Near infrared reflectance spectroscopy (NIRS) coupled with a geometric study was used to estimate the oil content and fatty acid composition of Pongamia pinnata seed powder and seed oil. Sixty seed powders and seed oils from various sources of P. pinnata in southern Thailand were scanned by NIRS. All samples were analysed for oil content by hexane extraction and fatty acid composition by gas chromatography. Calibration equations were developed for oil content and individual fatty acids (palmitic acid C16:0, stearic acid C18:0, oleic acid C18:1, linoleic acid C18:2, and behenic acid C22:0). The results showed that NIRS was a reliable and accurate technique to estimate oil content from seed powder, as well as the concentrations of oleic, behenic, and linoleic acid in seed oil. Screening by NIRS of P. pinnata seed powders and seed oils represents a rapid, simple, and cost-effective alternative method that may be of great utility for analysing a large number of seed samples for tree breeding and plantations for the biofuel industry.
Phellinus linteus (PL), a mushroom known as krathin phiman in Thai, has been used as a traditional medicine in the treatment of various cancers. Hispolon obtained from PL ethanol extracts is a promising anti-cancer compound. In this study, ethanol concentration and extraction time were varied for a soxhlet extraction. The content of hispolon in a crude extract was determined by high performance liquid chromatography with a UV detector. The presence of hispolon in the crude extract was demonstrated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with daughter ion scanned mode. The results showed that both hispolon and Thai PL have a similar parent ion at m/z 218.91 and daughter ions at m/z 176, 160, 135.
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