Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics

Zhang, Linbao; Liu, Xiaoli; You, Liu; Zhou, Di; Wu, Huifeng; Li, Lianzhen; Zhao, Jianmin; Feng, Jianghua; Yu, Junbao

doi:10.1016/j.marenvres.2011.04.002

Cited by 99 publications

(54 citation statements)

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“…Consistent with previous studies (Hanana et al, 2014;Zhang et al, 2011), the NMR spectra of the gills from the clam R. philippinarum was dominated by organic osmolytes (Fig. 3).…”

Section: Discussionsupporting

confidence: 90%

“…2). These differences suggested disturbances in osmotic regulation associated with hypoxia, which have also been demonstrated when R. philippinarum is exposed to other stresses, such as salinity irregularity and environmental pollution Liu et al, 2014;Zhang et al, 2011;Liu et al, 2011). The alterations in the amount of these osmolytes reflect an innate response of the clams to balance their intracellular osmolarity with the local environment, as previously demonstrated for other marine organisms (Viant et al, 2003).…”

Section: Discussionsupporting

confidence: 58%

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Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics

Zhang

Wei

et al. 2017

Marine Pollution Bulletin

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Coastal hypoxia affects the survival, behavior, and reproduction of individual local marine organisms, and the abundance, biomass, and biodiversity of coastal ecosystems. In this study, we investigated the chronic effects of hypoxia on the metabolomics in the gills of Ruditapes (R.) philippinarum. The results indicated significant alterations in the metabolite profiles in the gills of the hypoxia-treated clams, in comparison with those maintained under normoxia. The levels of betaine, taurine, glycine, isoleucine, and alanine were significantly reduced, suggesting a disturbance of osmotic balance associated with hypoxia. Meanwhile, metabolites involved in energy metabolism, such as alanine and succinate, were also affected. Dramatic histopathological changes were observed in the gills and hepatopancreases of R. philippinarum grown in hypoxic waters, demonstrating tissue damages apparently caused by long-term exposure to hypoxia. Our findings suggest that hypoxia significantly affects the physiology of R. philippinarum, even at a sub-lethal level, and impedes health of the clams.

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“…Consistent with previous studies (Hanana et al, 2014;Zhang et al, 2011), the NMR spectra of the gills from the clam R. philippinarum was dominated by organic osmolytes (Fig. 3).…”

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 58%

Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics

Zhang

Wei

et al. 2017

Marine Pollution Bulletin

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“…Metabolomics is a well-developed system in biology and has been successfully employed in toxicology based on the metabolite fi ngerprinting for environmental pollutants (Bundy et al, 2004;Wu et al, 2010;Zhang et al, 2011). In this study, metal treatments (Cd, Zn, and Cd+Zn) induced signifi cant metabolic responses in both white and zebra clam gills.…”

Section: Differential Responses Of White and Zebra Clams To Metal Expmentioning

confidence: 83%

Manila clam Venerupis philippinarum as a biomonitor to metal pollution

Wang

et al. 2013

Chin. J. Ocean. Limnol.

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The Manila clam Venerupis philippinarum is a good biomonitor/bioindicator to marine metal pollution and is frequently used in aquatic toxicology. Two dominant pedigrees (white and zebra) of clam are distributed in the Bohai Sea; however, little attention has been paid to potential biological differences between these two pedigrees. In this study, we tested the sensitivity of both pedigrees to marine metal (cadmium and zinc) pollution biomonitoring and marine environmental toxicology. Results demonstrate signifi cant biological differences in gills of white and zebra clams based on metabolic profi les and antioxidant enzyme activities. In addition, we found that hypotaurine, malonate and homarine were relatively high in white clam gills, while alanine, arginine, glutamate, succinate, 4-aminobutyrate, taurine and betaine were high in zebra clam gills. Zebra clam gills were also more sensitive to a mixture of Cd and Zn, as shown by antioxidant enzyme activities and metabolic profi les, but white clam gills could accumulate more Zn. Therefore, we suggest that the white pedigree can be used as a biomonitor to marine Zn pollution, whereas the zebra pedigree can be used for toxicology studies on Cd and Zn mixed pollution.

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“…In a previous study, Cu exposure induced a significant ( Representative one-dimensional 500-MHz 1 H NMR spectra of the muscle tissue extracts from shrimps C. affinis from the reference site (S6183) and metal-polluted sites (S6283 and S5283, B and C). Keys: (1) leucine, (2) isoleucine, (3) valine, (4) ethanol, (5) lactate, (6) threonine, (7) alanine, (8) unknown 1 (1.54 ppm), (9) arginine, (10) glutamate, (11) glutamine, (12) methionine, (13) philippinarum (Zhang et al, 2011b), which was also observed in the shrimps from the metal-polluted site (S5283). Interestingly, Cu was the main metal contaminant in the shrimps from S5283 site (Table 1 and Fig.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

Zhao

et al. 2016

Marine Pollution Bulletin

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a b s t r a c t a r t i c l e i n f oMarine environment in the Laizhou Bay is potentially contaminated by metals from industrial discharges. In this study, metal concentrations in shrimps Crangon affinis indicated that two typical sites (S6283 and S5283) close to Longkou and Zhaoyuan cities along the Laizhou Bay have been contaminated by metals, including Cd, As, Cu, Ni, Co, and Mn. In particular, Cd and As were the main metal contaminants in S6283. In S5283, however, Cu was the most important metal contaminant. The metabolic responses in the shrimps indicated that the metal pollution in S6283 and S5283 induced disturbances in osmotic regulation and energy metabolism and reduced anaerobiosis, lipid metabolism, and muscle movement. However, alteration in the levels of dimethylglycine, dimethylamine, arginine, betaine, and glutamine indicated that the metal pollution in S5283 induced osmotic stress through different pathways compared to that in S6283. In addition, dimethylamine might be the biomarker of Cu in shrimp C. affinis.

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Metabolic responses in gills of Manila clam Ruditapes philippinarum exposed to copper using NMR-based metabolomics

Cited by 99 publications

References 50 publications

Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics

Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics

Manila clam Venerupis philippinarum as a biomonitor to metal pollution

Metabolic responses to metal pollution in shrimp Crangon affinis from the sites along the Laizhou Bay in the Bohai Sea

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