Detection and confirmation of hispolon in the mushroom Phellinus linteus

Toopmuang, Padungrat; Khamchum, Chutarat; Punsuvon, Vittaya

doi:10.2306/scienceasia1513-1874.2014.40.141

Cited by 9 publications

(7 citation statements)

References 12 publications

(6 reference statements)

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“…In this study, we investigated the effect of various concentrations at different time of a new natural compound extract from PL: hispolon. Hispolon (6‐(3,4‐dihydroxy‐phenyl)‐4‐hydroxy‐hexa‐3,5‐dien‐2‐one; C 12 H 12 O 4 ), a yellow pigment, is isolated from the P. linteus , has hepatoprotective, anti‐inflammatory, antimetastatic, and antiproliferative effects . It has been shown that hispolon exhibits anticancer effects through inhibition of cell growth, induction of cell cycle arrest, and suppression of metastasis in various types of cancer cells .…”

Section: Introductionmentioning

confidence: 99%

Effects of hispolon on glioblastoma cell growth

Arcella

Oliva

Sanchez³

et al. 2017

Environmental Toxicology

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Hispolon is a polyphenolic compound isolated from Phellinus linteus which exhibits antitumor activity. Here, we explored the effects of hispolon on human glioblastoma cells U87MG. Cell viability was examined by MTT assay. Growth was investigated by incubating cells with various concentrations of hispolon (25 and 50 µM) for 24, 48 or 72 h and daily cell count. Cell cycle and apoptosis assay were assessed by flow cytometry. Hispolon decreased cell viability in a dose- and time-dependent manner. The cell cycle distribution showed that hispolon enhanced the accumulation of the cells in G2/M phase. Hispolon decreased the expression of G1-S transition-related protein cyclin D4 but increased the expression of CDK inhibitor p21. Additionally, hispolon enhanced the expression of p53. Moreover, hispolon treatment was effective on U87MG cells in inhibiting cell viability and inducing cell apoptosis. Our results indicate that hispolon inhibits the cell viability, induces G2/M cell cycle arrest and apoptosis in glioblastoma U87MG cells, and p53 should play a role in hispolon-mediated antitumor activity.

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Section: Introductionmentioning

confidence: 99%

Effects of hispolon on glioblastoma cell growth

Arcella

Oliva

Sanchez³

et al. 2017

Environmental Toxicology

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“…The chemopreventive efficacy of curcumin has garnered significant attention, due to its low nonspecific toxicity to normal cells. Hispolon, a yellow phenolic pigment isolated from a mushroom species, Phellinus linteus [4], is a polyphenol structurally similar to curcumin, and has been investigated and found effective for anti-inflammatory [5], antioxidant [6], and anticancer [7,8] properties. The anticancer potential of hispolon is possibly due to its ability to halt the cell cycle in several cancer cells, to induce apoptosis by activation of caspase-3, caspase-8 and caspase-9, to inhibit NF-kB signaling, or to downregulate Akt signaling pathways [9].…”

Section: Introductionmentioning

confidence: 99%

Hispolon Cyclodextrin Complexes and Their Inclusion in Liposomes for Enhanced Delivery in Melanoma Cell Lines

Poudel

Annaji

Wibowo

et al. 2022

IJMS

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Hispolon, a phenolic pigment isolated from the mushroom species Phellinus linteus, has been investigated for anti-inflammatory, antioxidant, and anticancer properties; however, low solubility and poor bioavailability have limited its potential clinical translation. In this study, the inclusion complex of hispolon with sulfobutyl ether cyclodextrin (SBEβCD) was characterized, and the Hispolon-SBEβCD Complex (HSC) was included within the sterically stabilized liposomes (SL) to further investigate its anticancer activity against melanoma cell lines. The HSC-trapped-Liposome (HSC-SL) formulation was investigated for its sustained drug delivery and enhanced cytotoxicity. The inclusion complex in the solid=state was confirmed by a Job’s plot analysis, molecular modeling, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), Proton nuclear magnetic resonance (NMR) spectroscopy, and scanning electron microscopy (SEM). The HSC-SL showed no appreciable deviation in size (<150 nm) and polydispersity index (<0.2) and improved drug encapsulation efficiency (>90%) as compared to control hispolon liposomes. Individually incorporated hispolon and SBEβCD in the liposomes (H-CD-SL) was not significant in loading the drug in the liposomes, compared to HSC-SL, as a substantial amount of free drug was separated during dialysis. The HSC-SL formulation showed a sustained release compared to hispolon liposomes (H-SLs) and Hispolon-SBEβCD liposomes (H-CD-SLs). The anticancer activity on melanoma cell lines (B16BL6) of HSC and HSC-SL was higher than in H-CD-SL and hispolon solution. These findings suggest that HSC inclusion in the HSC-SL liposomes stands out as a potential formulation approach for enhancing drug loading, encapsulation, and chemotherapeutic efficiency of hispolon and similar water insoluble drug molecules.

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“…Hispolon (6-(3,4-dihydroxy-phenyl)-4-hydroxy-hexa-3,5-dien-2-one; C 12 H 12 O 4 ), a yellow pigment and a natural polyphenol, isolated from the Phellinus igniarius and Phellinus linteus, is structurally similar to curcumin, but lacks one aryl moiety (Balaji et al, 2015;Hyang et al, 2015;Toopmuang et al, 2014;Wu et al, 2014). It exhibits protective effect against acute liver damage (Huang et al, 2012), in ammation (Chang et al, 2011), metastasis (Huang et al, 2010), proliferation of cancer cells (Huang et al, 2011), generation of ROS (Ali et al, 1996), estrogenesis in breast cancer (Hyang et al, 2015), (Wang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant studies of hispolon and its derivatives.

Saka¹,

Chitra

Narayanasamy

2022

Preprint

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Hispolon, a polyphenolic yellow pigment isolated from Chinese mushrooms which was synthesized by researchers and its derivatives such as monomethyl ether, pyrazole and monomethyl ether pyrazole were evaluated for their chemical stability in cell culture medium, antioxidant effect in Chinese hamster ovarian (CHO) radioprotection in spleen lymphocytes. The results indicate that pyrazole derivatives were more chemically stable in cell culture medium. Further, pretreatment with hispolon and its derivatives in CHO cells showed significant inhibition in the radiation induced Reactive oxygen species (ROS) by pyrazole derivative of hispolon. Similarly, the treatment in the splenic lymphocytes showed no significant expression of antioxidant genes such as γGCL and HO-1, and also no significant radioprotection in the markers like fragmentation of DNA, and pre G1 gated cells. This may be because of the primary nature of lymphocytes with limited life span. In MMP assay hispolon pyrazole and monomethyl ether showed a significant rise indicates that these compounds are protecting through inhibition of radiation induced mitochondrial dysfunction. In conclusion, in line with the previous studies, the pyrazole derivatives of hispolon and hispolon monomethyl ether shows antioxidant and radioprotection.

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Detection and confirmation of hispolon in the mushroom Phellinus linteus

Cited by 9 publications

References 12 publications

Effects of hispolon on glioblastoma cell growth

Effects of hispolon on glioblastoma cell growth

Hispolon Cyclodextrin Complexes and Their Inclusion in Liposomes for Enhanced Delivery in Melanoma Cell Lines

Antioxidant studies of hispolon and its derivatives.

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