Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells

Taléns‐Visconti, Raquel; Bonora, Ana; Jover, Ramiro; Mirabet, Vicente; Carbonell, F; Castell, José V.; Gómez-Lechón, Marı́a José

doi:10.3748/wjg.v12.i36.5834

Cited by 229 publications

(177 citation statements)

References 67 publications

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“…There are many studies using HGF and EGF in the hepatocyte inducing protocol, and the concentrations of HGF and EGF showed wide ranges (Snykers et al 2009). The concentration of HGF ranges from 20 ng/ml to 150 ng/ml (Banas et al 2007;Kazemnejad et al 2008;Talens-Visconti et al 2006;Yamamoto et al 2008), and the concentration of EGF ranges from 10 ng/ml to 50 ng/ml (Kazemnejad et al 2008;Lange et al 2005;Shi et al 2005). Wang et al (2004) induced isolated BMMSCs with HGF in vitro at a concentration of 50 ng/ml, which mimicked the injured liver microenvironment.…”

Section: Discussionmentioning

confidence: 99%

VEGF165 expressing bone marrow mesenchymal stem cells differentiate into hepatocytes under HGF and EGF induction in vitro

Yan

Xiao

et al. 2012

Cytotechnology

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A short half-life and low levels of growth factors in an injured microenvironment necessitates the sustainable delivery of growth factors and stem cells to augment the regeneration of injured tissues. Our aim was to investigate the ability of VEGF 165 expressing bone marrow mesenchymal stem cells (BMMSCs) to differentiate into hepatocytes when cultured with hepatocyte growth factor (HGF) and epidermal growth factor (EGF) in vitro. We isolated, cultured and identified rabbit BMMSCs, then electroporated the BMMSCs with VEGF 165 -pCMV6-AC-GFP plasmid. G418 was used to select transfected cells and the efficiency was up to 70%. The groups were then divided as follows: Group A was electroporated with pCMV6-AC-GFP plasmid ? HGF ? EGF and Group B was electroporated with VEGF 165 -pCMV6-AC-GFP plasmid ?HGF ? EGF. After 14 days, BMMSCs were induced into short spindle and polygonal cells. Alpha-fetoprotein (AFP) was positive and albumin (ALB) was negative in Group A, while both AFP and ALB were positive in group B on day 10. AFP and ALB in both groups were positive on day 20, but the quantity of AFP in group B decreased with prolonged time and was about 43.5% less than group A. The quantity of the ALB gene was increased with prolonged time in both groups. However, there was no significant difference between group A and B on day 10 and 20. Our results demonstrated that VEGF 165 -pCMV6-AC-GFP plasmid modified BMMSCs still had the ability to differentiate into hepatocytes. The VEGF 165 gene promoted BMMSCs to differentiate into hepatocyte-like cells under the induction of HGF and EGF, and reduced the differentiation time. These results have implications for cell therapies.

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Section: Discussionmentioning

confidence: 99%

VEGF165 expressing bone marrow mesenchymal stem cells differentiate into hepatocytes under HGF and EGF induction in vitro

Yan

Xiao

et al. 2012

Cytotechnology

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“…They used HGF, OsM, and DMSO and demonstrated that generated hepatocytes had the ability to uptake LDLs and synthesize urea. Next, the protocol of differentiation of BM-MSCs and UCB-MSCs toward hepatogenic lineage by was also confirmed on AT-MSCs (Talens- Visconti et al, 2006). Our group recently reported hepatogenic differentiation of AT-MSCs from cancer patients' own CD105 ϩ AT-MSCs (Banas et al, 2007).…”

Section: Adipose Tissue Mesenchymal Stem Cells (At-mscs)mentioning

confidence: 84%

“…Such a possibility sidesteps the limits regarding ethical and immunocompatible problems. Currently, attention is being given to adipose tissue (AT) as a source of MSCs for regenerative medicine (Banas et al, 2007;Seo et al, 2005;Talens-Visconti et al, 2006;Zuk et al, 2001Zuk et al, , 2002. From AT, a sufficient number of stem cells for stem cell-based therapy may be obtained without invasiveness or damage to a patient's health.…”

Section: Adult Stem Cellsmentioning

confidence: 99%

Stem cell plasticity: Learning from hepatogenic differentiation strategies

Banas¹,

Yamamoto

Teratani³

et al. 2007

Developmental Dynamics

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Many studies on stem cell plasticity are challenging the concept that stem cells contain an intrinsically predefined, unidirectional differentiation program. This means that the developmental fate of a stem cell is dependent on the general potential of the cell (pre-determined stem cell fate) as well as on microenvironmental cues, such as stimuli from growth factors (stem cell niche). Here, we reviewed reports that examined the hepatocyte differentiation ability of stem cells from two different sources: embryonic stem cells and adult stem cells. All of those stem cells revealed the ability to give rise to hepatocyte-like cells using different induction strategies. However, it is still not clear which of those stem cells would be the best source for hepatocyte replacement or which would be the best protocol. We herein present the current knowledge regarding available protocols and factors used in order to obtain functional hepatocytes from stem cells. Developmental Dynamics 236:3228 -3241, 2007.

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“…It can immunomodulate and protect rat hepatocytes when exposed in ALF serum, along with increased production of albumin and urea. ADSCs can be induced to differentiate into hepatocyte-like cells, and could provide a novel source for hepatic regeneration and support hepatic functions [16,26,27]. However, the process of differentiation requires a certain period of time.…”

Section: Discussionmentioning

confidence: 99%

Maintenance of rat hepatocytes under inflammation by coculture with human orbital fat-derived stem cells

Chen¹,

Zhang

Liu

et al. 2012

Cellular and Molecular Biology Letters

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Abbreviations used: ALF -acute liver failure; ADSCs -adipose tissue-derived stem cells; ALB -albumin; CK32 -connexin 32; CYP3A4 -cytochrome P450 subtype 3A4; IDOindoleamine 2,3-dioxygenase; IFN -interferon; IL -interleukin; GAPDH -glyceraldehyde-3-phosphate dehydrogenase; MSCs -mesenchymal stem cells; OFSCs -orbital fat-derived stem cells; PAS -periodic acid-Schiff; TDO2 -tryptophan 2,3-dioxygenase; TNF -tumor necrotic factor Abstract: Preservation of hepatocyte functions in vitro will undoubtedly help the management of acute liver failure. The coculture system may be able to prevent functional decline of hepatocytes. It has already been shown that hepatocytes, when cocultured with bone marrow mesenchymal stem cells, could undergo long-term culture in vitro without loss of functions. In this study, human orbital fat-derived stem cells were isolated and cocultured with rat hepatocytes. When treated with serum from an acute liver failure patient, rat hepatocyte monoculture showed reduction of cell viability and loss of liverspecific functions. However, rat hepatocytes in the coculture system were still able to secret albumin and synthesize urea. IL-6 was significantly elevated in the coculture of rat hepatocyte with orbital fat-derived stem cells, and it might be the key immunoregulator which protects rat hepatocytes against inflammation. Our data confirmed that orbital fat-derived stem cells, or other adipose tissue-derived stem cells, are an ideal candidate to support rat hepatocyte functions in vitro.

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Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells

Cited by 229 publications

References 67 publications

VEGF165 expressing bone marrow mesenchymal stem cells differentiate into hepatocytes under HGF and EGF induction in vitro

VEGF165 expressing bone marrow mesenchymal stem cells differentiate into hepatocytes under HGF and EGF induction in vitro

Stem cell plasticity: Learning from hepatogenic differentiation strategies

Maintenance of rat hepatocytes under inflammation by coculture with human orbital fat-derived stem cells

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