Colorectal cancer-associated nuclear antigen

Szymczyk, Piotr; Jakubík, J; Krajewska, Wanda M.; Duś, Danuta; Berner, J; Kiliańska, Zofia M.

doi:10.1016/s0925-4439(00)00017-x

Cited by 2 publications

(4 citation statements)

References 46 publications

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“…p53 overexpression is found more often in RSCC than in cases of LSCC . Immunological analysis of 35 nuclear protein preparations indicated expression of p36 antigen in nine of 11 right‐sided (81.8%) and 21 of 24 (87.5%) left‐sided colorectal tumor cases, but not in any control tissue samples . Proteomic techniques can yield volumes of biological information that are not easily managed by a single laboratory.…”

Section: Discussionmentioning

confidence: 99%

“…(17,18) Immunological analysis of 35 nuclear protein preparations indicated expression of p36 antigen in nine of 11 right-sided (81.8%) and 21 of 24 (87.5%) left-sided colorectal tumor cases, but not in any control tissue samples. (19) Proteomic techniques can yield volumes of biological information that are not easily managed by a single laboratory. Also, variability stemming from different patient populations and methodological inconsistencies between laboratories may contribute to variation in reported findings.…”

Section: Discussionmentioning

confidence: 99%

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Comparative proteomic study for profiling differentially expressed proteins between Chinese left‐ and right‐sided colon cancers

et al. 2012

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The aim of the present study is to profile differentially expressed protein markers between left-sided colon cancer (LSCC) and right-sided colon cancer (RSCC). Fresh tumor tissue samples from LSCC (n = 7) and RSCC (n = 7) groups were analyzed by two-dimensional electrophoresis coupled with MALDI-TOF-MS, followed by Western blotting. In 50 paraffin embedded samples from each group, levels of four differentially expressed proteins (identified by proteomics analysis) were measured by tissue microarray with immunohistochemistry staining to compare the different protein markers between LSCC and RSCC. Sixteen proteins were found to be differentially expressed between LSCC and RSCC. Ten proteins including HSP-60 and PDIA1 were identified to be highly expressed in LSCC (P < 0.01 or P < 0.05), while the expression of six proteins including EEF1D and HSP-27 were higher in RSCC (P < 0.01 or P < 0.05). Virtually all of the indentified proteins were involved in cellular energy metabolism, protein folding/unfolding, and/or oxidative stress. Human colon tumors at various locations have different proteomic biomarkers. Differentially expressed proteins associated with energy metabolism, protein folding/unfolding and oxidative stress contribute to different tumorigenesis, tumor progression, and prognosis between left-and right-sided colon cancer. (Cancer Sci 2013; 104: 135-141) C olon cancer is one of the most common causes of cancerrelated deaths worldwide. In the United States alone, an estimated 101 340 new cases and 49 380 deaths due to colon cancer were recorded in 2011.(1) The molecular mechanisms of carcinogenesis and colon cancer progression are therefore of major clinical importance.With the splenic flexure as the boundary, the colon is anatomically divided into left and right sides. The left-sided colon includes the splenic flexure, descending and sigmoid colons, while the right side includes the ileocecal junction, ascending and transverse colons. Existing evidence indicates that leftsided colon cancer (LSCC) differs importantly from right-sided colon cancer (RSCC) in terms of epidemiology, risk factors, clinical manifestations, and metastatic characteristics. The prevalence of colon cancer location (i.e. left or right) changes with age in both sexes. Older patients are more likely to have RSCC, which grow larger than left-sided lesions.(2) Also while males have a higher prevalence of tumors, women tend to present with right-sided tumor development.(3) Konopke et al. (4) reported that most liver metastases of the right hemicolon were located in the right lobe, while left colonic carcinomas tended to spread homogeneously to both liver lobes. Right-sided colon cancer (RSCC) also shows fewer adenomatous remnants than LSCC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparative proteomic study for profiling differentially expressed proteins between Chinese left‐ and right‐sided colon cancers

et al. 2012

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“…Most importantly, tumor-specific NM proteins have recently been demonstrated in human cancers [28][29][30].…”

Section: Discussionmentioning

confidence: 99%

“…All samples were then dehydrated via graded alcohol (25,30,50,70,90, and 100%) and propylene oxide and embedded in Araldite resin. Semithin sections (1 mm) were cut, stained with toluidine blue, and examined under light microscope.…”

Section: Electron Microscopymentioning

confidence: 99%

Two-Dimensional Electrophoretic Analysis of Nuclear Matrix Proteins in Human Colon Adenocarcinoma

Toumpanaki

Baltatzis

Gaitanarou

et al. 2009

Ultrastructural Pathology

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The aim of the present study was to observe possible qualitative and quantitative expression differences between nuclear matrix proteins (NMPs) of human colon adenocarcinoma and their mirror biopsies, using the technique of two-dimensional gel electrophoresis, in order to identify the existence of specific NMP fingerprints for colon cancer. Colon tissues were examined ultrastructurally and NMPs were isolated biochemically, by serial extraction of lipids, soluble proteins, DNA, RNA, and intermediate filaments and were separated according to their isoelectric point (pI) and their molecular weight (MW) by high-resolution two-dimensional electrophoresis (2D). By comparing the 2D electropherograms of colon cancer tissues and mirror biopsy tissues we observed qualitative and quantitative expression differences between their NMPs but also a differentiation of NMP composition between the stages of malignancy. Moreover, despite the similarities between mirror biopsy samples, a highlight percentage of exception was observed. Electrophoretic results provided in this study demonstrated that the examined NMPs could be further investigated as potential markers for detection of colorectal cancer in an early stage, for the assessment of the disease progression, as well as useful tools for individual therapy and for preventing a possible recurrence of cancer and metastasis.

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Colorectal cancer-associated nuclear antigen

Cited by 2 publications

References 46 publications

Comparative proteomic study for profiling differentially expressed proteins between Chinese left‐ and right‐sided colon cancers

Comparative proteomic study for profiling differentially expressed proteins between Chinese left‐ and right‐sided colon cancers

Two-Dimensional Electrophoretic Analysis of Nuclear Matrix Proteins in Human Colon Adenocarcinoma

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