Heterologous antibodies to evaluate the kinetics of the humoral immune response in dogs experimentally infected with Toxoplasma gondii RH strain

Silva, Deise A. O.; Silva, Neide M.; Mineo, Tiago Wilson Patriarca; Neto, Adalberto Albuquerque Pajuaba; Ferro, Eloísa Amália Vieira

doi:10.1016/s0304-4017(02)00132-2

Cited by 50 publications

(33 citation statements)

References 21 publications

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“…Since the antibodies were related to chronic infection, the IgG class had the capacity of being active even after later detection (up to the 84 th DPI in this study) when compared with immunoglobulin from the IgM class. This class can only be identified up to a few weeks after infection (Silva et al 2002).…”

Section: Resul Resul Resul Resul Results and Discus Ts And Discus Ts mentioning

confidence: 99%

Toxoplasma gondii in semen of experimentally infected swine

Moura¹,

Costa²,

Filho³

et al. 2007

Pesq. Vet. Bras.

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Section: Resul Resul Resul Resul Results and Discus Ts And Discus Ts mentioning

confidence: 99%

Toxoplasma gondii in semen of experimentally infected swine

Moura¹,

Costa²,

Filho³

et al. 2007

Pesq. Vet. Bras.

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“…The cutoff of the reaction was determined as the mean OD of negative control sera plus 3 standard deviations. Antibody titers were arbitrarily expressed as ELISA index (EI) values, according to the formula OD sample /OD cutoff , as described previously (28). Samples with EI values of Ͼ1.2 were considered positive, and borderline EI reactivity values close to 1.0 were excluded.…”

Section: Methodsmentioning

confidence: 99%

Reverse Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibodies against SAG1-Related Sequence, SAG2A, and p97 Antigens fromToxoplasma gondiiTo Detect Specific Immunoglobulin G (IgG), IgM, and IgA Antibodies in Human Sera

Carvalho

Silva

Cunha-Júnior

et al. 2008

Clin Vaccine Immunol

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The present study aimed to evaluate the performance of three monoclonal antibodies (MAbs) in reverse enzyme-linked immunosorbent assays (ELISAs) for detecting immunoglobulin G (IgG), IgM, and IgA antibodies against Toxoplasma gondii in 175 serum samples from patients at different stages of T. gondii infection, as defined by both serological and clinical criteria, as follows: recent (n ‫؍‬ 45), transient (n ‫؍‬ 40), and chronic (n ‫؍‬ 55) infection as well as seronegative subjects (n ‫؍‬ 35). The results were compared with those obtained by indirect ELISA using soluble Toxoplasma total antigen (STAg). Our data demonstrated that MAb A3A4 recognizes a conformational epitope in SAG1-related-sequence (SRS) antigens, while A4D12 and 1B8 recognize linear epitopes defined as SAG2A surface antigen and p97 cytoplasmatic antigen, respectively. Reverse ELISA for IgG with A3A4 or A4D12 MAbs was highly correlated with indirect ELISA for anti-STAg IgG, whereas only A4D12 reverse ELISA showed high correlation with indirect ELISA for IgM and IgA isotypes. To our knowledge, this is the first report analyzing the performance of a reverse ELISA for simultaneous detection of IgG, IgM, and IgA isotypes active toward native SAG2A, SRS, and p97 molecules from STAg, using a panel of human sera from patients with recent and chronic toxoplasmosis. Thus, reverse ELISA based on the capture of native SAG2A and SRS antigens of STAg by MAbs could be an additional approach for strengthening the helpfulness of serological tests assessing the stage of infection, particularly in combination with highly sensitive and specific assays that are frequently used nowadays for diagnosis of toxoplasmosis during pregnancy or congenital infection in newborns.

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“…All measurements were performed in duplicate. the results are expressed as an elISA Index (eI), where eI = ODsample/ODcontrol, and values of eI >1.2 were considered positive (Silva et al, 2002).…”

Section: Animalsmentioning

confidence: 99%