Concentration of Lipase from Aspergillus oryzae Expressing Fusarium heterosporum by Nanofiltration to Enhance Transesterification

Wijaya, Hans; Sasaki, Kengo; Kahar, Prihardi; Quayson, Emmanuel; Rachmadona, Nova; Amoah, Jerome; Hama, Shinji; Ogino, Chiaki; Kondo, Akihiko

doi:10.3390/pr8040450

Cited by 6 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, they concluded that both enzyme-rich solutions can be efficiently reused (achieving FAME yields of around 80%-83%), and that the ceramic membranes tested did not exhibit any deterioration concerning their performance behavior. Similarly, Wijaya et al (2020) investigated the use of a sulfonated polyethersulfone nanofiltration membrane (3 kDa MWCO) for the condensation of an extracellular lipase expressed from A. oryzae. After nanofiltration, the concentrate stream presented an 83% increased lipase activity against the initial (unconcentrated) lipase solution.…”

Section: Membrane Post-treatment Processes In Enzymatic Transesterifi...mentioning

confidence: 99%

Novel concepts for the biocatalytic synthesis of second-generation biodiesel

Spanou,

Moschona,

Theodosiou

et al. 2024

Front. Catal.

View full text Add to dashboard Cite

Biodiesel is synthesized by the transesterification of triglycerides of oils with short-chain alcohols, such as methanol and ethanol. According to the Renewable Energy Directive guidelines (RED II 2018/2001/EU) the contribution of advanced biofuels, which do not include edible oils, towards the overall EU target, is at 1% in 2025 and at least 3.5% in 2030. Bioprocesses that valorize non-edible oils for the production of second-generation biodiesel could play a critical role in achieving this goal. Immobilized lipases, as well as other enzyme classes, such as cutinases and acyltransferases, are utilized as biocatalysts for this process. For the sustainability of the process, renewable materials can be used as immobilization matrices, or even enzymes anchored on the cells as whole-cell biocatalysts. Membrane reactors can also be employed to facilitate the enzymatic transesterification by conducting a continuous enzymatic reaction and simultaneously separate the products in a single operation. The advances on the aforementioned fast-pacing fields are presented in this work.

show abstract

Section: Membrane Post-treatment Processes In Enzymatic Transesterifi...mentioning

confidence: 99%

Novel concepts for the biocatalytic synthesis of second-generation biodiesel

Spanou,

Moschona,

Theodosiou

et al. 2024

Front. Catal.

View full text Add to dashboard Cite

show abstract

“…Reinehr et al [23] adopted ultrafiltration and microfiltration to concentrate lipase produced from Aspergillus niger. Wijiya et al [24] used a sulfonated polyether sulfone nanofiltration membrane, with a molecular weight cut-off of 3 kDa, to concentrate lipase produced from recombinant A. oryzae. The total enzymatic activity increased at about fivefold after being separated by the membrane.…”

Section: Free Lipase-catalyzed Processmentioning

confidence: 99%

Progress in Enzymatic Biodiesel Production and Commercialization

Lv¹,

Dai

et al. 2021

Processes

View full text Add to dashboard Cite

Enzymatic biodiesel production has attracted tremendous interest due to its well-recognized advantages. However, high enzyme costs limit the application of enzymatic processes in industrial production. In the past decade, great improvements have been achieved in the lab and the industrial scale, and the production cost of the enzymatic process has been reduced significantly, which has led to it being economically competitive compared to the chemical process. This review introduces the progress achieved in enzymatic biodiesel preparation and commercialization, including reducing enzyme cost, expanding low-quality raw materials, and novel reactor designs. Finally, the advantages and disadvantages of different enzymatic processes are compared.

show abstract

“…Response surface methodology was used to obtain a high concentration of the GABA strain under optimal fermentation conditions. There is also a study on nanofiltration membrane separation to concentrate lipase for biodiesel production [5]. The nanofiltration membrane technology developed showed a comparable fatty acid methyl ester (FAME) composition to that of commercial lipase, which improves its applicability and scalability.…”

mentioning

confidence: 99%