Hepatit B Virüs (HBV)-DNA Seviyeleri ve Biyokimyasal Belirteçler Arasındaki İlişki

Abstract

In the present study, we aimed to evaluate the association between HBV-DNA levels and biochemical parameters, age, gender, and hepatitis B virus (HBV) serologic markers. Materials and Methods: A total of 124 HBsAg (+) serum samples of the patients with chronic hepatitis B, were included in the study. HBV-DNA level, HBV serological markers, alanine transaminase (ALT), aspartat transaminase, gama-glutamyl transferase (GGT), lipase, bilirubin, lactate dehydrogenase, and C-reactive protein levels in the samples were evaluated. HBV-DNA levels were quantitatively evaluated by real-time polymerase chain reaction (PCR) and serological markers were evaluated by ELISA. Results: With molecular testing, 116 samples were positive for HBV-DNA, of them 36.2% had an HBV-DNA level >2000 IU/mL. The number of samples with HBV-DNA levels >2000 IU/mL were higher in females than those of males. 44.4% of all HBeAg (+) patients with elevated ALT levels had an HBV-DNA level >2000 IU/ mL. All HBeAg (+) patients, 24.4% of HBeAg (-) patients, and 25.5% of anti-HBe (+) patients had elevated ALT levels. 99 of the 106 anti-HBe (+) carriers, of whom 27 had elevated ALT levels, were PCR positive. All HBeAg-positive samples were HBV-DNA positive. The rate of samples with elevated biochemical parameters except GGT levels were higher serian patients with HBV-DNA level >2000 IU/mL than in patients with HBV-DNA level <2000 IU/mL. A statistically significant relationship was detected only between lipase and HBV-DNA levels. Conclusion: It is clear that HBeAg seroconversion is not sufficiently definitive to determine the infectivity and it is crucial to evaluate HBV serological tests, HBV-DNA levels, transaminases levels besides the clinical picture of the patient in the diagnosis of the infection.