Toxoplasma gondii in semen of experimentally infected swine

Moura, Anderson Barbosa de; Costa, Alvimar José da; Filho, Sérgio Jordão; Paim, Beatriz Bassora; Pinto, F. R.; Mauro, Daniela Cristina Di

doi:10.1590/s0100-736x2007001000008

Cited by 39 publications

(27 citation statements)

References 18 publications

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“…Total Bioprova 3 3 3 5 2 4 4 3 2 2 31 Total PCR 2 1 0 0 1 2 2 0 0 0 8 6 a I I Experimental infection triggered a rapid immunological response, with antibody detection occurring from day 5 PI (Table 2). This early humoral immune response in experimental infections of T. gondii was also detected by Berverley & Waston (1971) in sheep and by Moura et al (2007) in swine. In this study, antibody were firstly detected on day 5 PI and remained at high levels from 7 to 35 DPI, when a gradual reduction in humoral immune response occurred; however, none of the steers were sero-negative by the end of the experimental period.…”

Section: Resultssupporting

confidence: 76%

“…Although the isolation of T. gondii in the semen of sheep (Spence et al, 1978), goats (Dubey & Sharma, 1980) and swine (Moura et al, 2007) has been successfully realized, there are no studies in the literature that prove the isolation of this protozoan in the semen of cattle presenting toxoplasmic infection. The present study aimed to diagnose the eventual presence of T. gondii in semen samples of experimentally infected steers.…”

Section: Introductionmentioning

confidence: 99%

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Toxoplasma gondii in experimentally infected Bos taurus and Bos indicus semen and tissues

et al. 2009

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RESUMO.-[Toxoplasma gondii em semen e tecidos de Bos taurus and Bos indicus experimentalmente infectados.] Dezoito bovinos foram inoculados comToxoplasma gondii e distribuídos aleatoriamente em três grupos de seis bovinos cada: GI (2,5x10 5 oocistos da cepa "P"), GII (5,0x10 6 taquizoítos da cepa "RH") e GIII (controle). Exames clínicos, sorológicos e parasitêmicos foram realizados. Pesquisas do parasito, por meio da bioprova e pela técnica de Reação em Cadeia pela Polimerase (PCR), foram realizadas no sêmen e em fragmentos de musculatura esquelética, linfonodos, cérebro, retina, baço, fígado, pulmão, testículo, epidídimo e vesícula seminal. Amostras de sangue e sêmen foram colhidas nos dias -2, -1, 1, 3, 5, 7, 14 e, semanalmente, até o 84º dia pós-infecção (DPI). Os bovinos inoculados (GI e GII) apresentaram hipertermia do 3º ao 16º DPI. Anticorpos contra T. gondii foram detectados (IFI) no 5º DPI (1:16), em ambos grupos inoculados (oocistos e taquizoítos), atingindo picos de 1:4096 no 7º DPI. Surtos parasitêmicos ocorreram em todos os bovinos infectados, principalmente do 7º ao 28º DPI, independente da cepa e inóculo utilizados. O bioensaio revelou a presença do parasito em amostras seminais dos bovinos infectados com oocistos (GI) e taquizoítos (GII), em diversas datas experimentais, entre o 7º e 84º DPI. Parasitismo tissular por T. gondii foi diag- Eighteen young steers were inoculated with Toxoplasma gondii and randomly distributed into three groups of six animals each: GI, 2.5x10 5 "P" strain oocysts, GII, 5.0x10 6 "RH" strain tachyzoites, and GIII (Control). Clinical, serological and parasitemia exams were realized. Parasite investigation by bioassay and PCR was realized on semen and fragments of skeletal musculature, lymph nodes, brain, retina, spleen, liver, lung, testicle, epididymis and seminal vesicle. Blood and semen samples were collected on days -2, -1, 1, 3, 5, 7, 14 and weekly thereafter, up to postinfection day (PID) 84. The inoculated steers (GI and GII) presented hyperthermia from PID 3 to 16. Antibodies against T. gondii were detected through the indirect fluorescence antibody test (IFAT) on PID 5 (1:16) in both inoculated groups (oocysts and tachyzoites), reaching peaks of 1:4096 on PID 7. Parasitemia outbursts occurred in all infected bovines, principally from PID 7 to 28, independent of the strain and inoculate used. Bioassays revealed the presence of parasites in semen samples of animals infected with oocysts (GI) and tachyzoites (GII) on several experimental days between PID 7 and 84. Tissue parasitism by T. gondii was diagnosed by bioassay and the PCR technique in several organ and tissue fragments. These findings suggest the possibility of sexual transmission of T. gondii in the bovine species. nosticado por meio da bioprova e pela técnica da PCR, em vários fragmentos de tecidos e/ou órgãos. Os achados sugerem a possibilidade da ocorrência da transmissão sexual do T. gondii na espécie bovina.

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Section: Resultssupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Toxoplasma gondii in experimentally infected Bos taurus and Bos indicus semen and tissues

et al. 2009

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“…This was agreed by Scarpelli et al [10] who revealed the existence of the parasite in semen samples of animals infected with oocysts (GI) and tachyzoites (GII) which suggest the opportunity of sexual transmission of T. gondii in the bovine species. This also was supported by the isolation of T. gondii in the semen of sheep [9], goats [24] and swine [25]. Moreover, the T.…”

Section: Discussionmentioning

confidence: 69%

Evaluation of Buffalo Bull Semen for Some Venereal Diseases Using PCR

Abdel-hameed¹,

Mahmoud²,

Sakr

et al. 2017

NIDOC-ASRT

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A RTIFICIAL INSEMINATION using unaccredited frozen semen is a major approach for diseases transmission. This work intended to scan the buffalo bull semen for Br. melitensis, Toxoplasma gondii and Neospora caninum using Polymerase Chain Reaction (PCR). Fifty bulls were used in this study. Semen was assessed for volume, motility, viability, and chromatin damage. Total genomic DNA was extracted from buffalo semen and amplified by PCR. The results showed that, semen samples were positive to Toxoplasma gondii in 6% of the semen samples, while all the semen samples were totally negative to Br. Melitensis and Neospora caninum. No significant differences were found in semen characteristics between bulls positive or negative to Toxoplasma gondii.In conclusion, the present trial suggests the opportunity of venereal transmission of T. gondii among buffaloes. However, further investigations are required using high number of semen samples from different localities of Egypt.

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“…Although the presence of DNA of the parasite does not indicate the viability of the agent nor its potential infectivity, Moura et al (2007) observed that PCR have advantages over traditional methods (bioassay) and research shows a good correlation between PCR and bioassay (Homan et al, 2000;Dehkordi et al, 2013. ).…”

Section: Resultsmentioning

confidence: 99%

Toxoplasma gondii in milk of naturally infected dairy ewes on west mesoregion of Santa Catarina state, Brazil

Ossani

Borges

Souza

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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This study aimed to detect Toxoplasma gondii in the milk of dairy sheep in the Western mesorregion of state of Santa Catarina by bioassay (22 milk samples from eight ewes seropositive; IFA ≥256) and PCR [for the detection of agent in the brains of mice inoculated on bioassay and directly from milk (108 samples from 42 seropositive ewes (IFA, ≥64) in different lactation periods)]. T. gondii DNA was detected in mice brains inoculated with milk from eight sheep (a sample of the 45th day of lactation and seven in the collection of 90th day) and directly from the milk in samples of the second collection (90 days) in five animals. Taking into account both assays, from a total of 42 ewes in lactation and seropositive for T. gondii, 30.95% (13/42) of the animals presented evidences of T. gondii presence in milk. Positive PCR samples were sequenced and the results confirmed ≥97% identity with the membrane antigen P22 gene of T. gondii. The results showed that T. gondii is present in the milk of sheep, representing a possible source of infection to humans through the consumption of milk "in natura" and/or derivatives, besides the possibility of lactogenic transmission to lambs.

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Toxoplasma gondii in semen of experimentally infected swine

Cited by 39 publications

References 18 publications

Toxoplasma gondii in experimentally infected Bos taurus and Bos indicus semen and tissues

Toxoplasma gondii in experimentally infected Bos taurus and Bos indicus semen and tissues

Evaluation of Buffalo Bull Semen for Some Venereal Diseases Using PCR

Toxoplasma gondii in milk of naturally infected dairy ewes on west mesoregion of Santa Catarina state, Brazil

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