Phosphate limitation as crucial factor to enhance yeast lipid production from short‐chain fatty acids

Abstract

Microbial lipids for chemical synthesis are commonly obtained from sugar‐based substrates which in most cases is not economically viable. As a low‐cost carbon source, short‐chain fatty acids (SCFAs) that can be obtained from food wastes offer an interesting alternative for achieving an affordable lipid production process. In this study, SCFAs were employed to accumulate lipids using Yarrowia lipolytica ACA DC 50109. For this purpose, different amounts of SCFAs, sulfate, phosphate and carbon: phosphate ratios were used in both synthetic and real SCFAs‐rich media. Although sulfate limitation did not increase lipid accumulation, phosphate limitation was proved to be an optimal strategy for increasing lipid content and lipid yields in both synthetic and real media, reaching a lipid productivity up to 8.95 g/L h. Remarkably, the highest lipid yield (0.30 g/g) was achieved under phosphate absence condition (0 g/L). This fact demonstrated the suitability of using low‐phosphate concentrations to boost lipid production from SCFAs.

Cutaneotrichosporon curvatum and Yarrowia lipolytica as key players for green chemistry: efficient oil producers from food waste via the carboxylate platform

“…Agreeing with previous reports in which hydrodynamic cavitation was applied as pretreatment of livestock wastewater [ 29 ], struvite formation resulted in a marked decrease in phosphorous (50% removal). This phosphorous removal also gave rise to an increase in C:P ratio that has been recently proven to be a promising strategy for improve lipid production [ 30 , 31 ]. Yeast was not affected by any other potential by-products released in the treatment and no additional clearance steps would be required for its application.…”

Biodiesel Production Processes with Yeast: A Sustainable Approach

“…The production of second, third, and fourth-generation biodiesel involves the use of oleaginous microorganisms that must accumulate between 20% and 70% of the lipid content in their dry weight [10,20,21]. These microorganisms include certain species of bacteria, algae, and fungi [22].…”

“…As shown in Figures 1 and 2, the most widely used yeasts from the Ascomycota Phylum include Yarrowia lipolytica, Lipomyces starkeyi, Saccharomyces pastorianus, Candida tropicalis, and Metschnikowia pulcherrima. From the Basidiomycota Phylum, species such as Cutaneotrichosporon sp., Rhodotorula sp., Naganishia albida, Phenoliferia glacialis, and Sporobolomyces shibatanus [15,18,19,21,24,26,27,32,35,. All the yeasts reported in Figures 1 and 2 can produce intracellular lipids through the degradation of various substrates used as carbon sources and generate these compounds in different proportions.…”

Lipid Production from Native Oleaginous Yeasts Isolated from Southern Chilean Soil Cultivated in Industrial Vinasse Residues

“…Several authors have reported lipid accumulation in yeasts [11,13,14] with the most promising species for fat production being the following: Rhodosporidium toruloides, Lipomyces starkeyi, Lipomyces tetrasporus, Cutaneotrichosporon curvatum, Candida diddensiae, Metschnikowia reukaufii, Candida tropicalis, Solicoccosyma terricola, Naganishia albida, Papiliotrema laurentii, Rhodotorula glutinis, Rhodotorula graminis, Rhodotorula mucilaginosa, Apiotrichum domesticum, Trichosporon asahii, Tausonia pullulans, Yarrowia lipolytica and Schwanniomyces etchellsii [15][16][17][18][19]. Most of these yeasts accumulate lipids mainly in the cytoplasmic membrane (about 25% of the cell dry weight) in the form of triglycerides (TGs) [20], noting that the major production of these TGs is under conditions of nutrient limitation such as nitrogen, phosphorus and an excess carbon source [21][22][23].…”