Immunogenetic analysis of the HLA-D region: Serological and cellular detection of the MB system

Duquesnoy, René J.; Zeevi, Adriana; Marrari, Marilyn; Halim, Kamal

doi:10.1016/0090-1229(82)90112-x

Cited by 22 publications

(4 citation statements)

References 26 publications

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“…In our study, the determinants we have been dealing with were detected also on B activated lymphocytes and did not segregate with HLA. Guilherme et al (1983) have also shown the presence of antigens appearing after activation on T lymphocytes which can be detected on resting B lymphocytes, corresponding to the "MB" series (Duquesnoy et al 1982). Within families this series segregated with the HLA-D part of chromosome 6.…”

Section: Discussionmentioning

confidence: 95%

Non HLA antigenic determinants expressed on activated T and B human lymphocytes

et al. 1984

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Human antigenic determinants present on activated T and B lymphocytes and not on resting B and T cells defined by serological studies are reported. The reactivity of 13 sera against human PBM activated by various agents was tested using microlymphocytotoxicity technique. Results showed that generally similar reactions are present on B and T blasts whatever the technique of activation (PHA, ConA, PWM, TCGF, EBV and allo activation). Absorption elution studies with 5 sera on 10 alloactivated cells clearly demonstrated their independence from DR determinants. The analysis of results obtained by these 15 sera on blasts from 50 donors showed no correlation with HLA-A, -B, -C, DR and -MB antigens, neither can it be HT (Qa-like) antigens. Furthermore, 5 clusters of sera are reported. The weak correlation coefficients cannot affirm the existence of a system with an allelic distribution although each cell except one out of a panel of 50 individuals carries at most 2 specificities. The segregation of 3, of these specificities within 5 informative familics showed no linkage with HLA genes.

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Section: Discussionmentioning

confidence: 95%

Non HLA antigenic determinants expressed on activated T and B human lymphocytes

et al. 1984

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“…The analysis with normal lymphocytes as secondary MLC stimulators was too limited to demonstrate a clear correlation with HLA type, but the fact that an 'anti-DR 4' PL and the anti-E72 PL were reactive in reciprocal PLT assays, and the selective response of E72 PL to DR4 ÷ and DR7 ÷ lymphocytes is worth recording. Though the central role of DR in PLT has been defined [7], recent studies have also indicated the partidpation of other HLA class-II molecules in PLT [8,20]. Zeevi et al [28] have reported that cloned PL recognise MT3, a class-II supertypic specificity associated with DR4 and 7 [18].…”

Section: Discussionmentioning

confidence: 99%

An HLA-DR negative acute leukaemia which stimulates MLC and CMC responses

Taylor

Fergusson

Dyer

et al. 1983

Cancer Immunol Immunother

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We describe an acute myelomonocytic leukaemia (E72) devoid of cell-surface HLA-DR antigens, but capable of inducing cellular responses. Leukaemia E72 induced proliferation of normal lymphocytes in primary mixed lymphocyte culture (MLC), which was only weakly inhibited by anti-DR sera. Depletion of a small percentage (congruent to 4%) of DR+ cells on a cell-sorter failed to abrogate the capacity of E72 to stimulate MLC and cell-mediated cytotoxicity (CMC) responses. We found that normal lymphocytes primed with E72 responded in secondary MLC to lymphocytes and leukaemias, suggesting that the lymphocyte-activating determinant (LAD) on E72 is not leukaemia-specific. In addition, E72 induced CMC responses to both leukaemias and lymphocytes. We suggest that E72 may express a novel HLA or non-HLA LAD.

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“…However, multiple lines of evidence indicate that there is at least one other, and perhaps many more, human Ia-like gene products. This complexity is evident from serologic (6)(7)(8)(9) and immunochemical (10)(11)(12)(13)(14)(15)(16)(17)(18)(19) analyses; however, little is known about the ability of these determinants to function in T cell recognition (20), and the available genetic data (7,21) are insufficient to conclusively establish that these determinants are controlled by genes distinct from HLA-DR. Such correlations between structure, genetics, and function will have to be established to achieve a more general understanding of human Ia antigens.…”

mentioning

confidence: 99%

Serologic identification of the human secondary B cell antigens. Correlations between function, genetics, and structure.

Shaw

DeMars

Schlossman

et al. 1982

The Journal of Experimental Medicine

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The secondary B cell (SB) antigens are polymorphic HLA-linked antigens on human B cells and macrophages that are identified by primed T cell responses but are genetically distinct from the HLA-DR, MB, and MT antigens. Serologic identification of the SB molecule, using the monoclonal antibody ILR1, now makes it possible to correlate the function of these determinants in human T cell recognition with an Ia-like molecular structure and a genetic locus that marks a new HLA subregion. Three lines of evidence indicate that the ILR1 molecule identifies an epitope on some alleles of the SB gene: (a) the polymorphism of ILR1 -reactivity in the population correlates with SB2 SB3; (b) T cell proliferative response to SB2 and SB3 are specifically inhibited by ILR1; and (c) ILR1 reactivity is exactly concordant with the expression of SB2 in a panel of HLA-deletion mutant lymphoblastoid cell line. Together with previous studies, these results indicate that the SB antigens are on Ia-like molecules. Furthermore, the serologic studies of HLA-deletion mutant cell lines demonstrate that there are two HLA regions centromeric to HLA-B controlling expression of Ia-like molecules: a region toward HLA-B that controls expression of HLA-DR, and a region toward GLO that controls expression of SB.

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Immunogenetic analysis of the HLA-D region: Serological and cellular detection of the MB system

Cited by 22 publications

References 26 publications

Non HLA antigenic determinants expressed on activated T and B human lymphocytes

Non HLA antigenic determinants expressed on activated T and B human lymphocytes

An HLA-DR negative acute leukaemia which stimulates MLC and CMC responses

Serologic identification of the human secondary B cell antigens. Correlations between function, genetics, and structure.

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