2012
DOI: 10.1007/s10616-012-9439-0
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Abstract: A short half-life and low levels of growth factors in an injured microenvironment necessitates the sustainable delivery of growth factors and stem cells to augment the regeneration of injured tissues. Our aim was to investigate the ability of VEGF 165 expressing bone marrow mesenchymal stem cells (BMMSCs) to differentiate into hepatocytes when cultured with hepatocyte growth factor (HGF) and epidermal growth factor (EGF) in vitro. We isolated, cultured and identified rabbit BMMSCs, then electroporated the BMMS… Show more

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Cited by 10 publications
(12 citation statements)
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References 59 publications
(59 reference statements)
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“…Compared to the above, the expression of the ectodermspecific gene AFP, which is widely used as a hepatic marker, showed a dose-dependent increase and decreased when the concentration of EGF exceeded 5 μg/mL, which suggests that a low concentration of EGF promotes embryonic stem cell differentiation into hepatic cells, while, toxicity at a high concentration of EGF leada to inhibition. It has been reported that 20-45 ng/mL EGF can be used as an inducer to promote hepatic cell directional differentiation from stem cells [21]. Our results showed the promotion of hepatic differentiation effects of EGF on increased expression of the AFP gene.…”
Section: Discussionsupporting
confidence: 64%
“…Compared to the above, the expression of the ectodermspecific gene AFP, which is widely used as a hepatic marker, showed a dose-dependent increase and decreased when the concentration of EGF exceeded 5 μg/mL, which suggests that a low concentration of EGF promotes embryonic stem cell differentiation into hepatic cells, while, toxicity at a high concentration of EGF leada to inhibition. It has been reported that 20-45 ng/mL EGF can be used as an inducer to promote hepatic cell directional differentiation from stem cells [21]. Our results showed the promotion of hepatic differentiation effects of EGF on increased expression of the AFP gene.…”
Section: Discussionsupporting
confidence: 64%
“…The slight increase in viability after 18 days of cultivation could be because of the regeneration of the hepatic cells, as occurring in vivo (Vodovotz et al, 2012). As the increase in viability is limited to serum-free conditions, it might be related to the presence of HGF, which is known to be a mitogen as well as for playing a role in liver regeneration (Forte et al, 2006;Tan et al, 2012).…”
Section: Discussionmentioning
confidence: 99%
“…However, other medium components, such as growth factors, also play a crucial role in the upkeep of hepatic functions. For example, hepatocyte growth factor (HGF) and epidermal growth factor (EGF) are important for the differentiation of hepatocytes and they can lead to improved liver-specific functions in vitro (Kim et al, 2010;Tan et al, 2012).…”
Section: Introductionmentioning
confidence: 99%
“…In the case of liver regeneration attempts, VEGF was likewise recognized to promote BM-MSC differentiation into hepatocyte-like cells under the specific condition of HGF and EGF stimulation [144]. However, most studies have been focused on attempts to induce hepatic differentiation of engineered MSCs with physiologically committed factors.…”
Section: Engineered Mscs In Liver Regenerationmentioning
confidence: 99%
“…GDF-5 [102][103][104] TIMP-1 [106] Bcl-2 [101] BONE FORMATION BMP-2 [86][109-111] BMP-2/SDF-1β [113] BMP-2/HIF-1α [114] BMP-2/Ang-1 [47] BMP-2/VEGF [116][117][118] BMP-2/miR-148b [142] BMP-4/-6/-7 [125,126] VEGF [119,120] Epo [121] IGF-I [128,129] OGP [127] Leptin [130] PDGF [131] ephrinB2 [132] ITGA2 [133] NELL1 [134] β-catenin [135] HI Cb TA Fo Na LIVER REGENERATION VEGF [144] ATP7B [155] HN HN…”
Section: Cartilage Productionmentioning
confidence: 99%