Herbicidal activity of pure compound isolated from rhizosphere inhabiting Aspergillus flavus

Abstract

In the quest for bioactive natural products of fungal origin, Aspergillus flavus was isolated from rhizosphere of Mentha piperita using Potato Dextrose Agar (PDA) and Czapec Yeast Broth (CYB) nutrient media for metabolites production. In total, three different metabolites were purified using HPLC/LCMS and the structures were established using 500 Varian NMR experiments. Further the isolated metabolites in different concentrations (10, 100, 1000 μg/mL) were tested for herbicidal activity using Completely Randomized design (CRD) against the seeds of Silybum marianum and Avena fatua which are major threats to wheat crop in Pakistan. Among the isolated metabolites, one compound was found active against the test weed species whose activity is reported in the present work. The chemical name of the compound is 2-(1, 4-dihydroxybutan-2-yl)-1, 3-dihydroxy-6, 8-dimethoxyanthracene-9, 10(4aH, 9aH)-dione with mass of 388. Results showed that all seeds germinated in control treatment; however, with the metabolite treated, the growth was retarded to different levels in all parts of the weeds. At a dose of 1000 μg/mL of the pure compound, 100% seeds of S. marianum and 60% seeds of A. fatua were inhibited. Interestingly, the pure compound exhibited less inhibition of 10% towards the seeds of common wheat (Triticum aestivum).

Introducing Autochthonous Bacterium and Fungus Composition to Enhance the Phytopathogen-Suppressive Capacity of Composts against Clonostachys rosea, Penicillium solitum and Alternaria alternata In Vitro

“…It was shown that culture extracts of Aspergillus species used as biocontrol agents did not cause a phytotoxic effect. Conversely, Khattak et al (2014) [59] obtained results similar to our findings. Their work showed that extracts of Aspergillus sp.…”

Antioxidant, antimicrobial, and anticancer cells line of Aspergillus flavus ON764430 extracts isolated from Al Mudawara Mountain, El Fayum governorate

“…It is well-known to produce a variety of secondary metabolites that are valuable in therapeutics, including those that have anticancer, antimicrobial, cholesterol-lowering, and immunosuppressive properties [ 22,23]. The genus Aspergillus has the highest innovation index among the fungal kingdom when it comes to the creation of biologically active secondary metabolites [24]. Human infections that can result in death are brought on by the emergence of pathogens that are resistant to multiple drugs.…”

Aspergillus flavus originated pure compound as a potential antibacterial

“…Within rhizosphere, a number of biochemical and physiological interactions takes place between plant roots, soil fauna and other microbial communities [ 24 ]. Available data suggests that in comparison to soil born microbial species, fungal strains from plant rhizosphere provides a better chance for isolation of bioactive metabolites with antimicrobial properties [ 10 , 15 , 24 , 25 ]. The present study deals with samples from soil, rhizosphere and different plant parts.…”

“…Many structurally complex and therapeutically important secondary metabolites have been recovered from fungi in recent years [ 7 , 8 ]. In fungal kingdom, Aspergillus genus has the highest innovation index regarding the development of biologically active secondary metabolites [ 9 , 10 ]. These isolated secondary metabolites display a wide range of biological activity including antifungal, antibacterial, and anticancer [ 11 , 12 ].…”