Heterogeneity ofHLA defined in PLT: A cellular assay detects differences not seen using HLA-DRw serology

The HLA-D region encodes for several serologically defined systems, including DR, MB, and MT. The antigens of MB and MT are strongly associated with two or more DR specificities. The purpose of this study was to determine the role of MB and MT antigens in lymphocyte alloactivation. A soft agar colony assay was used to generate alloreactive lymphocyte clones primed in mixed leukocyte culture against a stimulator who typed as HLA-DR4,-;MB3,-;MT3,-. In secondary primed lymphocyte typing (PLT) assays, several clones were identified with PLT specificities strongly associated with DR4, MB3, or MT3. The data suggest that HLA-D controls different lymphocyte-activating determinants associated with the serologically defined DR, MB, or MT antigens.

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Association of PLT specificity of alloreactive lymphocyte clones with HLA-DR, MB and MT determinants

Zeevi

Scheffel

Annen

et al. 1982

Immunogenetics

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The HLA-D system: At least two loci and four distinct phenotypic traits per haplotype. Introduction to component typing in families and population by primed lymphocyte typing

et al. 1981

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Using a number of intrafamilial PLTs raised against identical HLA haplotypes it has been possible to construct a model in an informative family defining the HLA-D region as a genetic system. This system consists of at least two regions separated by a recombination between HLA-D and GLO. In relation to the site of recombination, a minimum of one centromeric and three telomeric components can be identified per haplotype. - Fourteen PLTs raised and defined within the family were subsequently tested in a Caucasian population (n = 84) and in 13 unrelated, complete families. - It is concluded that the hypothetical model proposed for the HLA-D regions as a genetic system of linked loci, coding at the cell surface for associated but distinct components (at least four per haplotype), allows for typing of the components of the HLA-D system of any given haplotype. Serological typing of HLA-D components should, in the near future, provide a more convenient way of establishing component phenotypes than the present use of primed lymphocyte typing reagents. Among the components isolated, some have a high association with the classic alleles defined either by homozygous typing cells or DR serology. Others form the basis of cross-reactivity but their presence does not interfere with standard typing. Others, however, seem by their mere presence to be responsible for false assignments. - The concept of HLA-D as a genetic system clarifies many of the inconsistencies observed with a one-locus system.

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Immunogenetic analysis of the HLA-D region: Serological and cellular detection of the MB system

Duquesnoy

Zeevi

Marrari

et al. 1982

Clinical Immunology and Immunopathology

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Heterogeneity ofHLA defined in PLT: A cellular assay detects differences not seen using HLA-DRw serology

Cited by 6 publications

References 19 publications

Association of PLT specificity of alloreactive lymphocyte clones with HLA-DR, MB and MT determinants

Association of PLT specificity of alloreactive lymphocyte clones with HLA-DR, MB and MT determinants

The HLA-D system: At least two loci and four distinct phenotypic traits per haplotype. Introduction to component typing in families and population by primed lymphocyte typing

Immunogenetic analysis of the HLA-D region: Serological and cellular detection of the MB system

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