Hepatitis B virus genotypes in Uzbekistan and validity of two different systems for genotyping

Kato, Hideaki; Ruzibakiev, Ruslan; Yuldasheva, Nadira; Hegay, Tatyana; Kurbanov, Fuat; Achundjanov, Bakhodir; Туйчиев, Л. Н.; Usuda, Sadakazu; Ueda, Ryuzo; Mizokami, Masashi

doi:10.1002/jmv.10126

Cited by 44 publications

(35 citation statements)

References 19 publications

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“…In this regard, the commonest presentation in our study was accidental discovery as these blood donors were unaware that they were infected with HBV until they were found positive for HBsAg during routine screening after blood donation. A similar observation was reported in genotype D patients, where HBV infection was asymptomatic in approximately 75-85% of the subjects [31,32]. In contrast to these reports and ours, Thakur et al [33 ]found that patients infected with genotype D strains had more severe chronic liver disease.…”

Section: Discussioncontrasting

confidence: 54%

Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Habil¹,

Mahdi

Abdelwahab

et al. 2013

Intervirology

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Objectives: Hepatitis B virus (HBV) infection is a global health burden. In this regard, Egypt has an intermediate HBV seroprevalence. HBV is classified into ten different genotypes (A-J) with different geographic distributions. Genotype D is the most prevalent in the Middle East. Limited data are available about HBV genotyping among Egyptian blood donors, particularly in Upper Egypt. We examined the seroprevalence of HBV among 12,000 blood donors attending the blood transfusion services center in Minia Governorate, Upper Egypt. Methods: HBsAg and HBeAg were examined by ELISA while HBV-DNA was examined by PCR. HBV genotyping was conducted by restriction fragment length polymorphism. Results: HBsAg was detected in 237 donors (1.98%). The HBV-DNA of 50 donors with the highest HBsAg OD was examined for the HBV genotype. All 50 DNA-positive samples were of genotype D. 82% of the DNA-positive donors were males, coinciding with their representation in the cohort. ALT levels were normal in 88% of genotyped subjects, while 84% of them were HBeAg negative. Conclusion: Taken together, these data indicate that HBV genotype D is the predominant genotype among HBsAg-positive blood donors in Upper Egypt and was in >80% of the subjects associated with a negative HBeAg serostatus.

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Section: Discussioncontrasting

confidence: 54%

Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Habil¹,

Mahdi

Abdelwahab

et al. 2013

Intervirology

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“…HBV genotype D is reported to be related to acute self-limited hepatitis [9] . Furthermore, HBV genotype D has been found in the majority of asymptomatic carriers (84.2%) and it is not found in patients with liver cirrhosis and hepatocellular carcinoma [20] . These findings are in contrast with other studies [22,23] .…”

Section: Discussionmentioning

confidence: 98%

“…The viral genome controls antigen expression, leading to different genotypes and a disease spectr um after infection. Genotype D is dominant in the Mediterranean region [18] , the Middle East [19] and Central Asia [20] . However, the clinical outcomes of the individuals infected with HBV of genotype D are still controversial.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B virus genotypes in southwest Iran: Molecular, serological and clinical outcomes

Mojiri

Behzad-Behbahani

Saberifirozi

et al. 2008

WJG

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AIM:To investigate the associations of hepatitis B virus (HBV) genotype with HBeAg and anti-HBe status, alanine aminotransferase (ALT) levels and HBV-DNA detection in different groups of HBV-infected patients in southwest Iran. METHODS:A total of 89 HBsAg-positive serum samples were collected from the same number of patients. All sera were then investigated to determine HBV DNA and serological markers. For all the polymerase chain reaction (PCR)-positive samples, biochemical, histopathological assays and genotyping were also performed. RESULTS:Genotype D was the only type of HBV found in different clinical forms of acute and chronic infections. There was a high prevalence of HBeAg-negative HBVinfected patients with chronic hepatitis (52.7%). Out of 55 patients with chronic hepatitis, seven (12.7%) were diagnosed with cirrhosis. A significant association between the presence of anti-HBe antibody and an increase in ALT level, among either HBeAg-negative (P = 0.01) or HBeAg-positive (P = 0.026) patients, was demonstrated. No significant differences were observed between the clinical outcomes of HBeAg-positive and -negative individuals (P = 0.24). CONCLUSION:Genotype D has been recognized as the only type of HBV found in different clinical forms of HBV infections, including cirrhosis, among the residents of southwest Iran. Anti-HBe possibly plays a role in disease progression in some patients with chronic hepatitis, at least for a period of disease.

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“…These methods consist of PCR with genotype-specific primers (12,22,25) or PCR amplification of the pre-S region (15) or S region (19) followed by restriction fragment length polymorphism analysis. These methods offer a simple and rapid means of determining the HBV genotype; however, these methods may fail to type all isolates (27) and interpretation of results may be difficult, particularly in the case of mixed genotype infections (13). Several HBV genotyping methods are now commercially available, including the LiPA kit and an enzyme immunoassay (EIA) with monoclonal antibodies raised against genotype-specific epitopes in the pre-S2 region (HBV Genotype EIA; Institute of Immunology, Tokyo, Japan) (32,33).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the INNO-LiPA HBV Genotyping Assay for Determination of Hepatitis B Virus Genotype

2003

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Specific genotypes of hepatitis B virus (HBV) are increasingly recognized for their clinical significance and association with particular viral mutations. Although many HBV genotyping methods exist, there has been no standardized or commercially available method for direct molecular typing of the HBV genome. A newly available line probe assay (INNO-LiPA HBV Genotyping assay; Innogenetics N.V., Ghent, Belgium) that allows the identification of HBV genotypes A to G was assessed by comparison with pre-S1/pre-S2 sequence analysis of the isolates in 188 serum specimens. All seven genotypes were detected by the line probe assay (LiPA), and complete concordance between LiPA and sequence analysis was observed for 152 specimens (81%). LiPA was able to detect 19 mixed genotype infections not detected by amplicon sequencing, which for the most part were confirmed by cloning and sequencing of the pre-S1/pre-S2 amplicon. Four specimens had discrepant results between the two methods, and five specimens had indeterminate results by LiPA. The HBV DNA in four specimens was unable to be amplified by the nested INNO-LiPA HBV DR amplification primers; however, the HBV DNA in six specimens unable to be genotyped by sequencing was clearly genotyped by LiPA. The INNO-LiPA HBV Genotyping assay appears to be useful for the rapid genotyping of HBV, particularly for the sensitive detection of mixed genotype infections.It is estimated that more than 350 million individuals worldwide are chronically infected with hepatitis B virus (HBV), of which approximately 20 to 30% risk death from HBV-related liver failure or hepatocellular carcinoma (7, 18). In the past several years, the clinical significance of different HBV genotypes has become increasingly recognized in patients with both acute and chronic HBV infections. For example, genotype C has been more closely associated with a poor prognosis and a more aggressive clinical phenotype (8, 26), while genotype B has been associated with earlier HBeAg seroconversion (2), possibly leading to a lower prevalence of HBV-related cirrhosis. Core promoter and lamivudine resistance mutations were found to be more common in genotypes C and A, while precore stop mutations have been observed more frequently in genotypes B and D (6, 9, 30, 34, 35). Therefore, knowledge of the genotype infecting a patient may assist a physician in making clinical and therapeutic decisions.Although many HBV genotyping methods exist, there has been no standardized or commercially available method for direct molecular typing of the HBV genome. There are seven genotypes of HBV, based on nucleotide differences of 8% or greater along the entire length of the HBV genome or 4% or greater within the small S gene (HBsAg) (23). Recently, an assay based on the line probe assay (LiPA; INNO-LiPA HBV Genotyping assay, Innogenetics N.V., Ghent, Belgium) was released for research purposes. This method is based on the reverse hybridization principle, such that biotinylated amplicons hybridize to specific oligonucleotide probes that are immobi...

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Hepatitis B virus genotypes in Uzbekistan and validity of two different systems for genotyping

Cited by 44 publications

References 19 publications

Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Hepatitis B virus genotypes in southwest Iran: Molecular, serological and clinical outcomes

Evaluation of the INNO-LiPA HBV Genotyping Assay for Determination of Hepatitis B Virus Genotype

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