Maintenance of rat hepatocytes under inflammation by coculture with human orbital fat-derived stem cells

Chen, Xia; Zhang, Shichang; Liu, Tao; Liu, Yong; Wang, Yingjie

doi:10.2478/s11658-012-0004-9

Cited by 10 publications

(7 citation statements)

References 29 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although liver transplantation is considered as an ultimate treatment for various liver diseases, such as acute liver failure and end-stage liver disease (Boudechiche et al 2015), it is actually limited in the clinical practice owing to the severe shortage of donor liver organs. At present, several novel approaches, including hepatocyte transplantation and bioartificial liver support system, are being developed to replace the non-functioning liver of those patients waiting for liver transplantation with the aim of improving survival and preventing severe complications (Chen et al 2012;Hughes et al 2008;Lee et al 2016;Rohn et al 2016;Shi et al 2016;Struecker et al 2014). However, the primary hepatocytes easily lose their phenotype and liver-specific functions in vitro if cells are not cultured under the appropriate conditions (Watanabe et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Co-culture system of hepatocytes and endothelial cells: two in vitro approaches for enhancing liver-specific functions of hepatocytes

et al. 2018

View full text Add to dashboard Cite

Although hepatocyte transplantation and bioartificial liver support system provide new promising opportunities for those patients waiting for liver transplantation, hepatocytes are easily losing liver-specific functions by using the common in vitro cultured methods. The co-culture strategies with mimicking the in vivo microenvironment would facilitate the maintenance of liver-specific functions of hepatocytes. Considering that hepatocytes and endothelial cells (ECs) account for 80-90% of total cell populations in the liver, hepatocytes and ECs were directly co-cultured with hepatic stellate cells (HSCs) or adipose tissue-derived stem cells (ADSCs) at a ratio of 700:150:3 or 14:3:3 in the present study, and the liver-specific functions were carefully analyzed. Our results showed that the two co-culture systems presented the enhanced liver-specific functions through promoting secretion of urea and ALB and increasing the expressions of ALB, CYP3A4 and HNF4α, and the vessel-like structure in the co-culture system consisted of hepatocytes, ECs and ADSCs. Hence, our results suggested that the directly co-culture of hepatocytes and ECs with HSCs or ADSCs could significantly improve liver-specific functions of hepatocytes, and the co-culture system could further promote angiogenesis of ECs at a later stage. Therefore, this study provides potential interesting in vitro strategies for enhancing liver-specific functions of hepatocytes.

show abstract

Section: Introductionmentioning

confidence: 99%

Co-culture system of hepatocytes and endothelial cells: two in vitro approaches for enhancing liver-specific functions of hepatocytes

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Over recent decades, orthotopic liver transplantation (OLT) has continued to be the best therapeutic method for acute liver failure (ALF) and end-stage liver disease. 1 However, many transplant patients still die due to shortage of donor organs, surgical complications, and rejection reactions. 2 At present, several novel approaches, including hepatocyte transplantation and bioartificial liver support system, are being developed to replace the nonfunctioning liver of patients waiting for transplantation, with the aim of improving survival and preventing severe complications.…”

Section: Introductionmentioning

confidence: 99%

“…2 At present, several novel approaches, including hepatocyte transplantation and bioartificial liver support system, are being developed to replace the nonfunctioning liver of patients waiting for transplantation, with the aim of improving survival and preventing severe complications. 1 Bioartificial liver systems (BALs) are considered to be a bridge to connect patients and liver transplantation. BALs are also a potential supportive therapy for patients waiting for tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of hydromechanical and functional properties of diversion-type microcapsule-suspension bioreactor for bioartificial liver

Lü

Zhu

2022

Int J Artif Organs

View full text Add to dashboard Cite

Aim: To evaluate the performance of a diversion-type microcapsulesuspension fluidized bed bioreactor and a choanoid fluidized bed bioreactor as bioartificial liver support systems. Materials and methods: We evaluated the performance between the modified fluidized bed bioreactor based on diversion-type microcapsule suspension (DMFBB) and choanoid fluidized bed bioreactor (CFBB). The fluidization performance, fluidized height, bed expansion, and the mechanical stability and strength of microcapsule were determined. The viability, synthetic, metabolism, and apoptosis of microcapsulated HepLi5 cells were evaluated. Finally, samples were collected for measurement of alanine aminotransferase, total bilirubin, direct bilirubin, and albumin concentrations. Results: Uniform fluidization was established in both DMFBB and CFBB. The bed expansion, shear force, retention rate, swelling rate, and breakage rate of microcapsules differed significantly between two bioreactors over 3 days. The viability of microencapsulated HepLi5 cells and the activities of cytochrome P450 1A2 and 3A4 increased on each day in DMFBB compared to the control. The albumin and urea concentrations in the DMFBB displayed obvious improvements compared to the control. Caspase3/7 activities in the DMFBB decreased compared to those in the CFBB. At 24 h, the alanine aminotransferase concentration in the DMFBB declined significantly compared to the control. The total and direct bilirubin concentrations within plasma perfusion were decreased and albumin was increased in the DMFBB at 24 h than in the CFBB. Conclusion: The DMFBB shows a promising alternative bioreactor for use in bioartificial liver support systems for application of clinical practice.

show abstract

“…It is attractive to develop stem-cell-based therapy or transplanting hepatocytes generated in vitro from stem cells for treatment of liver diseases. Many types of stem cells from different sources have been investigated for hepatic differentiation ability (Banas et al 2007, Chen et al 2012, Yamamoto et al 2003. Embryonic stem cells have enormous differentiation ability, but many limitations including teratoma formation after transplantation, immunogenicity, and ethical issues are arresting their clinical application (Lin et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Hepatogenic differentiation from human adipose-derived stem cells and application for mouse acute liver injury

Guo

Wang

Xiong

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Adipose-derived stem cells (ADSCs) derived from adipose tissue have the capacity to differentiate into endodermal, mesoderm, and ectodermal cell lineages in vitro, which are an ideal engraft in tissue-engineered repair. In this study, human ADSCs were isolated from subcutaneous fat. The markers of ADSCs, CD13, CD71, CD73, CD90, CD105, CD166, CYP3A4, and ALB were detected by immunofluorescence assays. Human ADSCs were cultured in a specific hepatogenesis differentiation medium containing HGF, bFGF, nicotinamide, ITS, and oncostatin M for hepatogenic differentiation. The hepatocyte markers were analyzed using immunofluorescence and real-time PCR after dramatic changes in morphology. Hepatocytes derived from ADSCs or ADSCs were transplanted into the mice of liver injury for observation cells colonization and therapy in liver tissue. The result demonstrated that human ADSCs were positive for the CD13, CD71, CD73, CD90, CD105, and CD166 but negative for hepatocyte markers, ALB and CYP3A4. After hepatogenic differentiation, the hepatocytes were positive for liver special markers, gene expression level showed a time-lapse increase with induction time. Human ADSCs or ADSCs-derived hepatocyte injected into the vein could improve liver function repair and functionally rescue the CCl-treated mice with liver injury, but the ADSCs transplantation was better than ADSCs-derived hepatocyte transplantation. In conclusion, our research shows that a population of hepatocyte can be specifically generated from human ADSCs and that cells may allow for participation in tissue-repair.

show abstract

Maintenance of rat hepatocytes under inflammation by coculture with human orbital fat-derived stem cells

Cited by 10 publications

References 29 publications

Co-culture system of hepatocytes and endothelial cells: two in vitro approaches for enhancing liver-specific functions of hepatocytes

Co-culture system of hepatocytes and endothelial cells: two in vitro approaches for enhancing liver-specific functions of hepatocytes

Evaluation of hydromechanical and functional properties of diversion-type microcapsule-suspension bioreactor for bioartificial liver

Hepatogenic differentiation from human adipose-derived stem cells and application for mouse acute liver injury

Contact Info

Product

Resources

About