Prebiotic activity of paramylon isolated from heterotrophically grown Euglena gracilis

Bhattad, Tanmay; Koradiya, Akshaykumar; Prakash, Gunjan

doi:10.1016/j.heliyon.2021.e07884

Cited by 12 publications

(12 citation statements)

References 32 publications

(38 reference statements)

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“…In a recent report, Euglena stimulates Faecalibacterium in the human gut and contributes to increased defection ( 12 ). In addition, the prebiotic activity of paramylon isolated from heterotrophic Euglena was reported to lead to cell number enhancement of Lacfid ( Lactobacillus ) ( 13 ). After 36 h cultivation, a significant difference in Lactobacillus growth was obtained by adding Euglena powder and paramylon individually, showing a ∼25% increase in cfu per ml of Lactobacillus compared to that of control ( 13 ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the prebiotic activity of paramylon isolated from heterotrophic Euglena was reported to lead to cell number enhancement of Lacfid ( Lactobacillus ) ( 13 ). After 36 h cultivation, a significant difference in Lactobacillus growth was obtained by adding Euglena powder and paramylon individually, showing a ∼25% increase in cfu per ml of Lactobacillus compared to that of control ( 13 ). This study investigated nutrients from AE and HE based on different culture methods.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, evidence has appeared for the positive health effects of foods, food ingredients, or biochemical compounds derived from several other microalgae, such as Chlorella , Spirulina , Dunaliella , Phaeodactylum , and Pavlova ( 11 ). Until recently, the prebiotic activity of Euglena and paramylon was reported ( 12 , 13 ). For example, Euglena stimulates Faecalibacterium in the human gut and contributes to increased defecation ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

“…For example, Euglena stimulates Faecalibacterium in the human gut and contributes to increased defecation ( 12 ). Heterotrophic Euglena cell extract and isolated paramylon led to the growth promotion of Lacfid ( Lactobacillus ) ( 13 ). However, whether any other substances in Euglena’s potential as prebiotics remain unknown.…”

Section: Introductionmentioning

confidence: 99%

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Euglena gracilis Promotes Lactobacillus Growth and Antioxidants Accumulation as a Potential Next-Generation Prebiotic

Dai

He²,

Chen³

et al. 2022

Front. Nutr.

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Euglena gracilis, a single-celled microalga with various trophic growth styles under different cultivation conditions, contains nutrients, such as ß-1,3-glucans, essential amino acids, fatty acids, vitamins, and minerals. It has recently attracted attention as a new health food. Among them, ß-1,3-glucans, paramylon of Euglena, is an insoluble dietary fiber and is well known as an immune booster, attenuator of obesity and diabetes, reducer of acute liver injury, and suppressor of atopic dermatitis, and other chronic inflammatory disorders. Recently, evidence has appeared for the positive health effects of foods, food ingredients, or biochemical compounds derived from several other microalgae, such as Chlorella, Spirulina, Dunaliella, Phaeodactylum, and Pavlova. Until most recently, the prebiotic activity of Euglena and paramylon was reported. Emerging prospects of microalgae as prebiotics were well summarized, but the mechanisms behind the bacterial growth promotion by microalgae are not elucidated yet. Thus, we evaluated the prebiotic prospects of both autotrophic and heterotrophic Euglena on six different Lactobacillus. What’s more, the stimulated mechanism was revealed by bacterial culture medium metabolomic analysis. This study could widen the knowledge about the prebiotic activity of Euglena as a next-generation prebiotic and other microalgae-derived compounds as potential health foods.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Euglena gracilis Promotes Lactobacillus Growth and Antioxidants Accumulation as a Potential Next-Generation Prebiotic

Dai

He²,

Chen³

et al. 2022

Front. Nutr.

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“…The Euglena gracilis powder was refluxed with petroleum ether (1: 10, w / v ) in an automatic Soxhlet extractor (Naai, Shanghai, China) for 3 h, and air-dried to obtain defatted and decolorized Euglena gracilis powder. In accordance with the previous method [ 17 ], Euglena gracilis powder was suspended in 10 g/L sodium dodecyl sulfate (SDS), mixed evenly. This mixture was allowed to react in a water bath at 50 °C for 2 h, and centrifuged for 10 min at 6000 rpm.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Antibacterial Activities of Carboxymethylated Paramylon from Euglena gracilis

Gao

Zhao

et al. 2022

Polymers

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Paramylon from Euglena gracilis (EGP) is a polymeric polysaccharide composed of linear β-1,3 glucan. EGP has been proved to have antibacterial activity, but its effect is weak due to its water insolubility and high crystallinity. In order to change this deficiency, this experiment carried out carboxymethylated modification of EGP. Three carboxymethylated derivatives, C-EGP1, C-EGP2, and C-EGP3, with a degree of substitution (DS) of 0.14, 0.55, and 0.78, respectively, were synthesized by varying reaction conditions, such as the mass of chloroacetic acid and temperature. Fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), and nuclear magnetic resonance (NMR) analysis confirmed the success of the carboxymethylated modification. The Congo red (CR) experiment, scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetry (TG) were used to study the conformation, surface morphology, crystalline nature, and thermostability of the carboxymethylated EGP. The results showed that carboxymethylation did not change the triple helix structure of the EGP, but that the fundamental particles’ surface morphology was destroyed, and the crystallization area and thermal stability decreased obviously. In addition, the water solubility test and antibacterial experiment showed that the water solubility and antibacterial activity of the EGP after carboxymethylation were obviously improved, and that the water solubility of C-EGP1, C-EGP2, and C-EGP3 increased by 53.31%, 75.52%, and 80.96% respectively. The antibacterial test indicated that C-EGP3 had the best effect on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with minimum inhibitory concentration (MIC) values of 12.50 mg/mL and 6.25 mg/mL. The diameters of the inhibition zone of C-EGP3 on E. coli and S. aureus were 11.24 ± 0.15 mm and 12.05 ± 0.09 mm, and the antibacterial rate increased by 41.33% and 43.67%.

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Bottom‐Up Synthesized Glucan Materials: Opportunities from Applied Biocatalysis

Zhong,

Nidetzky

2024

Advanced Materials

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Linear D‐glucans are natural polysaccharides of simple chemical structure. They are comprised of D‐glucosyl units linked by a single type of glycosidic bond. Noncovalent interactions within, and between, the D‐glucan chains give rise to a broad variety of macromolecular nanostructures that can assemble into crystalline‐organized materials of tunable morphology. Structure design and functionalization of D‐glucans for diverse material applications largely relies on top‐down processing and chemical derivatization of naturally derived starting materials. The top‐down approach encounters critical limitations in efficiency, selectivity, and flexibility. Bottom‐up approaches of D‐glucan synthesis offer different, and often more precise, ways of polymer structure control and provide means of functional diversification widely inaccessible to top‐down routes of polysaccharide material processing. Here we describe the natural and engineered enzymes (glycosyltransferases, glycoside hydrolases and phosphorylases, glycosynthases) for D‐glucan polymerization and show the use of applied biocatalysis for the bottom‐up assembly of specific D‐glucan structures. We further show advanced material applications of the resulting polymeric products and discuss their important role in the development of sustainable macromolecular materials in a bio‐based circular economy.This article is protected by copyright. All rights reserved

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Prebiotic activity of paramylon isolated from heterotrophically grown Euglena gracilis

Cited by 12 publications

References 32 publications

Euglena gracilis Promotes Lactobacillus Growth and Antioxidants Accumulation as a Potential Next-Generation Prebiotic

Euglena gracilis Promotes Lactobacillus Growth and Antioxidants Accumulation as a Potential Next-Generation Prebiotic

Characterization and Antibacterial Activities of Carboxymethylated Paramylon from Euglena gracilis

Bottom‐Up Synthesized Glucan Materials: Opportunities from Applied Biocatalysis

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