13C Natural Isotope Abundance in Urothelium as a New Marker in the Follow-Up of Patients with Bladder Cancer

Madej, Adam; Forma, Ewa; Golberg, Michał; Kamiński, Rafał M.; Paneth, Piotr; Kobos, Józef; Różański, Waldemar; Lipiński, Marek

doi:10.3390/cancers14102423

Cited by 6 publications

(2 citation statements)

References 32 publications

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“…On the basis of the available literature, it can be assumed that changes in the isotopic content of nitrogen and carbon in tissue samples can be used to diagnose and monitor the progression of cancer [5][6][7]. For example, lower levels of 15 N in tumour tissue compared to healthy tissue can indicate decreased cellular protein synthesis in the tumour.…”

Section: Discussionmentioning

confidence: 99%

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Oral Cavity Cancer Tissues Differ in Isotopic Composition Depending on Location and Staging

Bogusiak,

Kozakiewicz,

Puch

et al. 2023

Cancers

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The aim of this paper was to characterise the isotopic composition of oral squamous cell carcinoma (OSCC) specimens of different areas of the oral cavity. Secondly, we assessed whether there was a correlation between clinical stages of OSCC and isotopic abundance. The IRMS procedure was performed on 124 samples derived from 31 patients with OSCC of 15 N and 13 C to assess the isotopic composition. From each individual, four samples from the tumour, two from the margins, and two samples of healthy oral mucous membranes were derived. The two samples from the tumour and two samples from the margin were additionally subjected to histopathological assessment. Then, statistical analysis was conducted. Tumour infiltration tissues of the lower lip were characterised by higher mean δ13C values compared to samples derived from cancers of the other oral cavity regions (−23.82 ± 1.21 vs. −22.67 ± 1.35); (p = 0.04). The mean percentage of nitrogen content in tumour tissues was statistically higher in patients with the most advanced cancers (11.89 ± 0.03%) versus the group of patients with II and III stage cancers (11.12 ± 0.02%); (p = 0.04). In patients at stage IV, the mean δ13C value in the cancer samples equalled −22.69 ± 1.42 and was lower than that in patients at less severe clinical stages (p = 0.04). Lower lip cancer tissues differed in the isotopic abundance of carbon in comparison with tissues derived from the group of combined samples from other locations. Values of δ13C observed in specimens derived from lower lip cancers were similar to those observed in healthy oral mucous membranes. Cancer tissues obtained from patients in the last stage of OSCC had a different isotopic composition in comparison with those obtained from earlier stages. To confirm these observations, further research on larger groups of patients is needed.

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Section: Discussionmentioning

confidence: 99%

“…A study of bladder cancer patients revealed that differences in the abundance of 15 N and 13 C can be used as potential markers to predict the recurrence of the disease. It was demonstrated that 13 C depletion in normal urothelium in patients with surgically treated bladder cancer is associated with shorter disease-free survival [5].…”

Section: Introductionmentioning

confidence: 99%

Oral Cavity Cancer Tissues Differ in Isotopic Composition Depending on Location and Staging

Bogusiak,

Kozakiewicz,

Puch

et al. 2023

Cancers

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Understanding natural isotopic variations in cultured cancer cells

Mantha,

Mahé,

Mahéo

et al. 2024

Rapid Comm Mass Spectrometry

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RationaleNatural variations in the abundance of the stable isotopes of nitrogen (δ15N) and carbon (δ13C) offer valuable insights into metabolic fluxes. In the wake of strong interest in cancer metabolism, recent research has revealed δ15N and δ13C variations in cancerous compared to non‐cancerous tissues and cell lines. However, our understanding of natural isotopic variations in cultured mammalian cells, particularly in relation to metabolism, remains limited. This study aims to start addressing this gap using metabolic modulations in cells cultured under controlled conditions.MethodsProstate cancer cells (PC3) were cultured in different conditions and their δ15N and δ13C were measured using isotope ratio mass spectrometry. Isotopic variations during successive cell culture passages were assessed and two widely used cell culture media (RPMI and DMEM) were compared. Metabolism was modulated through glutamine deprivation and hypoxia.ResultsSuccessive cell culture passages generally resulted in reproducible δ15N and δ13C values. The impact of culture medium composition on δ15N and δ13C of the cells highlights the importance of maintaining a consistent medium composition across conditions whenever possible. Glutamine deprivation and hypoxia induced a lower δ13C in bulk cell samples, with only the former affecting δ15N. Gaps between theory and experiments were bridged and the lessons learned throughout the process are provided.ConclusionsExposing cultured cancer cells to hypoxia allowed us to further investigate the relation between metabolic modulations and natural isotopic variations, while mitigating the confounding impact of changing culture medium composition. This study highlights the potential of natural δ13C variations for studying substrate fluxes and nutrient allocation in reproducible culture conditions. Considering cell yield and culture medium composition is pivotal to the success of this approach.

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The relationship between DNA methyltransferase 3B (DNMT3B) and miR 124-3pa expressions in bladder cancer tissues

Fawzy,

El Faiomy,

El Desoky

et al. 2023

Mol Biol Rep

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13C Natural Isotope Abundance in Urothelium as a New Marker in the Follow-Up of Patients with Bladder Cancer

Cited by 6 publications

References 32 publications

Oral Cavity Cancer Tissues Differ in Isotopic Composition Depending on Location and Staging

Oral Cavity Cancer Tissues Differ in Isotopic Composition Depending on Location and Staging

Understanding natural isotopic variations in cultured cancer cells

The relationship between DNA methyltransferase 3B (DNMT3B) and miR 124-3pa expressions in bladder cancer tissues

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