Hepatoblastoma Biology Using Isotope Ratio Mass Spectrometry: Utility of a Unique Technique for the Analysis of Oncological Specimens

Taran, Katarzyna; Frączek, Tomasz; Sitkiewicz, Anna; Sikora-Szubert, Anita; Kobos, Józef; Paneth, Piotr

doi:10.5604/17322693.1209211

Cited by 3 publications

(7 citation statements)

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“…The head and neck cancers’ occurrence keeps rising so it is essential to establish if spectrometry, this new possibility of evaluation, can be as helpful as other types of diagnosing malignancies. Some studies have already indicated usefulness of IRMS in case of Wilms’ tumour, hepatoblastoma or rhabdomyosarcoma [ 19 , 22 , 32 ]. Numerous articles prove that, based on neoplasm’s isotopic composition and ratios, it is possible to picture its abnormal metabolism that reflects the course of the disease [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have already indicated usefulness of IRMS in case of Wilms’ tumour, hepatoblastoma or rhabdomyosarcoma [ 19 , 22 , 32 ]. Numerous articles prove that, based on neoplasm’s isotopic composition and ratios, it is possible to picture its abnormal metabolism that reflects the course of the disease [ 19 ]. A new method aimed at improving the process of staging may be a huge step forward in the assessment of the threat and towards winning our battle with the disease before it starts developing in an unstoppable manner…”

Section: Discussionmentioning

confidence: 99%

“…The same observations were made by other scientists. Research confirmed that it was possible not only to identify the malignant tissue but also to establish the critical moment of dissemination of the disease and point out the cases of the most doubtful outcome [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…This method allows to determine the ratio of isotopic composition of elements, which reflects the occurrence of physical or chemical reactions and metabolic processes. Data are used to obtain information on biological material through analysis of organic and non-organic compounds [ 19 ]. The most commonly performed is analysis of the isotopic composition of hydrogen, oxygen, carbon, nitrogen and sulphur—the most common elements occurring in proteins.…”

Section: Introductionmentioning

confidence: 99%

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Characteristic of Oral Squamous Cell Carcinoma Tissues Using Isotope Ratio Mass Spectrometry

Bogusiak

Puch

Mostowski

et al. 2020

JCM

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Overall prognosis for patients with oral squamous cell carcinomas (OSSC) is still unfavourable. However, there is a hope that a novel diagnostic method may establish better cancer biology characteristics. The aim of this study was to evaluate the isotope ratio of nitrogen and carbon in OSSC as compared to margin and healthy tissue. A total of 18 patients with OSSC were included in the study. Specimens collected covered: four tumour, four margin and two healthy oral mucosa samples. The samples underwent further procedures: lyophilization and isotope ratio mass spectrometry. Measurements of the ratio of stable isotopes of nitrogen 15N/14N and carbon 13C/12C were performed. It is noticeable that the highest average nitrogen concentration was observed in tumour 12 ± 0.4% and the lowest in healthy tissues 8 ± 0.9% (p < 0.00001). The highest average carbon content was observed in healthy tissues 57 ± 2.2% and the lowest in tumour 46 ± 1.3% (p < 0.00001). Moreover, values of 15N/14N expressed in delta notation were the highest in healthy tissues 9.84 ± 0.61 and the lowest in tumour 8.92 ± 0.58. Values of 13C/12C tended to be higher in tumour −22.2 ± 0.89 and the lowest in healthy tissues −23.7 ± 1.2. Tumour tissues differ in isotopic composition from tissues taken from margin and healthy tissues taken from distant oral mucosa.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characteristic of Oral Squamous Cell Carcinoma Tissues Using Isotope Ratio Mass Spectrometry

Bogusiak

Puch

Mostowski

et al. 2020

JCM

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“…It may also represent a potential source of data needed for the assessment of the most individual characteristics of cancer and complements those at the microscopic or molecular levels. Recently, a few preliminary studies have examined isotope ratios directly in tumour tissues [9, 10, 11, 12] with promising indications that isotopic composition may facilitate the classification of patients into risk groups [11, 12]. However, these previous studies have not covered all the prognostic parameters appropriate to the cancer type, which constitute the existing base for the choice of treatment protocol and prognosis in individual cases.…”

Section: Introductionmentioning

confidence: 99%

The first investigation of Wilms' tumour atomic structure-nitrogen and carbon isotopic composition as a novel biomarker for the most individual approach in cancer disease

et al. 2016

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The paper describes a novel approach to investigating Wilms' tumour (nephroblastoma) biology at the atomic level. Isotope Ratio Mass Spectrometry (IRMS) was used to directly assess the isotope ratios of nitrogen and carbon in 84 Wilms' tumour tissue samples from 28 cases representing the histological spectrum of nephroblastoma. Marked differences in nitrogen and carbon isotope ratios were found between nephroblastoma histological types and along the course of cancer disease, with a breakout in isotope ratio of the examined elements in tumour tissue found between stages 2 and 3. Different isotopic compositions with regard to nitrogen and carbon content were observed in blastemal Wilms' tumour, with and without focal anaplasia, and in poorly- and well-differentiated epithelial nephroblastoma. This first assessment of nitrogen and carbon isotope ratio reveals the previously unknown part of Wilms' tumour biology and represents a potential novel biomarker, allowing for a highly individual approach to treating cancer. Furthermore, this method of estimating isotopic composition appears to be the most sensitive tool yet for cancer tissue evaluation, and a valuable complement to established cancer study methods with prospective clinical impact.

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13C Natural Isotope Abundance in Urothelium as a New Marker in the Follow-Up of Patients with Bladder Cancer

Madej

Forma

Golberg

et al. 2022

Cancers

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Bladder cancer (BC) is the most common urological malignancy and has a high incidence of recurrence. BC cells alter their nutrient uptake and metabolic pathways in order to continue the production of sufficient levels of ATP and metabolic intermediates for proliferation and survival. Changes in metabolic pathways regarding the rate of the enzymatic reaction and transport lead to differences in the content of natural isotopes (13C, 15N, 34S) between normal and cancerous tissues. The assessment of the stable isotopes of carbon, nitrogen, and sulfur in normal urothelium and bladder cancer samples was performed using Isotope Ratio Mass Spectrometry (IRMS). The natural abundance of 15N and 13C was decreased in bladder cancer samples when compared to normal urothelium. No significant correlation was observed in BC specimens depending on the tumor grade and stage. Samples derived from bladder tumors and normal urothelium had a different pattern of 15N and 13C isotope abundance. Decreased 13C natural isotopes in the normal urothelium of BC patients were significantly associated with a shorter DFS. Our results suggest that isotopic analysis of normal urothelium of BC patients can be used to predict bladder cancer recurrence.

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Hepatoblastoma Biology Using Isotope Ratio Mass Spectrometry: Utility of a Unique Technique for the Analysis of Oncological Specimens

Cited by 3 publications

References 4 publications

Characteristic of Oral Squamous Cell Carcinoma Tissues Using Isotope Ratio Mass Spectrometry

Characteristic of Oral Squamous Cell Carcinoma Tissues Using Isotope Ratio Mass Spectrometry

The first investigation of Wilms' tumour atomic structure-nitrogen and carbon isotopic composition as a novel biomarker for the most individual approach in cancer disease

13C Natural Isotope Abundance in Urothelium as a New Marker in the Follow-Up of Patients with Bladder Cancer

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