Herbicide Resistance Endowed by Enhanced Rates of Herbicide Metabolism in Wild Oat (<i>Avena</i>spp.)

Ahmad-Hamdani, Muhammad Saiful; Yu, Qin; Han, Heping; Cawthray, Gregory R.; Wang, Shao F.; Powles, Stephen B.

doi:10.1614/ws-d-12-00078.1

Cited by 40 publications

(35 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was in accordance with former researches that NTSR often exists in populations containing target-site based resistance (Ahmad-Hamdani et al, 2012;Kaundun et al, 2013). Given the complexity of NTSR, further researches need to be done to investigate the NTSR in shepherd's purse.…”

Section: Discussionsupporting

confidence: 90%

Herbicides cross resistance of a tribenuron-methyl resistant Capsella bursa-pastoris (L.) Medik. population in wheat field

Zhang

Wei

Guo

et al. 2017

Chilean J. Agric. Res.

View full text Add to dashboard Cite

Shepherd's purse (Capsella bursa-pastoris [L.] Medik.) is a troublesome dicot weed that occurs in major wheat (Triticum aestivum L.) production areas in China. Tribenuron-methyl failed to control shepherd's purse in some fields in Runan County, Henan Province. This study aimed to establish the cross-resistance pattern of a resistant (R) population and explore the potential targetsite and non-target-site based resistance mechanisms. Acetohydroxyacid synthase (AHAS) gene sequencing revealed a single nucleotide change of CCT to CGT resulting in the Pro to Arg substitution at amino acid position 197 in resistant individuals. Compared with the susceptible (S) population, R population displayed high level resistance to tribenuron-methyl. Cross resistance patterns showed that the R population was high resistant to flucarbazone-Na; moderately resistant to florasulam; low resistant to pyrithiobac sodium and pyroxsulam, and sensitive to imazethapyr. The pretreatment of malathion reduced the 50% growth reduction (GR 50 ) value of tribenuron-methyl by 29% in the R population, suggesting that target-site resistance and non-target-site resistance mechanisms were both present in tribenuronmethyl -resistance of shepherd's purse. This is the first report of the acetolactate synthase Pro197Arg mutation in shepherd's purse.

show abstract

Section: Discussionsupporting

confidence: 90%

Herbicides cross resistance of a tribenuron-methyl resistant Capsella bursa-pastoris (L.) Medik. population in wheat field

Zhang

Wei

Guo

et al. 2017

Chilean J. Agric. Res.

View full text Add to dashboard Cite

show abstract

“…Non-target-site resistance is now increasingly identified as the major resistance mechanism to ACCase herbicides (Délye et al, 2011). Additionally, NTSR often exists in populations containing target-site based resistance (Ahmad-Hamdani et al, 2012;Kaundun et al, 2013). Given the complexity of NTSR, further researches need to be done to investigate the NTSR in A. aequalis.…”

Section: Discussionmentioning

confidence: 99%

Herbicides cross resistance of a multiple resistant short-awn foxtail (Alopecurus aequalis Sobol.) population in wheat field

Guo

Zhang

et al. 2016

Chilean J. Agric. Res.

View full text Add to dashboard Cite

Alopecurus aequalis Sobol. is a common grass weed, which has become increasingly troublesome to control in China wheat fields. One A. aequalis population, collected from Anhui Province China, was suspected to be resistant to fenoxaprop-P-ethyl and mesosulfuron-methyl. This study aimed to establish the cross-resistance pattern using the purified subpopulation and explore the potential targetsite and non-target-site based resistance mechanisms. Sequencing results showed that a single nucleotide change of ATT to AAT was present in acetyl-CoA carboxylase (ACCase) gene of the resistant (R) plants, resulting in an Ile2041Asn amino acid substitution. Besides, another single nucleotide change of CCC to CGC was present in acetolactate synthase (ALS) gene of the R plants, resulting in a Pro197Arg amino acid substitution. The homozygous resistant plants were isolated and the seeds were used in whole-plant herbicide bioassays. Compared with the susceptible (S) population, R population displayed high level resistance to fenoxaprop-P-ethyl and mesosulfuronmethyl. Cross resistance patterns showed that the R population was highly resistant to clodinafop-propargyl, moderately resistant to pyroxsulam and flucarbazoncsodium, lowly resistant to pinoxaden, and susceptible to tralkoxydim, sethoxydim, and isoproturon. The pretreatment of piperonyl butoxide reduced the 50% growth reduction (GR50) value of fenoxaprop-P-ethyl, suggesting that target-site resistance and non-target-site resistance mechanisms were both present in fenoxaprop-P-ethyl-resistance of A. aequalis. This is the first report of ACCase Ile2041Asn and ALS Pro197Arg mutation in A. aequalis.

show abstract

“…These mechanisms of resistance are also characterized by higher rates of herbicide detoxification due to increased glutathione-stransferase, cytochrome P 450 monooxygenase or glycosyltransferase activities (Délye et al, 2013). Nevertheless, both mechanisms affect herbicide efficacy and should be evaluated due to the possibility of their coexistence (Ahmad-Hamdani et al, 2013, Brosnan et al, 2016.…”

Section: Introductionmentioning

confidence: 99%

<b>Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides

et al. 2017

View full text Add to dashboard Cite

ABSTRACT. Acetolactate synthase inhibitors are the main group of herbicides used in winter crops in Southern Brazil where their intensive use has selected for herbicide-resistant biotypes of radish. The resistance affects the efficacy of herbicides, and identifying the resistance mechanism involved is important for defining management strategies. The aim of this study was to elucidate the resistance mechanism of radish biotypes by quantifying the enzyme activity, ALS gene sequencing and evaluating the response of biotypes to iodosulfuron and imazethapyr herbicide application after treatment with a cytochrome P 450 monooxygenase inhibitor. The susceptible (B 1 ) and resistant (B 4 and B 13 ) biotypes were from wheat fields in the Northwest of Rio Grande do Sul State. The results demonstrated that the enzyme affinity for the substrate (K M ) was not affected in biotypes B 4 and B 13 but that the V max of the resistant biotypes was higher than that of biotype B 1 . The resistant biotypes showed no differential metabolic response to iodosulfuron and imazethapyr herbicides when inhibited by malathion and piperonyl butoxide. However, gene sequencing of ALS showed a mutation at position 574, with an amino acid substitution of tryptophan for leucine (Trp-574-Leu) in resistant biotypes.Keywords: Raphanus sativus, mechanism of resistence, ALS enzyme activity, gene mutation, metabolism.Mutação da Trp-574-Leu do gene ALS confere resistência de biótipos de nabo ao herbicida iodosulfurom e imazetapir RESUMO. Os inibidores da acetolactato sintase são o principal grupo de herbicidas usados em culturas de inverno do Sul do Brasil onde seu uso intenso selecionou biótipos resistentes de nabo. A resistência afeta a eficácia dos herbicidas, e a identificação do mecanismo de resistência envolvido é importante na definição de estratégias de manejo. O objetivo deste estudo foi elucidar o mecanismo de resistência em biótipos de nabo através da quantificação da atividade da enzima, sequenciamento do gene ALS e avaliar a resposta dos biótipos à aplicação do herbicida iodosulfurom e imazetapir após tratamento com inibidores do metabolismo da citocromo P 450 monooxigenase. Os biótipos suscetível (B 1 ) e resistentes (B 4 e B 13 ) eram de lavouras de trigo da região Noroeste do Estado do Rio Grande do Sul. Os resultados demonstraram que a afinidade da enzima pelo substrato (K M ) não foi afetada nos biótipos B 4 e B 13 , porém o V max dos biótipos resistentes foi superior quando comparado ao biótipo B 1 . Os biótipos resistentes não apresentaram resposta metabólica diferencial ao herbicida iodosulfurom e imazetapir quando inibidos pelo malathion e butóxido de piperolina. Entranto, o sequenciamento do gene ALS evidenciou mutação na posição 574 com uma substituição do aminoácido triptofano por leucina (Trp-574-Leu) nos biótipos resistentes. Palavras-chave:Raphanus sativus, mecanismo de resistência, atividade da enzima ALS, mutação gênica, metabolismo.

show abstract

Herbicide Resistance Endowed by Enhanced Rates of Herbicide Metabolism in Wild Oat (Avenaspp.)

Cited by 40 publications

References 49 publications

Herbicides cross resistance of a tribenuron-methyl resistant Capsella bursa-pastoris (L.) Medik. population in wheat field

Herbicides cross resistance of a tribenuron-methyl resistant Capsella bursa-pastoris (L.) Medik. population in wheat field

Herbicides cross resistance of a multiple resistant short-awn foxtail (Alopecurus aequalis Sobol.) population in wheat field

<b>Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides

Contact Info

Product

Resources

About