High-density collagen gel tubes as a matrix for primary human bladder smooth muscle cells

Micol, Lionel A.; Ananta, M.; Engelhardt, Eva; Mudera, Vivek; Brown, Robert A.; Hubbell, Jeffrey A.; Frey, Peter

doi:10.1016/j.biomaterials.2010.10.028

Cited by 50 publications

(41 citation statements)

References 24 publications

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“…To date, four distinct methods have been reported to modulate CFD: reverse dialysis [6], evaporation [7], plastic compression (PC) [8], and continuous injection [9]. These techniques allow for the fabrication of dense collagen (DC) gels with a fibrillar density within the range of 5e25 wt%, which have been used as 3D ECM models [10,11] and to engineer several tissues, such as osteoid [12e14], dura mater [15], nucleus pulpous [16,17], skin [18,19], bladder [20], cornea [21e24], tendon [25], airways [26], and blood vessels [26]. Nevertheless, none of these methods have allowed for the formation of anisotropic DC gels [3], a feature that has been achieved in highlyhydrated collagen gels (fibrillar density < 1 wt%) through the application of either electric and magnetic fields [27e29], microfluidic devices [30,31], extrusion of crosslinked collagen [32], micro-and nano-topographical cues during self-assembly [33,34], or strain during fibril formation [35].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of injectable, cellular, anisotropic collagen tissue equivalents with modular fibrillar densities

Marelli¹,

Ghezzi²,

James-Bhasin³

et al. 2015

Biomaterials

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Section: Introductionmentioning

confidence: 99%

Fabrication of injectable, cellular, anisotropic collagen tissue equivalents with modular fibrillar densities

Marelli¹,

Ghezzi²,

James-Bhasin³

et al. 2015

Biomaterials

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“…Collagen as a polymer has been fabricated and used as a biomaterial for various tissue-engineering applications 36,37 and also for tendon tissue engineering. 31,38–40 Collagen exhibits all characteristics of an ideal biomaterial for tendon TE described by Liu et al 41 and by Henson and Getgood, 42 but the mechanical properties of TE collagen construct are currently inferior to the intact tendon.…”

Section: Discussionmentioning

confidence: 99%

Development of a Surgically Optimized Graft Insertion Suture Technique to Accommodate a Tissue-Engineered TendonIn Vivo

Sawadkar

Alexander

Tolk

et al. 2013

BioResearch Open Access

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The traumatic rupture of tendons is a common clinical problem. Tendon repair is surgically challenging because the tendon often retracts, resulting in a gap between the torn end and its bony insertion. Tendon grafts are currently used to fill this deficit but are associated with potential complications relating to donor site morbidity and graft necrosis. We have developed a highly reproducible, rapid process technique to manufacture compressed cell-seeded type I collagen constructs to replace tendon grafts. However, the material properties of the engineered constructs are currently unsuitable to withstand complete load bearing in vivo. A modified suture technique has been developed to withstand physiological loading and off load the artificial construct while integration occurs. Lapine tendons were used ex vivo to test the strength of different suture techniques with different sizes of Prolene sutures and tissue-engineered collagen constructs in situ. The data were compared to standard modified Kessler suture using a standard tendon graft. Mechanical testing was carried out and a finite element analysis stress distribution model constructed using COMSOL 3.5 software. The break point for modified suture technique with a tissue-engineered scaffold was significantly higher (50.62 N) compared to a standard modified Kessler suture (12.49 N, p<0.05). Distributing suture tension further proximally and distally from the tendon ends increased the mechanical strength of the repairs. We now have ex vivo proof of concept that this suture technique is suitable for testing in vivo, and this will be the next stage of our research.

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“…Some instability issues can be counteracted with a higher concentration of polymer in the gel, but attention has to be paid to the resulting pore size. Higher concentrated gels form a dense network which impedes cell migration and ingrowth into the matrix [23,24]. Lower concentrated gels have poor mechanical properties and may tear easily during implantation.…”

Section: Modifications For Enhanced Stabilitymentioning

confidence: 99%

Hydrogels based on collagen and fibrin – frontiers and applications

Schneider-Barthold¹,

Baganz²,

Wilhelmi

et al. 2016

BioNanoMaterials

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Hydrogels are a versatile tool for a multitude of applications in biomedical research and clinical practice. Especially collagen and fibrin hydrogels are distinguished by their excellent biocompatibility, natural capacity for cell adhesion and low immunogenicity. In many ways, collagen and fibrin represent an ideal biomaterial, as they can serve as a scaffold for tissue regeneration and promote the migration of cells, as well as the ingrowth of tissues. On the other hand, pure collagen and fibrin materials are marked by poor mechanical properties and rapid degradation, which limits their use in practice. This paper will review methods of modification of natural collagen and fibrin materials to next-generation materials with enhanced stability. A special focus is placed on biomedical products from fibrin and collagen already on the market. In addition, recent research on the in vivo applications of collagen and fibrin-based materials will be showcased.

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High-density collagen gel tubes as a matrix for primary human bladder smooth muscle cells

Cited by 50 publications

References 24 publications

Fabrication of injectable, cellular, anisotropic collagen tissue equivalents with modular fibrillar densities

Fabrication of injectable, cellular, anisotropic collagen tissue equivalents with modular fibrillar densities

Development of a Surgically Optimized Graft Insertion Suture Technique to Accommodate a Tissue-Engineered TendonIn Vivo

Hydrogels based on collagen and fibrin – frontiers and applications

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