Hepatoprotective and Antioxidant Effects of Ethanol Extract fromPhellinus merrilliion Carbon Tetrachloride-Induced Liver Damage

Abstract

In the present study, we investigated the hepatoprotective and antioxidant capacities of ethanol extract of Phellinus merrillii (PM) on carbon tetrachloride-induced hepatotoxicity. In high-performance liquid chromatography (HPLC) analysis, the finger print chromatogram of PM was established. Both hispolon and PM showed a similar peak at the retention time of 6 min. This implied that PM did contain the active ingredient of hispolon. Treatment with PM (0.5, 1 and 2 g/kg) prior to the administration of carbon tetrachloride (1.5 ml/kg in olive oil, 20%) significantly prevented the increased serum alanine aminotransferase (s-GOT) and serum aspartate aminotransferase (s-GPT) in a dose-dependent manner. We also found that the incidences of ballooning degeneration, necrosis and portal triaditis were lowered in the group pretreated with PM. Carbon tetrachloride induces up-regulation of antioxidant enzymes, including superoxide dismutase (SOD) (86.6%), catalase (58.8%) and glutathione peroxidase (GPx)(64.7%) in the liver. Pretreatment with PM significantly reduced the all these antioxidant enzyme activities. Therefore, we verified that ethanol extract of PM has the hepatoprotective and antioxidant capacities on rats.

Hispolon Protects against Acute Liver Damage in the Rat by Inhibiting Lipid Peroxidation, Proinflammatory Cytokine, and Oxidative Stress and Downregulating the Expressions of iNOS, COX-2, and MMP-9

“…And we also found that hispolon, a phenolic compound from Phellinus merrillii , exhibited antioxidant activity. Phenolic compounds possess antioxidant, radical scavenging, and antimutagenic and anticarcinogenic properties [21]. …”

“…Many studies have demonstrated that antioxidant enzymes such as SOD, CAT, and GPx represent one protection against oxidative tissue damage [21]. SOD is an effective defense enzyme that converts the dismutation of superoxide anions into hydrogen peroxide (H 2 O 2 ) [22].…”

Detection and confirmation of hispolon in the mushroom Phellinus linteus

“…Many factors such as solvent characteristic, extraction time, temperature, and ratio of sample to solvent have an influence on extraction 11 . In this experiment, we selected ethanol as a solvent followed the work of Chen et al 8 because the crude extract of PL contains effective component in an anti-cancer test. Our extraction results showed that the hispolon contents at 4 h of extraction time with 50%, 80%, and 95% ethanol were 0.0000 mg/g, 0.0025 mg/g, and 0.0147 mg/g of dried mushroom powder, respectively.…”

Ionone Derivatives from the Mycelium of Phellinus linteus and the Inhibitory Effect on Activated Rat Hepatic Stellate Cells

“…Various aromatic hydrocarbons including caffeic acid, hydroxybenzaldehyde, hispidin, hispolon, and inotilone have also been characterized from the mycelium extracts and exhibited significant antioxidant and anti-inflammatory activities [ 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. Recently, several articles reported the hepatoprotective and antihepatotoxic effects of the Phellinus genus [ 28 , 29 , 30 , 31 , 32 ]. In our preliminary study, the ethanol extract of cultured mycelium of P. linteus (PLE) exhibited protective effect against hepatic fibrosis in experimental animals.…”