Hepatoprotective activity of Leptadenia reticulata stems against carbon tetrachloride-induced hepatotoxicity in rats

Abstract

Objective:To evaluate the hepatoprotective activity of ethanolic and aqueous extract of stems of Leptadenia reticulata (Retz.) Wight. and Arn. in carbon tetrachloride (CCl4)-induced hepatotoxicity in rats.Materials and Methods:The toxicant CCl4 was used to induce hepatotoxicity at a dose of 1.25 ml/kg as 1 : 1 mixture with olive oil. Ethanolic and aqueous extracts of L. reticulata stems were administered in the doses of 250 and 500 mg/kg/day orally for 7 days. Silymarin (50 mg/kg) was used as standard drug. The hepatoprotective effect of these extracts was evaluated by the assessment of biochemical parameters such as serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase, total bilirubin, serum protein, and histopathological studies of the liver.Results:Treatment of animals with ethanolic and aqueous extracts significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver as indicated by lower levels of serum bilirubin and protein as compared with the normal and silymarin-treated groups. Histology of the liver sections confirmed that the extracts prevented hepatic damage induced by CCl4 showing the presence of normal hepatic cords, absence of necrosis, and fatty infiltration.Conclusion:The ethanolic and aqueous extracts of stems of L. reticulata showed significant hepatoprotective activity. The ethanolic extract is more potent in hepatoprotection in CCl4-indiced liver injury model as compared with aqueous extract.

Connecting corporations and communities: Towards a theory of social inclusive open innovation

“…In this paper, we have argued for a framework that reduces transaction costs on supply and demand side and make such intermediation possible that needs of the most disadvantaged people don't remain unmet. The way institutions are designed and monitored, it is becoming more Anti-oxidant (root), ant-inflammatory(root), antipyretic (root) (Manda et al 2009), antianemic (root) (Pingali et al 2015), antidiabetic(fruit), antioxidant (fruit) (Bagali and Jalalpure 2010), hepatoprotective activity (fruit) (Bagali and Jalalpure 2010) 2 Karad Dichanthum annulatum Poaceae Grass Perennial Dysentery and manorrhagia (whole plant) (Nisar et al 2014); fodder (Khan et al 2012) Antiviral (Fraction), antimicrobial (Fraction) and cytotoxic activities (Fraction) (Awad et al 2015) 4 Mahuda (Bhatt et al 2002); hematopoiesis, dysentery, emaciation, dyspnea, burning sensation, and night blindness (Sivarajan and Balachandran 1994) 1960), Hepatoprotective activity (Nema et al 2011) Aerial parts: Antifungal activity , (Kaou et al 2008), Antimalarial activity (Kaou et al 2008) Leave: Antiasthmatic Activity (Baheti and Awati 2013), Anticencerous (Sathiyanarayanan et al 2007), Antiulcer Activity (Bodhanapu and Sreedhar 2011) Whole plant: Antidepressant (Hakim 1964), Cardiovascular activity (Mehrotra et al 2007), Oligospermic treatment (Madaan and Madaan 1985) 6 Mankachu (Faizi et al 1994); anti-cancer activity …”

<em>Leptadenia reticulata</em> (Retz.) Wight (Jivanti): Botanical, Agronomical, Phytochemical, Pharmacological, and Biotechnological Aspects

“…A study by Hewageegana et al, [44] have stated that L. reticulata contains total ash (16.61%), water soluble ash (5.90%), acid insoluble ash (2.80%), dietary fiber (14.23%), protein (35.80%), carbohydrates (23.40%), crude fat (2.80%), iron (0.03%), magnesium (1.50%), and calcium (0.97%). Likewise, carbohydrates, glycosides, flavonoids, tannins, saponins, phytosterols, free catechols, starches, and phenolic compounds were also reported in different solvent extracts of L. reticulata stems by researchers [11,41,45,46]. A flavone occurring as C-glycoside has also been identified in this plant [46].…”

“…Nema et al, [45] investigated the hepatoprotective activity of the stems extracts of L. reticulata on paracetamol-induced hepatic damage in albino rats. The hepatoprotective action of ethanolic extract of L. reticulata was evidenced by a significant reduction in the elevated serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, and alkaline phosphatase level.…”

Phytochemical Evaluation and High-Performance Thin Layer Chromatography Profiling: Sapindus Emarginatus Vahl. And Morinda Pubescens J.E.SM. Barks Extracts

“…It is also used in various diseases as treatments of hepatotoxicity, nephrotoxicity, neurotoxicity, diabetes, cancer, Alzheimer's, etc. [5,[17][18][19][20][21].…”

“…Nowadays, researchers worldwide are engaged in searching for alternative drugs for various diseases such as hepatotoxicity, nephrotoxicity, cardiotoxicity, and neurotoxicity (without side effects), only a few significant and effective agents are available in traditional medicine therapy [5][6][7]. But hindered or difficult the acceptance of the alternative medicines in the developed countries, because the lack of documentation and stringent quality control.…”