High cell density cultivation of Lipomyces starkeyi for achieving highly efficient lipid production from sugar under low C/N ratio

Amza, Rezky Lastinov; Kahar, Prihardi; Juanssilfero, Ario Betha; Miyamoto, Nao; Otsuka, Hiromi; Kondo, Akihiko

doi:10.1016/j.bej.2019.05.013

Cited by 19 publications

(8 citation statements)

References 30 publications

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“…The remaining fatty acids included stearic acid (C18:0) with a content of 4.59–5.97%, palmitoleic acid (C16:1) with a content of 3.01–3.96%, and linoleic acid (C18:2) with a content of 1.12–2.93%, respectively. Such a fatty acids profile, similar to the main components of vegetable oil, was consistent with several previous studies [ 42 , 43 , 44 ]. The differences in fatty acids profiles among different fermentation flasks were analysed by significance analysis.…”

Section: Resultssupporting

confidence: 92%

Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel

et al. 2021

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The high operating cost is currently a limitation to industrialize microbial lipids production by the yeast Lipomyces starkeyi. To explore economic fermentation technology, the two-stage fermentation of Lipomyces starkeyi using yeast extract peptone dextrose (YPD) medium, orange peel (OP) hydrolysate medium, and their mixed medium were investigated for seven days by monitoring OD600 values, pH values, cell growth status, C/N ratios, total carbon concentration, total nitrogen concentration, residual sugar concentration, lipid content, lipid titer, and fatty acids profiles of lipids. The results showed that two-stage fermentation with YPD and 50% YPD + 50% OP medium contributed to lipid accumulation, leading to larger internal lipid droplets in the yeast cells. However, the cells in pure OP hydrolysate grew abnormally, showing skinny and angular shapes. Compared to the one-stage fermentation, the two-stage fermentation enhanced lipid contents by 18.5%, 27.1%, and 21.4% in the flasks with YPD medium, OP medium, and 50%YPD + 50%OP medium, and enhanced the lipid titer by 77.8%, 13.6%, and 63.0%, respectively. The microbial lipids obtained from both one-stage and two-stage fermentation showed no significant difference in fatty acid compositions, which were mainly dominated by palmitic acid (33.36–38.43%) and oleic acid (46.6–48.12%). Hence, a mixture of commercial medium and lignocellulosic biomass hydrolysate could be a promising option to balance the operating cost and lipid production.

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Section: Resultssupporting

confidence: 92%

Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel

et al. 2021

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“…In order to mimic the same C:N ratio presented in RD 1, the C:N ratio in SM was set at 12 (1.3 g N‐NH 4 + /L). Under such low C:N ratio, the expected lipid accumulation would be low, considering the importance of high C:N ratios when using both sugar‐based and SCFAs‐rich media for oleaginous yeast fermentation (Amza et al, 2019 ; Morales‐Palomo, González‐Fernández, & Tomás‐Pejó, 2022 ; Wei et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…It has been suggested that R. toruloides could have a similar mechanism to Rhodosporillum rubrum to inhibit cell growth and redirect the carbon flux toward lipid accumulation under SO 4 2− limitation, which could explain the higher accumulation of lipids with such nutrient limitation (Melnicki et al, 2016). Moreover, Wu et al, (2011) used glucose as carbon source, which is assimilated via de novo pathway (Amza et al, 2019), whereas SCFAs used in this study are probably mainly assimilated via ex novo pathway (Papanikolaou & Aggelis, 2011) which could result on a different effect of SO 4 2− limitation. Independently of the SO 4 2− level, lipid yields (Table 2) (0.06 g/g -0.14 g/g) were quite low when compared with those previously reported from sugars or even SCFAs.…”

Section: Effect Of Somentioning

confidence: 99%

Phosphate limitation as crucial factor to enhance yeast lipid production from short‐chain fatty acids

Morales-Palomo

Tomás‐Pejó

González‐Fernández

2022

Microbial Biotechnology

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Microbial lipids for chemical synthesis are commonly obtained from sugar‐based substrates which in most cases is not economically viable. As a low‐cost carbon source, short‐chain fatty acids (SCFAs) that can be obtained from food wastes offer an interesting alternative for achieving an affordable lipid production process. In this study, SCFAs were employed to accumulate lipids using Yarrowia lipolytica ACA DC 50109. For this purpose, different amounts of SCFAs, sulfate, phosphate and carbon: phosphate ratios were used in both synthetic and real SCFAs‐rich media. Although sulfate limitation did not increase lipid accumulation, phosphate limitation was proved to be an optimal strategy for increasing lipid content and lipid yields in both synthetic and real media, reaching a lipid productivity up to 8.95 g/L h. Remarkably, the highest lipid yield (0.30 g/g) was achieved under phosphate absence condition (0 g/L). This fact demonstrated the suitability of using low‐phosphate concentrations to boost lipid production from SCFAs.

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“…An internal standard C8:0 (octanoic acid) was included in each sample and FAME was detected at the provided retention time (Supplementary Table S1). The amount of FAME (%) was calculated as the percentage of each fatty acid to the total weight of fatty acids produced [23].…”

Section: Methodsmentioning

confidence: 99%

Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification

et al. 2020

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Nanofiltration membrane separation is an energy-saving technology that was used in this study to concentrate extracellular lipase and increase its total activity for biodiesel production. Lipase was produced by recombinant Aspergillus oryzae expressing Fusarium heterosporum lipase (FHL). A sulfonated polyethersulfone nanofiltration membrane, NTR-7410, with a molecular weight cut-off of 3 kDa was used for the separation, because recombinant lipase has a molecular weight of approximately 20 kDa, which differs from commercial lipase at around 30 kDa for CalleraTM Trans L (CalT). After concentration via nanofiltration, recombinant lipase achieved a 96.8% yield of fatty acid methyl ester (FAME) from unrefined palm oil, compared to 50.2% for CalT in 24 h. Meanwhile, the initial lipase activity (32.6 U/mL) of recombinant lipase was similar to that of CalT. The composition of FAME produced from recombinant concentrated lipase, i.e., C14:1, C16:0, C18:0, C18:1 cis, and C18:2 cis were 0.79%, 34.46%, 5.41%, 45.90%, and 12.46%, respectively, after transesterification. This FAME composition, even after being subjected to nanofiltration, was not significantly different from that produced from CalT. This study reveals the applicability of a simple and scalable nanofiltration membrane technology that can enhance enzymatic biodiesel production.

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High cell density cultivation of Lipomyces starkeyi for achieving highly efficient lipid production from sugar under low C/N ratio

Cited by 19 publications

References 30 publications

Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel

Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel

Phosphate limitation as crucial factor to enhance yeast lipid production from short‐chain fatty acids

Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification

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