Heterologous recombinant protein with decapacitating activity prevents and reverts cryodamage in ram sperm: An emerging biotechnological tool for cryobiology

Zalazar, Lucía; Ledesma, Alba; Hozbor, F.; Cesari, Andreína

doi:10.1016/j.anireprosci.2015.11.007

Cited by 16 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, attempts are being made to determine markers of suitability for freezing ejaculates. Seminal plasma proteins and some other compounds have been used as markers of fertility in bulls (Moura et al, 2006) and semen freezability in rams (Zalazar et al, 2016) and…”

Section: Discussionmentioning

confidence: 99%

A new sperm selection criterion for cryopreservation of boar semen

Trzcińska

Bryła

2021

Annals of Animal Science

View full text Add to dashboard Cite

This study aimed to define potential markers that could determine the suitability of ejaculate for cryopreservation. Fresh semen from eleven boars (4–7 ejaculates/boar), regardless of their sperm motility, was subjected to a cryopreservation procedure. The sperm quality before and after freezing was assessed based on the sperm membrane permeability and acrosome integrity. The results showed that it was possible to effectively cryopreserve ejaculates below the accepted standards of 70–80% of fresh motile sperm and still obtain a high cryosurvival rate. Moreover, a significant correlation was found between the percentage of viable sperm with apoptotic-like changes, viable sperm with reacted acrosomes, and the cryosurvival rate. The proposed markers for assessing the quality of fresh semen could be used to predict the success of cryopreservation procedures.

show abstract

Section: Discussionmentioning

confidence: 99%

A new sperm selection criterion for cryopreservation of boar semen

Trzcińska

Bryła

2021

Annals of Animal Science

View full text Add to dashboard Cite

show abstract

“…Supplementation with SP can be applied before freezing or after thawing. Our group has been successful in improving ram sperm parameters by adding SP or their active components after thawing and selection, probably because of prompting sperm ‘stabilizing molecules’ direct interactions (Bernardini et al, ; Dominguez et al, ; Ledesma et al, ; Zalazar, Ledesma, Hozbor, & Cesari, ). Moreover, we and other researchers have recently demonstrated that sperm surface is modified by extender components (Pini et al, ; Ramírez‐Vasquez, Cano, Hozbor, & Cesari, ).…”

Section: Discussionmentioning

confidence: 99%

“…As evidenced by treatments with SP added after Percoll ® washing, the addition of SP significantly increased ( p < .05) the rates of total and progressive sperm motilities with intact PM, intact acrosomes, resistance to chromatin decondensation and the proportion of non‐capacitated sperm, compared with non‐washed samples, independently of the extender used. On this regard, sperm were incubated for 15 min with SP, enough to detect the interaction between SP proteins and EY lipoproteins (Bergeron, Villemure, Lazure, & Manjunath, ; Manjunath, Nauc, Bergeron, & Menard, ) and between isolated SP proteins and the sperm surface (Ledesma et al, ; Zalazar et al, ).…”

Section: Discussionmentioning

confidence: 99%

Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm

Ramírez-Vasquez

Cesari

Greco

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

Seminal plasma (SP) proteins interact with sperm plasma membrane (PM) modulating its functionality. It has been shown that SP proteins can reverse the damage caused by freeze‐thaw; however in these studies, SP has been added to washed sperm (i.e., cells depleted from homologous SP and extender). The aim of the current study was to assess whether the egg yolk‐based extender (EY) modifies SP ability to ameliorate sperm parameters in frozen‐thawed ram spermatozoa. Ejaculates were diluted in EY or soybean lecithin‐based extender (SL) and evaluated before and after freezing to measure the cell damage according to the extender. Even when all classical parameters decreased after freezing, as expected (p < .05), there was no effect of the extender. SP treatment was applied after freeze‐thaw. Sperm were incubated with SP (20% v/v) in the presence of either EY or SL, and sperm parameters were assessed after thawing compared with the same treatments after Percoll sperm selection (washed). Treatments with 20% SP improved sperm total and progressive motility compared with controls regardless of washing and extender (p < .05); however, washed sperm showed higher percentage of total sperm motility compared with those unwashed (p < .05). Moreover, treatment with 20% SP showed significantly higher percentages of PM integrity, sperm with intact acrosomes, integrity of chromatin and non‐capacitated sperm in samples diluted with EY when washed before treatment compared with the other conditions (p < .05). It was concluded that the presence of the extenders and particularly egg yolk alters the SP capacity to reduce the cryodamage.

show abstract

“…In conclusion, elephant sperm were affected by a dilution effect and restoration of SP proportion with stallion SP improved the motility of chilled highly diluted sperm. Further identification of this supportive component may benefit the development of an appropriate extender or may allow treatment with direct addition to sperm used for AI in elephant, as has been carried out in bovine (Alvarez‐Gallardo et al., ) and in more sophisticated techniques in ram (Zalazar, Ledesma, Hozbor, & Cesari, ).…”

Section: Discussionmentioning

confidence: 99%

Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm

Pinyopummin

Mahasawangkul²,

Nunklang

et al. 2018

Reprod Domestic Animals

View full text Add to dashboard Cite

The dilution effect and effect of restoring seminal plasma (SP) proportion in diluted semen were determined in chilled Asian elephant sperm. Semen was collected from eight males, and samples with ≥30% motile sperm were used in the study. Tris-glucose-egg yolk extender (TE) was used for cooled storage at 4°C for 48 hr. In experiment 1 (n = 18), semen was diluted to 1:1, 1:3, 1:7 and 1:15 with TE (volume per volume). There were no significant changes in sperm viability and sperm with normal acrosome integrity among dilutions, but sperm motility and motility velocities were greater (p < .05) in the 1:1 dilution than those of the 1:7 and 1:15 dilutions at 48 hr of storage. In experiment 2, supplemented SP was derived from elephants and stallions. In experiment 2.1, diluted semen (1:7 dilution) was restored with SP to obtain a 1:2 proportion (n = 8). Sperm motility, viability and sperm with normal acrosome integrity were similar among treatments, but motility velocities were greater (p < .05) with stallion SP at 48 hr of storage. In experiment 2.2, diluted semen (1:15 dilution) was restored with SP to obtain a 1:3 proportion (n = 10). Sperm viability and sperm with normal acrosome integrity were similar among treatments at 48 hr of storage. However, sperm motility and motility velocities were greater (p < .05) with stallion SP than those of others. In conclusion, elephant sperm motility was affected by a dilution effect and restoration of SP proportion with stallion SP, but not with elephant SP, could improve motility in chilled highly diluted sperm.

show abstract

Heterologous recombinant protein with decapacitating activity prevents and reverts cryodamage in ram sperm: An emerging biotechnological tool for cryobiology

Cited by 16 publications

References 42 publications

A new sperm selection criterion for cryopreservation of boar semen

A new sperm selection criterion for cryopreservation of boar semen

Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm

Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm

Contact Info

Product

Resources

About