Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant

Nguema, Paul Fabrice; Luo, Zhaohua; Lian, Jing

doi:10.5539/ijb.v6n2p64

Cited by 7 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reduction to Cr (III) results in the formation of insoluble precipitate [Cr(OH)3], which is easily removed from wastewater (Jeyasingh and Philip, 2005). The enzyme chromium reductase found in P. ambigua (Campos-Garcia et al, 1997), Bacillus sp., (Wang et al, 1991) and Bacillus cereus Pf-1 (Nguema et al, 2014) were purified and characterized. More recently, to clone a chromate reductase gene, novel soluble chromate reductase of P. putida was purified to homogeneity and characterized (Puzon et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

CR (VI) Removal by Indigenous Klebsiella Species PB6 Isolated from Contaminated Soil under the Influence of Various Factors

Wani¹,

Omozele²

2015

Current Research in Bacteriology

View full text Add to dashboard Cite

Chromium (VI) contamination has accelerated due to rapid industrialization worldwide. Aim of this study is to check the bacterial species for their tolerance towards chromium (VI), chromium (VI) reduction under various pH and further check whether these bacteria are reducing Cr (VI) under the influence of various metals, carbon source and protein denaturant. Bacterial strains were isolated from metal contaminated soils of Abeokuta. All of the isolates showed tolerance to chromium (VI). Among all the strains, only Klebsiella spp. PB6 showed reduction of chromium (VI). Maximum reduction (89.5%) of chromium (VI) was observed at pH 7 by Klebsiella spp. PB6. Similarly, Klebsiella spp. PB6 also reduced the chromium considerably at pH 6 (88%) and pH 8 (71%) at a concentration of 100 μg Cr mLG 1 after 120 h of incubation. Klebsiella spp. PB6 also showed chromium (VI) reduction under various metals, protein denaturant and carbon source. There was maximum decrease in Cr (VI) reduction under the influence of ZnCl 2 which was followed by PbCl 2. Urea and citrate also decreased Cr (VI) reduction compared to control cells. Due to above properties strains could therefore be used as bioremediators of metals in soils contaminated with heavy metals.

show abstract

Section: Introductionmentioning

confidence: 99%

CR (VI) Removal by Indigenous Klebsiella Species PB6 Isolated from Contaminated Soil under the Influence of Various Factors

Wani¹,

Omozele²

2015

Current Research in Bacteriology

View full text Add to dashboard Cite

show abstract

“…The reduction/ detoxification of Cr (VI) by microbes is, however, inexpensive and environmentally safe approach and provides a viable option to protect the environment from chromium toxicity. The reduction of Cr (VI) is reported in Bacillus (Nguema et al, 2014;Elangovan et al, 2006;Chaturvedi, 2011), Pseudomonas sp. (Wani and Ayoola, 2015;Rahman et al, 2007), Escherichia coli (Bae et al, 2005), Microbacterium (Pattanapipitpaisal et al, 2001b), Ochrobactrum intermedium (Faisal and Hasnain, 2005) and Micrococcus (Sultan and Hasnain, 2005).…”

Section: Introductionmentioning

confidence: 99%

Cr (VI) Reduction by Indigenous Bacillus Species PB5 Isolated from Contaminated Soil of Abeokuta Ogun State, Nigeria

Wani¹,

Omozele²,

Wasiu³

et al. 2015

International J. of Soil Science

View full text Add to dashboard Cite

Chromium (VI) contamination has accelerated due to rapid industrialization worldwide. Aim of this study is to check the bacterial species for their tolerance towards chromium (VI), chromium (VI) reduction under various pH and further check whether these bacteria are reducing Cr (VI) und er the influence of various metals, carbon source and protein denaturant. Bacterial strains were isolated from metal contaminated soils of Abeokuta. All of the isolates showed tolerance to chromium (VI). Among all the strains, only Bacillus species PB5 showed reduction of Chromium (VI). Maximum reduction (90%) of chromium (VI) was observed at pH 7 by Bacillus species PB5. Similarly, Bacillus species PB5 also reduced the chromium considerably at pH 6 (86%) and pH 8 (87.5%) at a concentration of 100 μg Cr mLG 1 after 120 h of incubation. Bacillus species PB5 also showed chromium (VI) reduction under various metals, protein denaturant and carbon source. There was maximum decrease in Cr (VI) reduction under the influence of PbCl 2 which was followed by ZnCl 2. Urea and citrate also decreased Cr (VI) reduction compared to control cells. Due to above properties strains could therefore be used as bioremediators of metals in soils contaminated with heavy metals.

show abstract

“…The bacterial strain, Pf-1 tolerant to Cr (VI) was isolated in dewatering sludge collected from a sewage treatment plant, Wuhan, China by enrichment culture technique (Nguema et al, 2014). The culture was grown in Nutrient Broth (NB) agar plates previously sterilized amended with 1 mM Cr (VI) as K 2 Cr 2 O 7 .…”

Section: Bacterial Strain Identification and Growth Conditionsmentioning

confidence: 99%

Treatment of Chromium Pollution by the Reductase Enzyme Generated by a Chromium-Resistant Bacterium Isolated in Dewatering Sludge

Nguema

Luo²,

Mounir³

et al. 2017

IJB

Self Cite

View full text Add to dashboard Cite

An efficient Cr (VI)-resistant and reducing bacterial strain was successfully isolated in dewatering sludge collected from a sewage treatment plant, and characterized in vitro Cr (VI) reduction through a reductase enzyme. Phylogenetic analysis using 16S rDNA gene sequencing revealed that the newly isolated strain namely Pf-1 was closely related to Bacillus cereus. The strain almost reduced 0.17 mM Cr (VI) within 24h incubation and the presence of different substrates such as glucose, sucrose, or acetate significantly enhanced the reduction rate of Cr (VI) to Cr (III). However, addition of the same substrate at the stationary phase of the microbial growth increased the reduction rate as well as bacterial growth. Additionally, raising the concentration of thiosulfate in the medium doubled the reduction rate under similar conditions. Assay with different fractions of the cells demonstrated that the reductase enzyme activity was mainly associated with the cytoplasmic fraction. The maximum activity was 23.3 µM h -1 mg -1 protein and was obtained at the Cr (VI) concentration of 2 mM. The promising strain can be efficiently employed for bioremediation of Cr (VI) polluted sites.

show abstract

Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant

Cited by 7 publications

References 37 publications

CR (VI) Removal by Indigenous Klebsiella Species PB6 Isolated from Contaminated Soil under the Influence of Various Factors

CR (VI) Removal by Indigenous Klebsiella Species PB6 Isolated from Contaminated Soil under the Influence of Various Factors

Cr (VI) Reduction by Indigenous Bacillus Species PB5 Isolated from Contaminated Soil of Abeokuta Ogun State, Nigeria

Treatment of Chromium Pollution by the Reductase Enzyme Generated by a Chromium-Resistant Bacterium Isolated in Dewatering Sludge

Contact Info

Product

Resources

About