Heterologous expression of a mutant Orange gene from Brassica oleracea increases carotenoids and induces phenotypic changes in the microalga Chlamydomonas reinhardtii

Kumari, Sujata; Vira, Chaitali; Lali, Arvind; Prakash, Gunjan

doi:10.1016/j.algal.2020.101871

Cited by 22 publications

(7 citation statements)

References 33 publications

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“…The standard graph was prepared using sodium nitrate (2–20 mg l −1 ) solution. Chlorophyll a, chlorophyll b, and total carotenoids were determined as described by Kumari et al 2020 and Rathod et al 2020 at stationary phase [ 25 , 26 ]. In short, the algal cultures were centrifuged at 5000 rpm to get the pellet.…”

Section: Methodsmentioning

confidence: 99%

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Heterologous mannitol-1-phosphate dehydrogenase gene over-expression in Parachlorella kessleri for enhanced microalgal biomass productivity

Rathod

Vira

Lali

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background Microalgae have tremendous potential in CO2 sequestration, bioenergy, biofuels, wastewater treatment, and high-value metabolites production. However, large-scale production of microalgae is hampered due to photo-inhibition in outdoor cultivation. Mannitol, as an osmolyte, is known to relieve the stress produced under different abiotic stress conditions during the growth of a photosynthetic organism. Results In the present study, Mannitol-1-phosphate 5-dehydrogenase (Mt1D) was over-expressed to study the effect of mannitol over-production in Parachlorella kessleri under high-light induced stress. Over-expression of Mt1D led to 65% increased mannitol content in the transformed P. kessleri compared to that of wild type. Mannitol transformant demonstrated > 20-fold reduction in reactive oxygen species generation and 15% higher biomass productivity when grown in outdoor cultivation with high-light irradiance of 1200 μmol photons m−2 s−1. Conclusions The current study establishes that a higher mannitol concentration provides stress shielding and leads to better acclimatization of transgenic microalgae against high-light generated stress. It also led to reduced ROS generation and improved growth of microalga under study. Thus, overexpression of the Mt1D gene in microalgae can be a suitable strategy to combat high-light stress.

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Section: Methodsmentioning

confidence: 99%

“…The samples were centrifuged, and supernatant was taken to measure the absorbance at 665, 652, and 480 nm. Standard equations were used to calculate the chlorophyll a, chlorophyll b, and total carotenoids [ 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

Heterologous mannitol-1-phosphate dehydrogenase gene over-expression in Parachlorella kessleri for enhanced microalgal biomass productivity

Rathod

Vira

Lali

et al. 2022

Journal of Genetic Engineering and Biotechnology

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“…The Chlamydomonas reinharditii , a known, non β-1,3-glucan producer was used as a control in prebiotic experiments and grown in Tris Acetate Phosphate medium at a pH of 7 ( Kumari et al., 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Prebiotic activity of paramylon isolated from heterotrophically grown Euglena gracilis

Bhattad¹,

Koradiya

Prakash³

2021

Heliyon

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…Similarly, for algae, not only overexpression of the native OR gene or the mutant OR His but also heterologous expression of OR gene from Brassica oleracea into C. reinhardtii can help increase the carotenoid accumulation and resistance to abiotic stress. 110,111 Recently, overexpression of DsOR, especially the mutant DsOR His , also significantly improved the single-cell carotenoid content and changed cell morphology (with larger cell size, bigger plastoglobuli, and fragmented starch granules) in the β-carotene-overproducing D. salina. 112 DsOR protein from D. salina, a good selection for studying the mechanism of carotenoid accumulation and producing natural carotenoids, could interact with DsPSY1/2 and regulate the plastid development.…”

Section: Regulation Of Carotenoid Biosynthesis By Regulators In Plant...mentioning

confidence: 99%

Involvement of Transcription Factors and Regulatory Proteins in the Regulation of Carotenoid Accumulation in Plants and Algae

Liang,

2023

J. Agric. Food Chem.

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Heterologous expression of a mutant Orange gene from Brassica oleracea increases carotenoids and induces phenotypic changes in the microalga Chlamydomonas reinhardtii

Cited by 22 publications

References 33 publications

Heterologous mannitol-1-phosphate dehydrogenase gene over-expression in Parachlorella kessleri for enhanced microalgal biomass productivity

Heterologous mannitol-1-phosphate dehydrogenase gene over-expression in Parachlorella kessleri for enhanced microalgal biomass productivity

Prebiotic activity of paramylon isolated from heterotrophically grown Euglena gracilis

Involvement of Transcription Factors and Regulatory Proteins in the Regulation of Carotenoid Accumulation in Plants and Algae

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