High antibacterial performance of electrospinning silk fibroin/gelatin film modified with graphene oxide‐sliver nanoparticles

Abstract

In this study, graphene oxide-sliver nanoparticle (GO-AgNP) composite was synthesized in situ with GO as the raw material. Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy, and scanning electron microscopy were used to characterize the composite, the spherical Ag particles with a diameter of about 36 nm were well deposited on the surface of GO nanosheets without serious agglomeration, and the antibacterial properties of the composites were also tested. Moreover, the silk fibroin (SF)/gelatin (GT) electrospun nanofiber film was prepared by electrospinning, and the structure of the SF nanofiber film was observed using a Fourier transform infrared spectrometer, X-ray diffractometer, and scanning electron microscope. The TGA curves indicated that the total weight loss rate of SF nanofibers at 400 C was significantly higher than that of SF/GT composite nanofibers (74.72% for pure SF, 62.37% for SF/GT nanocomposites). Finally, the GO-AgNP composite was combined with electrospinning SF film, which resulted in the decrease in surface roughness from 393.5 AE 123.7 nm to 109.9 AE 24.43 nm and the decrease in contact angle from 82.48 to 54.78 . Besides, the GO-AgNP composites enhanced the antibacterial performance of SF film greatly, which was conducive to its application in biological tissue engineering.

Photocrosslinking silver nanoparticles–aloe vera–silk fibroin composite hydrogel for treatment of full-thickness cutaneous wounds

“…The TGA results showed that the Ag–AV–SF hydrogel had excellent thermostability indicating that the integrity of the structure was maintained under the conditions of preservation and practical application. The thermal properties of the Ag–AV–SF hydrogel showed great improvement for synergistic effect [ 34 ]. The Ag+ release rate in neutral (pH = 7.4) and acidic environment (pH = 6.5) for 5 days is shown in Fig.…”

Exploration of the Wound Healing Potential of Thermoplastic Polyurethane Electrospun Membrane Incorporated with Phenolic Acids in Spenceria ramalana Trimen

“…The antibacterial ability of fibrous membrane was attributed to sliver nanoparticles, which had been proven by many studies . [ 28,29 ] The inhibition zone of TPU/AgNPs/TPA membrane against three strains of bacteria was slightly larger than that of TPU/AgNPs (Table S1, Supporting Information). And the antibacterial activity of TPU/AgNPs/TPA fibrous membrane against drug‐resistant E. coli was significantly higher than that against standard strain of E. coli (Table S1, Supporting Information).…”

“…In NC and TPU group, the inflammatory cells decreased and the epidermis at wound site were incomplete. According to the previous articles, [ 29 ] inflammation could appear negative influence on granulation tissue formation and wound closure. The sustained release of phenolic acid and the present of AgNPs at wound site contributed to anti‐inflammatory, antibacterial activity, antioxidant and accelerating epithelial hyperplasia.…”

Biomedical Applications of Electrospun Graphene Oxide

“…GO-containing electrospun mats have been vastly reported as antimicrobial and antibacterial (Table ). These mats have been constructed with various polymers such as polyurethane/cellulose acetate, polycaprolactone, fibroin, silk fibroin, , gelatin, , chitosan, ,, PVA, ,− PVP, PEO, collagen, etc. Generally, Gram-positive and Gram-negative bacteria are killed when GO is added into the scaffold ,,,,,,, (Figure ), with one report highlighting its efficiency toward Gram-negative bacteria in particular …”