Hexavalent chromate reductase activity in cytosolic fractions of Pseudomonas sp. G1DM21 isolated from Cr(VI) contaminated industrial landfill

Desai, Chirayu; Jain, Kunal; Madamwar, Datta

doi:10.1016/j.procbio.2008.02.015

Cited by 101 publications

(77 citation statements)

References 49 publications

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“…Additionally, our strain can be considered as a mesophile bacterium with a moderate optimum temperature. Such growth dependent chromate reduction has also been earlier reported by Desai et al (2008a). It has been reported that the optimal temperature of Cr (VI) reduction could be in the range of 25-37 ºC (Ibrahim et al, 2012).…”

Section: Discussionsupporting

confidence: 83%

Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant

Nguema¹,

Luo

Lian³

2014

IJB

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Hexavalent chromium is a toxic heavy metal in environment. To remove it from the system, reduction to the trivalent form using microorganisms is an alternative cost-effective method compared to the conventional physicochemical methods. Bacillus cereus Pf-1was isolated and tested for the reduction potential of Cr (VI) using different sub-cellular fractions. Diphenylcarbazide method was used to measure the remaining Cr (VI) concentration in supernatant after centrifugation, and the pellet was used for Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray (EDX) analysis. Almost complete reduction of Cr (VI) was reported for initial Cr (VI) concentration from 10-50 mg/L. Assay with permeabilized cells (treated with Toluene and Triton X-100) and cells free assay demonstrated that Cr (VI) reduction activity was mainly associated with the cytoplasmic fraction of the cells. Considering the major amount of Cr (VI) being reduced within 24 h, the chromate reductase could have been released during growth. Additionally, SEM analysis showed that Bacillus cereus treated with Cr (VI) grew well and had uniform size. EDX analysis revealed certain amounts of chromium in cells treated with Cr (VI). Based on this, Bacillus cereus Pf-1 could be considered as a potential reducing agent for Cr (VI) in aqueous solutions.

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Section: Discussionsupporting

confidence: 83%

Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant

Nguema¹,

Luo

Lian³

2014

IJB

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“…Chromium reductase enzyme isolated from Escherichia coli ATCC 33456 showed maximum activity at pH 6.5 [13] whereas in Bacillus sp. ES29 (CRB) [19] and Pseudomonas G1DM21 [23] maximum enzymatic activity was found to be at neutral pH (pH 7).…”

Section: Resultsmentioning

confidence: 99%

Preliminary Characterization of a NADPH Dependent Chromate Reductase from Trichoderma pseudokoningii

2016

J Microbiol Microb Technol

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Intracellular chromate reductase enzyme extracted from a chromium tolerant strain Trichoderma pseudokoningii was partially purified by ultrafiltration and gel filtration chromatography. The purified fraction showed highest reducing activity in presence of 1% (w/v) hexavalent Chromium. NADPH at a conentration of 2 mM was found to act as electron donor in the reduction reaction. The intracellular chromate reductase was found to have an optimum pH and temperature of 7.4 and 70 °C respectively. The enhancement of reductase activity in presence of exogenous thiols indicated the presence of thiols at active site.

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“…The inhibiting effect on the decreasing the performance of bacteria might be due to the reason of toxic effect of these contaminants at increased concentration for bacterial cells (Dhal et al, 2010;Dey and Paul, 2012). Another important factor for this reduction can be the due to the production of morphological changes and oxidative stress on bacterial cell (Desai et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Hexavalent Cr and azo dyes exhibits same properties such as water soluble, persistence, carcinogenic and mutagenic (Moosvi et al, 2007;Desai et al, 2008). Hence their combine existence in tannery and textile effluents would have synergistic effect in poisoning the environment.…”

Section: Introductionmentioning

confidence: 99%

“…Many microorganisms has already been reported with developed enzymatic system for individual or simultaneous degradation of azo dyes and Cr system (Pandey et al, 2007;Desai et al, 2008;Khalid et al, 2012;Mahmood et al, 2013).Therefore, these bacterial strains can be helpful under such conditions to decolorize azo dyes. Hence, the present study was designed by using our two previously reported bacterial species Psychrobacter and Klebsiella to determine the decolorization behavior of these species in the presence of different forms of Cr.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Chromium Forms on the Biodegradation of Reactive Black-5 Azo Dye by Psychrobacter and Klebsiella species

Khalid¹,

Arshad²,

Mahmood³

et al. 2015

IJAB

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To cite his paper: Khalid, A., J. Arshad, S. Mahmood, I. Aziz and M. Arshad, 2015 AbstractAzo dyes and chromium (Cr) are released in the wastewater of leather processing industry during tanning process. Several species of bacteria have shown ability to degrade azo dyes individually; however, presence of Cr ions in industrial effluents may affect the bacterial efficiency of removing azo dyes from waste water. In this study, biodegradation of reactive black-5 dye was studied in a mineral salts medium (MSM) liquid culture in the presence of Cr ions. Two bacterial strains Psychrobacter alimentarius KS23 and Klebsiella oxytoca N7 capable of degrading azo dyes were used individually as well as in a consortium for the degradation of azo dyes. Cr was applied in the form of K2Cr2O7, CrCl3, Cr2(SO4)3 at different concentrations (ranging from 0-250 mg L -1 ) of Cr. The results showed that presence of Cr in mineral salt medium significantly inhibited the azo dye decolorization. In the absence of Cr salts, complete decolorization of 100 mg dye L -1 was achieved by the bacterial strains in 8-12 h while up to 83% decolorization occurred in 72 h at 100 mg L -1 Cr. The rate of decolorization considerably decreased with increasing concentration of azo dye in the presence of 50 mg Cr L -1 medium. These findings imply that the existence of Cr in wastewater can significantly affect the bacterial efficiency in wastewater treatment systems to degrade azo dyes.

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Hexavalent chromate reductase activity in cytosolic fractions of Pseudomonas sp. G1DM21 isolated from Cr(VI) contaminated industrial landfill

Cited by 101 publications

References 49 publications

Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant

Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant

Preliminary Characterization of a NADPH Dependent Chromate Reductase from Trichoderma pseudokoningii

Effect of Chromium Forms on the Biodegradation of Reactive Black-5 Azo Dye by Psychrobacter and Klebsiella species

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