Hierarchical Au nanoarrays functionalized 2D Ti2CTx MXene membranes for the detection of exosomes isolated from human lung carcinoma cells

You, Qiannan; Zhuang, Linlin; Chang, Zhimin; Ge, Ming‐Feng; Mei, Qian; Li, Yang; Dong, Wen‐Fei

doi:10.1016/j.bios.2022.114647

Cited by 34 publications

(17 citation statements)

References 37 publications

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“…The linear regression equation was ΔI = 0.0418logC Exos −0.0238 (R 2 = 0.989), and the LOD was 42 particles/μL (S/N = 3). This indicates that the sensor has a high sensitivity [ 52 , 53 ].…”

Section: Resultsmentioning

confidence: 99%

Room-Temperature Self-Healing Conductive Elastomers for Modular Assembly as a Microfluidic Electrochemical Biosensing Platform for the Detection of Colorectal Cancer Exosomes

Wang

Zhang

et al. 2023

Micromachines

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Modular components for rapid assembly of microfluidics must put extra effort into solving leakage and alignment problems between individual modules. Here, we demonstrate a conductive elastomer with self-healing properties and propose a modular microfluidic component configuration system that utilizes self-healing without needing external interfaces as an alternative to the traditional chip form. Specifically, dual dynamic covalent bond crosslinks (imine and borate ester bonds) established between Polyurethane (PU) and 2-Formylbenzeneboronic acid (2-FPBA) are the key to a hard room-temperature self-healing elastomeric substrate PP (PU/2-FPBA). An MG (MXene/GO) conductive network with stable layer spacing (Al-O bonds) obtained from MXene and graphene oxide (GO) by in situ reduction of metals confers photothermal conductivity to PP. One-step liquid molding obtained a standardized modular component library of puzzle shapes from PP and MGPP (MG/PP). The exosomes were used to validate the performance of the constructed microfluidic electrochemical biosensing platform. The device has a wide detection range (50–105 particles/μL) and a low limit of detection (LOD) (42 particles/μL) (S/N = 3), providing a disposable, reusable, cost-effective, and rapid analysis platform for quantitative detection of colorectal cancer exosomes. In addition, to our knowledge, this is the first exploration of self-healing conductive elastomers for a modular microfluidic electrochemical biosensing platform.

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Section: Resultsmentioning

confidence: 99%

Room-Temperature Self-Healing Conductive Elastomers for Modular Assembly as a Microfluidic Electrochemical Biosensing Platform for the Detection of Colorectal Cancer Exosomes

Wang

Zhang

et al. 2023

Micromachines

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“…Figure 2 C shows the morphology of the prepared MXene-Ti 3 C 2 T x with thin and transparent nanoflakes stacked on each other. The layer spacing is wider than before the etching [ 24 , 25 ]. The structural characteristics of the as-prepared MXene-Ti 3 C 2 T x and Ti 3 C 2 MXenes composite structures were investigated by performing XRD analysis, as shown in Figure 2 B.…”

Section: Resultsmentioning

confidence: 99%

“…Wu et al developed a biosensor based on the amino functionalization of Ti 3 C 2 MXene for the detection of tumor biomarkers [ 23 ]. In addition, due to the abundant oxygen or hydroxyl terminated surface, Ti 3 C 2 can interact with a majority of biomolecules in many ways, which make them unique nanobiointerface units for biosensor construction [ 24 ]. Therefore, AuPt DNs combined with Ti 3 C 2 MXene were used as the matrix nanoprobe to selectively and reproducibly detect exosomes, and AuPt DNs showed excellent catalytic and conductive properties.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Micro-Immunosensor of Cubic AuPt Dendritic Nanocrystals/Ti3C2-MXenes for Exosomes Detection

Wei

Wang

et al. 2023

Micromachines

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Exosomes are extracellular vesicles that exist in body circulation as intercellular message transmitters. Although the potential of tumor-derived exosomes for non-invasive cancer diagnosis is promising, the rapid detection and effective capture of exosomes remains challenging. Herein, a portable electrochemical aptasensor of cubic AuPt dendritic nanocrystals (AuPt DNs)/Ti3C2 assisted in signal amplification, and aptamer CD63 modified graphene oxide (GO) was immobilized on a screen-printed carbon electrode (SPCE) as the substrate materials for the direct capture and detection of colorectal carcinoma exosomes. Cubic AuPt DNs/Ti3C2 was synthesized according to a simple hydrothermal procedure, and the AuPt DNs/Ti3C2-Apt hybrid demonstrated an efficient recognition of exosomes. Under optimal conditions, a detection limit of down to 20 exosomes µL−1 was achieved with the linear range from 100 exosomes μL−1 to 5.0 × 105 exosomes μL−1. The proposed immunosensor could be suitable for the analysis of exosomes and has clinical value in the early diagnosis of cancer.

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“…For instance, exosome MicroRNA-103 can promote proliferation and invasion of liver cancer cells ( 145 ). Numerous studies have shown that abnormalities in the secretion of exosomes occur during the development of cancer, providing a theoretical basis for the use of exosomes in early cancer screening ( 146 ). Recent studies have found that exosomes derived from bone marrow MSCs carry ncRNAs that can suppress cancer, including lncRNA PTENP1, which can suppress bladder cancer, and microRNA-551b-3p, which can suppress breast cancer.…”

Section: Discussionmentioning

confidence: 99%

Natural products exert anti-tumor effects by regulating exosomal ncRNA

Liu

Guan

et al. 2022

Front. Oncol.

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Currently, more than 60% of the approved anti-cancer drugs come from or are related to natural products. Natural products and exosomal non-coding RNAs (ncRNAs) exert anti-cancer effects through various regulatory mechanisms, which are of great research significance. Exosomes are a form of intercellular communication and contain ncRNAs that can act as intercellular signaling molecules involved in the metabolism of tumor cells. This review exemplifies some examples of natural products whose active ingredients can play a role in cancer prevention and treatment by regulating exosomal ncRNAs, with the aim of illustrating the mechanism of action of exosomal ncRNAs in cancer prevention and treatment. Meanwhile, the application of exosomes as natural drug delivery systems and predictive disease biomarkers in cancer prevention and treatment is introduced, providing research ideas for the development of novel anti-tumor drugs.

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Hierarchical Au nanoarrays functionalized 2D Ti2CTx MXene membranes for the detection of exosomes isolated from human lung carcinoma cells

Cited by 34 publications

References 37 publications

Room-Temperature Self-Healing Conductive Elastomers for Modular Assembly as a Microfluidic Electrochemical Biosensing Platform for the Detection of Colorectal Cancer Exosomes

Room-Temperature Self-Healing Conductive Elastomers for Modular Assembly as a Microfluidic Electrochemical Biosensing Platform for the Detection of Colorectal Cancer Exosomes

Electrochemical Micro-Immunosensor of Cubic AuPt Dendritic Nanocrystals/Ti3C2-MXenes for Exosomes Detection

Natural products exert anti-tumor effects by regulating exosomal ncRNA

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