Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Habil, Febee E.; Mahdi, Wafaa K.M.; Abdelwahab, Sayed F.; Abdel‐Hamid, Mohamed

doi:10.1159/000353105

Cited by 16 publications

(8 citation statements)

References 38 publications

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“…HBV-genotype D2 was the most prevalent genotype in our patients (98.2%) and this is consistent with previous data obtained in Egypt [Habil et al, 2013;Zaky et al, 2010]. As regards, the serological and virological markers of HBV in the whole studied group, HBeAg-negative and HBeAb positive patients were 81.9% and 76.7%, respectively.…”

Section: Discussionsupporting

confidence: 92%

Influence of delta virus infection on the virologic status in Egyptian patients with chronic hepatitis B virus genotype D

Fouad

Abdo

Eldeen

et al. 2015

Journal of Medical Virology

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Hepatitis delta virus (HDV) usually have an unfavorable clinical outcome in chronic hepatitis B virus (HBV) patients. In Egypt, data about epidemiology, the spectrum of disease, and impact of HDV on HBV infection are rare. To assess the prevalence, clinical and virological characteristics of HDV infection among Egyptian patients with chronic HBV. Adult patients with Hepatitis B surface antigen (HBsAg)-positive were evaluated for the presence of HDV using anti HDV-IgG and HDV RNA by RT-PCR. Routine laboratory investigations, genotypes and subtypes for both HBV and HDV, abdominal sonography, and transient elastography (TE) were done. Liver biopsy was performed only in whenever indicated. One hundred and twenty-one treatment-naïve chronic HBV patients were included. Wild HBV genotype-D2 was found in 98.2% and 81.9% were HBeAg negative. Prevalence of HDV was 8.3% by anti-HDV IgG and 9.9% by RT-PCR. Wild HDV genotype-IIb was reported in 83.3%. HDV infection was more common in males, 90.9% of delta patients were HBeAg negative. Compared to the mono-infected HBV, concomitant HBV/HDV infection was not associated with more derangment in ALT nor advanced stage of fibrosis. 66.7% of HDV patients had significantly lower HBV-DNA level compared to the non-delta patients (P < 0.001). HDV is not uncommon in Egypt. HBV genotype-D was associated with HDV genotype-IIb. Delta infection was associated with negative HBeAg status, reduction of HBV replication, but neither influenced the clinical course nor increased significant liver damage risk.

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Section: Discussionsupporting

confidence: 92%

Influence of delta virus infection on the virologic status in Egyptian patients with chronic hepatitis B virus genotype D

Fouad

Abdo

Eldeen

et al. 2015

Journal of Medical Virology

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“…In the present work, fifteen (14.5%) donors were positive for HBeAg and comparable with other studies in Tanzania [ 21 ], Egypt [ 49 ], and Poland[ 47 ] but lower than reports from Ethiopia[ 50 ]and Benin [ 51 ]. Seropositivity for HBeAg, a marker of active viral replication and high infectiousness, is associated with higher risk for hepatocellular carcinoma; and it is significant regardless of serum level of ALT and status of liver cirrhosis[ 52 ].…”

Section: Discussionsupporting

confidence: 91%

“…In our study, ninety-eight (95.1%) of donors had ALT level below 1.5xULN, it is comparable to data reported in asymptomatic HBV carrier blood donors [ 15 , 47 , 49 ]. Those donors who had elevation of ALT level above 1.5xULN showed also higher viral load value, even if the relation was statistically insignificant ( p = 0.060).…”

Section: Discussionsupporting

confidence: 88%

Genotyping and sero-virological characterization of hepatitis B virus (HBV) in blood donors, Southern Ethiopia

et al. 2018

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Hepatitis B virus (HBV) prevalence is highest in Sub-Saharan Africa including Ethiopia. HBV genotypes have distinct geographic distributions and play a role in course of infection and treatment management. However, in Ethiopia there is paucity of information about distribution of HBV genotypes. This study was done to determine genotype, mutation and sero-virological profiles of HBV isolates in Southern Ethiopia. Cross-sectional, laboratory based study was conducted on 103HBsAg sero-positive samples from a total of 2,237 screened blood donors. HBV serological markers and biochemical assays were done. Serum viral load was measured using quantitative real-time PCR. Partial HBV S-gene was amplified with nested PCR and sequenced. Bioinformatics tools were utilized to determine genotypes, serotypes and mutations. Of 103 HBsAg reactive serum samples, 14.6% and 70.9% were sero-positive for HBeAg and HBeAb, respectively. Ninety-eight samples gave detectable viral load with a median of 3.46(2.62–4.82) log IU/ml. HBeAg sero-positive donors carried elevated levels of viral load. Eighty five isolates were successfully amplified, sequenced and genotyped into 58 (68.2%) genotype A (HBV/A) and 27 (31.8%) genotype D (HBV/D). HBV serotypes found were adw2 (74.1%), ayw2 (24.7%), and ayw3 (1.2%). In twenty-four (28.2%) samples mutations in the major hydrophilic region (MHR) were observed. Donors infected with HBV/A had higher viral load and more frequent MHR mutation than HBV/D infected donors. This study illustrated distribution of HBV genotype A and D among blood donors in southern Ethiopia. It also demonstrated occurrence HBV variants that may influence clinical aspects of HBV infection. The study contributes in narrowing the existing gap of HBV molecular study in Ethiopia.

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“…were HBeAb-positive. Nonetheless, HBeAg seropositivity for HBV-infected blood donors in our study was lower than those reported from Ethiopia [28], Egypt [29] and Tanzania [30]. HBeAg, a marker of active viral replication and higher infectiousness, is associated with higher risk for liver cirrhosis and hepatocellular carcinoma [31].…”

Section: Discussioncontrasting

confidence: 66%

Genotyping and sero-virological characterisation of hepatitis B virus-infected blood donors in Central Eritrea

Hamida¹,

Raja²,

Petros³

et al. 2021

Preprint

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Aim To determine serological markers and genotypes profiles of HBV isolates in Central Eritrea. Materials & methods A total of 191 HBsAg sero-positive samples were randomly selected for the study from 23,232 screened blood donors from 2015 to 2017. Enzyme-linked immunosorbent assay was used to perform HBV serological markers screening, while genotypes were determined using type-specific primer-based multiplex-nested PCR. Results The median age of infected blood donors was 28.9 ± 10.26 years. Of 191 HBsAg reactive serum samples, 77.5% (148/191) were sero-positive for HBcAb-total, among which 99.3% (147/148) and 0.7% (1/148) were sero-positive for HBsAg and HBsAb, respectively. Interestingly, among 147 HBcAb-total/HBsAg reactive samples, 16 (10.9%) and 131 (77.9%) were sero-positive for HBeAg and HBeAb, respectively. For genotyping, 73 HBV isolates were successfully amplified and genotyped, with 59 (80.8%) had a mono-genotype and 14 (19.2%) had a mixed-genotype infection. Among HBV isolates with mono-genotypes: 39 (53.4%) D; 10 (13.7%) E; 6 (8.2%) A and 4 (5.5%) C. While five mixed-genotypes comprised: 6 (8.2%) C/D; 3 (4.1%) C/D/E; 2 (2.7%) each with genotype A/D and D/E; and 1 (1.4%) genotype B/D. Conclusion HBV genotype D is the predominant genotype, either as a HBV mono- or mixed-genotype infection, among blood donors in Eritrea.

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Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Cited by 16 publications

References 38 publications

Influence of delta virus infection on the virologic status in Egyptian patients with chronic hepatitis B virus genotype D

Influence of delta virus infection on the virologic status in Egyptian patients with chronic hepatitis B virus genotype D

Genotyping and sero-virological characterization of hepatitis B virus (HBV) in blood donors, Southern Ethiopia

Genotyping and sero-virological characterisation of hepatitis B virus-infected blood donors in Central Eritrea

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