Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview

Ferreira, Rui Seabra; Barros, Luciana Curtolo de; Abbade, Luciana Patrícia Fernandes; Barraviera, Sílvia Regina Catharino Sartori; Silvares, Maria Regina Cavariani; Pontes, Letícia Gomes de; Santos, Lucilene Delazari dos; Barraviera, Benedito

doi:10.1186/s40409-017-0109-8

Cited by 65 publications

(79 citation statements)

References 54 publications

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“…To overcome these challenges, the Center for the Study of Venoms and Venomous Animals (CEVAP) from São Paulo State University (UNESP), Sao Paulo, Brazil, has been studying and developing heterologous fibrin biopolymer (HFB) from animal origin materials since the 1990s. This heterologous biomaterial is biocompatible, having hemostatic, adhesive, sealant, scaffold and drug delivery properties [9][10][11]. This bioproduct is being successfully tested in reconstruction processes when applied to various tissues [6,[12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

et al. 2020

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Bone defects cause aesthetic and functional changes that affect the social, economic and especially the emotional life of human beings. This complication stimulates the scientific community to investigate strategies aimed at improving bone reconstruction processes using complementary therapies. Photobiomodulation therapy (PBMT) and the use of new biomaterials, including heterologous fibrin biopolymer (HFB), are included in this challenge. The objective of the present study was to evaluate the influence of photobiomodulation therapy on bone tibial reconstruction of rats with biomaterial consisting of lyophilized bovine bone matrix (BM) associated or not with heterologous fibrin biopolymer. Thirty male rats were randomly separated into three groups of 10 animals. In all animals, after the anesthetic procedure, a noncritical tibial defect of 2 mm was performed. The groups received the following treatments: Group 1: BM + PBMT, Group 2: BM + HFB and Group 3: BM + HFB + PBMT. The animals from Groups 1 and 3 were submitted to PBMT in the immediate postoperative period and every 48 h until the day of euthanasia that occurred at 14 and 42 days. Analyses by computed microtomography (µCT) and histomorphometry showed statistical difference in the percentage of bone formation between Groups 3 (BM + HB + PBMT) and 2 (BM + HFB) (26.4% ± 1.03% and 20.0% ± 1.87%, respectively) at 14 days and at 42 days (38.2% ± 1.59% and 31.6% ± 1.33%, respectively), and at 42 days there was presence of bone with mature characteristics and organized connective tissue. The µCT demonstrated BM particles filling the defect and the deposition of new bone in the superficial region, especially in the ruptured cortical. It was concluded that the association of PBMT with HFB and BM has the potential to assist in the process of reconstructing bone defects in the tibia of rats.

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Section: Introductionmentioning

confidence: 99%

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

et al. 2020

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“…Commercially available fibrin biopolymers, also called fibrin sealants, consist of human fibrinogen and thrombin. The BPF used in this study is composed of a mixture of a serine protease with thrombin-like enzyme activity, purified from Crotalus durissus terrificus snake venom and buffaloes cryoprecipitate as a source of fibrinogen 53, 30 . Previous studies has shown no cytotoxicity becoming an excellent scaffold for MSC 16, 34, 54, 55 .…”

Section: Discussionmentioning

confidence: 99%

“…A new fibrin biopolymer (FBP) constituted of two animal derived compounds instead of human blood has been used in experimental biomedical applications 24,26, 27, 28, 29, 30, 31 , such as nervous tissue 32,33 and bone 34 repair as also on the treatment of chronic venous ulcers in human patients 30,33 .…”

Section: Introductionmentioning

confidence: 99%

Improvement of bone regeneration using fibrin biopolymer combined with differentiated stem cells

Creste

Orsi

Landim-Alvarenga

et al. 2019

Preprint

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Bone tissue repair remains a challenge on tissue engineering. New approaches are highly expected to regenerate fractures, bone infections, cancers and congenital skeletal abnormalities. Lately, osteoconductive biomaterials have been used with osteoprogenitor cellsas bone substitutes to accelerate bone formation. Fibrin scaffold serves as a provisional platform promoting cell migration and proliferation, angiogenesis, connective tissue formation and growth factors stimulation. When combined with mesenchymal stem cells (MSCs) maintain cell viability that exerts an immunomodulatory effect by modifying inflammatory environment through expression of pro and anti-inflammatory cytokines. We evaluated a unique heterologous fibrin biopolymer as scaffold to MSCs on bone regeneration of rat femurs. A critical-size bone defect was made in the femur and treated with fibrin biopolymer(FBP); FBP + MSC; and FBP + MSC differentiated in bone lineage (MSC-D). Bone repair was analyzed 03, 21 and 42 days later by radiographic, histological and scanning electron microscopy (SEM) imaging. The FBP+MSC-D association was the most effective treatment, since newly formed bone was more abundant and early matured in just 21 days. Our results demonstrate that FBP isolated was able to promote bone repair although cells play a crucial role on the type and quantity of bone tissue formed. We have not observed surgical site infection, inflammatory response, fractures or loss of function related with FBP. Thus, this approach can be safely expanded for clinical trials as an effort to overcome current method limitations and improve overall bone regeneration process.

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“…At the time in experimental surgery, the components were first thawed, then reconstituted, mixed, and applied according to the following protocol: The first bottle (Eppendorf®) contained fibrinogen obtained from buffalo blood (5 μl); the second contained calcium chloride (2 μl); and the last bottle contained a thrombin-like fraction (1 μl); for a total of 8 μl. The production process was described in Ferreira Junior et al [41].…”

Section: New Heterologous Fibrin Sealantmentioning

confidence: 99%

Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant

Rosso

Júnior

Buchaim

et al. 2017

Journal of Photochemistry and Photobiology B: Biology

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This research evaluated the influence of Photobiomodulation Therapy (PBMT) on lesions of the facial nerve repaired with the end-to-side technique or coaptation with a new heterologous fibrin sealant. Thirty-two Wistar rats were separated into 5 groups: Control group (CG), where the buccal branch of the facial nerve was collected; Experimental Suture Group (ESG) and Experimental Fibrin Group (EFG), in which the buccal branch was end-to-side sutured to the zygomatic branch on the right side of the face or coaptated with fibrin sealant on the left side; Experimental Suture Laser Group (ESLG) and Experimental Fibrin Laser Group (EFLG), in which the same procedures were performed as the ESG and EFG, associated with PBMT (wavelength of 830nm, energy density 6.2J/cm, power output 30mW, beam area of 0.116cm, power density 0.26W/cm, total energy per session 2.16J, cumulative dose of 34.56J). The laser was applied for 24s/site at 3 points on the skin's surface, for a total application time of 72s, performed immediately after surgery and 3 times a week for 5weeks. A statistically significant difference was observed in the fiber nerve area between the EFG and EFLG (57.49±3.13 and 62.52±3.56μm, respectively). For the area of the axon, fiber diameter, axon diameter, myelin sheath area and myelin sheath thickness no statistically significant differences were found (p<0.05). The functional recovery of whisker movement occurred faster in the ESLG and EFLG, which were associated with PBMT, with results closer to the CG. Therefore, PBMT accelerated morphological and functional nerve repair in both techniques.

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Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview

Cited by 65 publications

References 54 publications

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

Improvement of bone regeneration using fibrin biopolymer combined with differentiated stem cells

Stimulation of morphofunctional repair of the facial nerve with photobiomodulation, using the end-to-side technique or a new heterologous fibrin sealant

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