Hepatoma‐associated nuclear matrix nonhistone antigens

Kiliańska, Zofia M.; Krajewska, Wanda M.; Xie, Ronglin; Klyszejko-Stefanowicz, L; Chiu, Jen‐Fu

doi:10.1002/jcb.240450313

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J of Cellular Biochemistry

1991

DOI: 10.1002/jcb.240450313

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Hepatoma‐associated nuclear matrix nonhistone antigens

Zofia M. Kiliańska¹,

Wanda M. Krajewska²,

Ronglin Xie³

et al.

Abstract: Polyclonal antibodies generated against a group of high molecular weight nonhistone proteins from Morris hepatoma 7777 were used in immunological studies of hepatoma-associated nonhistone proteins in rat and hamster. We revealed the presence of cross-reactive antigens in rat Morris hepatomas 7777 and 8994, and in hamster Kirkman-Robbins hepatoma, but not in normal rat or hamster livers. These specific nonhistone proteins were found to be preferentially localized in the nuclear matrix of rat Morris hepatoma 777… Show more

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Cited by 3 publications

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Nuclear distribution pattern of tumour-associated nonhistone protein of mol. wt 48,000

Krajewska

Lipińska

Gaczyński

et al. 1992

International Journal of Biochemistry

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Nuclear distribution pattern of tumour-associated nonhistone protein of mol. wt 48,000

Krajewska

Lipińska

Gaczyński

et al. 1992

International Journal of Biochemistry

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Colorectal cancer-associated nuclear antigen

Szymczyk

Jakubík

Krajewska

et al. 2000

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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By using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting assays in the presence of polyclonal antiserum raised against electrophoretically specific polypeptides of colorectal cancer nuclear polypeptides with M(r) of 35-40 kDa, we have identified p36 protein whose expression accompanies tumorigenesis of large intestine. Immunological analysis of 35 nuclear protein preparations has indicated expression of p36 antigen in nine of 11 right-sided (81.8%) and 21 of 24 (87.5%) left-sided colorectal tumor cases, but not in any control tissue samples. In this study, we have identified p36 antigen in two colon tumor cell lines, i.e., SW620 and HT29 as well. Fractionation experiments based on selective extraction of nuclei isolated from cancerous specimens, which enables their separation into chromatin, nuclear matrix and its subfraction, i.e., internal and peripheral matrix have revealed the concentration of this particular antigen in the internal matrix.

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Bidwell¹,

McCabe²,

Rougraff³

et al. 1997

Molecular Biology Reports

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Treatment for osteosarcoma is problematic because there are no prognostic markers. Diagnosis is primarily limited to cytologic grading. Oncogenesis alters cell structure therefore osteoblast tissue matrix proteins (extracellular matrix, cytoskeletal, intermediate filament, and nuclear matrix proteins), components of the cell substructure, are candidates for osteosarcoma markers. Structural proteins of the extracellular matrix, e.g. the collagens, are useful for diagnosis but not for tumors that produce little osteoid. To identify principal cellular tissue matrix proteins that distinguish normal from transformed human osteoblasts, their expression in normal osteoblasts, two osteosarcoma cell lines, and three primary osteosarcoma tumors were compared. The tumors were graded as (i) intermediate, (ii) high, and (iii) high grade recurrent. The 1-D SDS/PAGE profiles of the major components of the nuclear matrix and intermediate filament fractions from normal osteoblasts did not vary with biopsy site, age, or sex of patients. These profiles included known cytoskeletal proteins and OB250, a approximately 250 kD protein(s) observed in the intermediate filament fraction. A loss of protein bands, including OB250, was observed in the osteosarcoma cell lines and tumors. The intermediate and high grade tumors exhibited nearly identical protein profiles including potential tumor-specific proteins and collagen, consistent with the presence of intracellular collagen fibers in osteosarcoma. A microsequence was obtained for OT25, a novel low molecular weight protein observed in osteosarcoma cell lines. Fibrinogen gamma-chain, a protein that mediates cell adhesion was recovered from the high grade recurrent tumor.

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Hepatoma‐associated nuclear matrix nonhistone antigens

Cited by 3 publications

References 45 publications

Nuclear distribution pattern of tumour-associated nonhistone protein of mol. wt 48,000

Nuclear distribution pattern of tumour-associated nonhistone protein of mol. wt 48,000

Colorectal cancer-associated nuclear antigen

Untitled

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