22 publications

Using Date Palm (Phoenix dactylifera L.) by-products to Cultivate Lactobacillus reuteri spp.

Using Date Palm (Phoenix dactylifera L.) by-products to Cultivate Lactobacillus reuteri spp.

Two-step production of anti-inflammatory soluble factor by Lactobacillus reuteri CRL 1098

Cultivation media for lactic acid bacteria used in dairy products

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