Hepatitis B virus genotypes in southwest Iran: Molecular, serological and clinical outcomes

Mojiri, Anahita; Behzad-Behbahani, Abbas; Saberifirozi, Mehdei; Ardabili, Maryam; Beheshti, Mahmood; Rahsaz, Marjan; Banihashemi, Mehrdad; Azarpira, Negar; Geramizadeh, Bita; Khadang, Baharak; Moaddeb, Afsaneh; Ghaedi, Mojgan; Heidari, Tahereh; Torab, A; Salah, Alireza; Amirzadeh, Saeid; Jowkar, Zahra; Mehrabani, Davood; Amini-Bavil-Olyaee, Samad; Dehyadegari, Mohammad Ali

doi:10.3748/wjg.14.1510

Cited by 24 publications

(17 citation statements)

References 22 publications

(25 reference statements)

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“…Previous findings indicated that there may be an association between the geographical distribution of HDV and HBV genotypes (38). As all reports from Iran showing that genotype D is the only detectable genotype in all clinical forms of HBV infection in this country (41, 42). It is expected that HDV genotype I was detected as predominant genotype in this study.…”

Section: Discussionsupporting

confidence: 57%

Frequency and Genotype of Hepatitis D Virus Infection in Patients Infected with HIV and Those Undergoing Hemodialysis

et al. 2013

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BackgroundHepatitis D virus (HDV) is a defective virus dependent on hepatitis B virus (HBV) for its replication. Due to HDV transmission routes, patients undergoing hemodialysis and those with HIV infection are at risk of acquiring HDV.ObjectivesThis study was aimed to determine the frequency and genotype of HDV infection among patients with HIV infection and those undergoing hemodialysis.Patients and Methods720 cases including 120 patients undergoing hemodialysis, and 600 patients with HIV infection were studied. All cases with positive results for HBsAg were evaluated for the presence of anti-HDV antibodies. Samples with Anti-HDV positive results were subjected to nested PCR for HDV-RNA confirmation, and sequenced for HDV genotype determination.ResultsHBsAg was found in 9 (7.5%) of 120 patients undergoing hemodialysis, and 9 (1.5%) of 600 patients with HIV infection. 3 (33.3%) of patients undergoing hemodialysis with positive results for HBsAg, and 5 (55.5%) of cases with HIV infection and positive results for HBsAg, had positive findings for anti-HDV which were then subjected to nested PCR. The amplification results confirmed that in 3 (37.5%) samples HDV-RNA was detected. Overall 2.5% of patients undergoing hemodialysis, and 0.8% of cases infected with HIV had positive results for anti-HDV and 1.7% and 0.2% of cases undergoing hemodialysis and patients infected with HIV had positive findings for HDV-RNA respectively. All of the HDV isolates were clustered in clade 1.ConclusionsThe survey showed that overall HDV frequency was not high in our high risk cases. Therefore, practitioners and health care managers should become aware of the risk of dual infection with HBV and HDV especially in high risk patients.

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Section: Discussionsupporting

confidence: 57%

Frequency and Genotype of Hepatitis D Virus Infection in Patients Infected with HIV and Those Undergoing Hemodialysis

et al. 2013

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“…In this regard, patients with detectable DNA and normal ALT levels are said to be in the immunotolerant phase [38]. In contrast to our study, 65.5% of the Iranian patients with chronic hepatitis B showed ALT levels that were higher than normal values in Iran [39]. Persistently normal or only slightly elevated ALT levels in blood were a characteristic finding in HBV-DNA-negative samples.…”

Section: Discussioncontrasting

confidence: 50%

Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Habil¹,

Mahdi

Abdelwahab

et al. 2013

Intervirology

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Objectives: Hepatitis B virus (HBV) infection is a global health burden. In this regard, Egypt has an intermediate HBV seroprevalence. HBV is classified into ten different genotypes (A-J) with different geographic distributions. Genotype D is the most prevalent in the Middle East. Limited data are available about HBV genotyping among Egyptian blood donors, particularly in Upper Egypt. We examined the seroprevalence of HBV among 12,000 blood donors attending the blood transfusion services center in Minia Governorate, Upper Egypt. Methods: HBsAg and HBeAg were examined by ELISA while HBV-DNA was examined by PCR. HBV genotyping was conducted by restriction fragment length polymorphism. Results: HBsAg was detected in 237 donors (1.98%). The HBV-DNA of 50 donors with the highest HBsAg OD was examined for the HBV genotype. All 50 DNA-positive samples were of genotype D. 82% of the DNA-positive donors were males, coinciding with their representation in the cohort. ALT levels were normal in 88% of genotyped subjects, while 84% of them were HBeAg negative. Conclusion: Taken together, these data indicate that HBV genotype D is the predominant genotype among HBsAg-positive blood donors in Upper Egypt and was in >80% of the subjects associated with a negative HBeAg serostatus.

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“…HBV and HCV genotyping was performed using polymerase chain reaction-restriction fragment length polymorphisms and genotype specific primers respectively, as previously described (15, 16). α-fetoprotein was detected by commercial quantities Enzyme linked Immunosorbent Assay kits (ConAg, Sweden) according to the manufactures' instructions.…”

Section: Methodsmentioning

confidence: 99%

Differentially Expressed Proteins in Chronic Active Hepatitis, Cirrhosis, and HCC Related to HCV Infection in Comparison With HBV Infection: A proteomics study

et al. 2013

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BackgroundHepatocellular carcinoma is a highly progressive cancer in the case of late diagnosis which is frequently associated with HBV and HCV viral infections.ObjectivesTo identify differentially expressed serum proteins among three main stages of HCV infection and healthy individuals, and their comparisons with sera from patients with the same stage of HBV infection.Patients and MethodsTwo-dimensional polyacrylamide gel electrophoresis combined with liquid chromatography-tandem mass spectrometry was performed on 47 sera from healthy volunteers, those with chronic active hepatitis, cirrhosis and HCC patients associated with HBV and HCV infections.ResultsAmong these, 62 spots were differentially expressed (≥ 1.5 fold; P < 0.05), of which 42 spots that corresponded to 15 proteins were identified by liquid chromatography-tandem mass spectrometry. CD5-like antigen (CD5L) was differentially expressed between cirrhosis and HCC patients with HCV infection. Leucine-rich α2-glycoprotein (LRG) and haptoglobin (HP) α2 isoforms differed in the HCC that was associated with either HCV or HBV infections.ConclusionsCD5L might be a useful biomarker for early diagnosis of HCC in HCV cirrhotic patients. LRG and HP α2 isoforms could be potential markers for distinguishing viral HCC. Our results also further support the presence of varying molecules involved in hepatocarcinogenesis in HBV when compared with HCV infection.

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Hepatitis B virus genotypes in southwest Iran: Molecular, serological and clinical outcomes

Cited by 24 publications

References 22 publications

Frequency and Genotype of Hepatitis D Virus Infection in Patients Infected with HIV and Those Undergoing Hemodialysis

Frequency and Genotype of Hepatitis D Virus Infection in Patients Infected with HIV and Those Undergoing Hemodialysis

Hepatitis B Virus Genotype D Predominates HBsAg-Positive Egyptian Blood Donors and Is Mainly Associated with a Negative HBeAg Serostatus

Differentially Expressed Proteins in Chronic Active Hepatitis, Cirrhosis, and HCC Related to HCV Infection in Comparison With HBV Infection: A proteomics study

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