Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiol

Katsiadaki, Ioanna; Williams, Tim D.; Ball, Jonathan S.; Bean, Tim P.; Sanders, Matthew; Wu, Huifeng; Santos, Eduarda M.; Brown, Margaret; Baker, Paul R. S.; Ortega, Fernando; Falciani, Francesco; Craft, John A.; Tyler, Charles R.; Viant, Mark R.; Chipman, J.K.

doi:10.1016/j.aquatox.2009.07.005

Cited by 75 publications

(41 citation statements)

References 94 publications

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“…Therefore, some of these enzymatic parameters such as activity of acetylcholinesterase have been used as biomarkers for the heavy metal monitoring (Matozzo et al, 2005). With the development of system biology, metabolomics, one of the techniques of system biology, has been widely applied in drug toxicity studies, disease diagnosis, functional genomics and environmental sciences (Wu et al, 2005;Waters et al, 2001;Marchesi et al, 2007;Gavaghan et al, 2002;Griffin et al, 2004;Brindle et al, 2002;Bundy et al, 2004;Viant et al, 2006a,b;Katsiadaki et al, 2009). The application of proton nuclear magnetic resonance (NMR) spectroscopy combined with pattern recognition methods to detect the responses of low molecular weight metabolites (<1000 Da) to toxicants or environmental contaminants has been demonstrated in both terrestrial vertebrate and invertebrate systems relevant to environmental toxicology (Bundy et al, 2002;Griffin et al, 2000;Fiehn, 2002).…”

Section: Introductionmentioning

confidence: 99%

Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics

Zhang

Liu

You

et al. 2011

Marine Environmental Research

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Section: Introductionmentioning

confidence: 99%

Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics

Zhang

Liu

You

et al. 2011

Marine Environmental Research

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“…As described previously, all one-dimensional proton NMR spectra were converted to a format using custom-written ProMetab software in Matlab (version 7.0; The MathsWorks, Natick, MA, USA) to generate NMR spectral data (Purohit et al, 2004;Parsons et al, 2007;Katsiadaki et al, 2009). NMR spectral data were mean-centered before principal components analysis (PCA) using PLS Toolbox (version 4.0, Eigenvector Research, Manson, WA, USA).…”

Section: Metabolite Extraction Nmr Spectroscopy and Data Analysismentioning

confidence: 99%

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

Wu²,

Liu

et al. 2013

Chin. J. Ocean. Limnol.

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Ruditapes philippinarum , a clam that thrives in intertidal zones of various salinities, is a useful biomonitor to marine contaminants. We investigated the infl uence of dilution to 75% and 50% of normal seawater salinity (31.1) on the responses of the digestive gland of R . philippinarum to arsenic exposure (20 μg/L), using nuclear magnetic resonance (NMR)-based metabolomics. After acute arsenic exposure for 48 h, salinity-dependent differential metabolic responses were detected. In normal seawater, arsenic exposure increased the concentrations of branched-chain amino acids, and of threonine, proline, phosphocholine and adenosine, and it decreased the levels of alanine, hypotaurine, glucose, glycogen and ATP in the digestive glands. Differential changes in metabolic biomarkers observed at lower salinity (~23.3) included elevation of succinate, taurine and ATP, and depletion of branched-chain amino acids, threonine and glutamine. Unique effects of arsenic at the lowest salinity (~15.6) included down-regulation of glutamate, succinate and ADP, and up-regulation of phosphocholine. We conclude that salinity infl uences the metabolic responses of this clam to arsenic.

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“…SAM software was applied to fi nd signifi cant metabolic differences among control and heavy metal-exposed groups with appropriate false discovery rate (FDR) cutoffs. One-way ANOVA was conducted on the ratio of signifi cantly changed bins to identify signifi cant metabolites (at FDR<0.01; Katsiadaki et al, 2009). P =0.05 was considered signifi cant for the ANOVA on the metabolites between control and exposed samples.…”

Section: H Nmr Spectroscopy Data Processing and Multivariate Analysismentioning

confidence: 99%

Manila clam Venerupis philippinarum as a biomonitor to metal pollution

Wang

et al. 2013

Chin. J. Ocean. Limnol.

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The Manila clam Venerupis philippinarum is a good biomonitor/bioindicator to marine metal pollution and is frequently used in aquatic toxicology. Two dominant pedigrees (white and zebra) of clam are distributed in the Bohai Sea; however, little attention has been paid to potential biological differences between these two pedigrees. In this study, we tested the sensitivity of both pedigrees to marine metal (cadmium and zinc) pollution biomonitoring and marine environmental toxicology. Results demonstrate signifi cant biological differences in gills of white and zebra clams based on metabolic profi les and antioxidant enzyme activities. In addition, we found that hypotaurine, malonate and homarine were relatively high in white clam gills, while alanine, arginine, glutamate, succinate, 4-aminobutyrate, taurine and betaine were high in zebra clam gills. Zebra clam gills were also more sensitive to a mixture of Cd and Zn, as shown by antioxidant enzyme activities and metabolic profi les, but white clam gills could accumulate more Zn. Therefore, we suggest that the white pedigree can be used as a biomonitor to marine Zn pollution, whereas the zebra pedigree can be used for toxicology studies on Cd and Zn mixed pollution.

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Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiol

Cited by 75 publications

References 94 publications

Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics

Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

Manila clam Venerupis philippinarum as a biomonitor to metal pollution

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