Manila clam Venerupis philippinarum as a biomonitor to metal pollution

Abstract

The Manila clam Venerupis philippinarum is a good biomonitor/bioindicator to marine metal pollution and is frequently used in aquatic toxicology. Two dominant pedigrees (white and zebra) of clam are distributed in the Bohai Sea; however, little attention has been paid to potential biological differences between these two pedigrees. In this study, we tested the sensitivity of both pedigrees to marine metal (cadmium and zinc) pollution biomonitoring and marine environmental toxicology. Results demonstrate signifi cant biological differences in gills of white and zebra clams based on metabolic profi les and antioxidant enzyme activities. In addition, we found that hypotaurine, malonate and homarine were relatively high in white clam gills, while alanine, arginine, glutamate, succinate, 4-aminobutyrate, taurine and betaine were high in zebra clam gills. Zebra clam gills were also more sensitive to a mixture of Cd and Zn, as shown by antioxidant enzyme activities and metabolic profi les, but white clam gills could accumulate more Zn. Therefore, we suggest that the white pedigree can be used as a biomonitor to marine Zn pollution, whereas the zebra pedigree can be used for toxicology studies on Cd and Zn mixed pollution.

Impact of metal pollution on shrimp Crangon affinis by NMR-based metabolomics

“…The altered AMP, phosphocholine and malonate implied the disturbance in energy metabolism caused by metal pollution in shrimps from the YRE site. In the previous study, both alanine and arginine were elevated in Cd-exposed clam Ruditapes philippinarum (Wu et al, 2013a), which is consistent with the alteration of alanine and arginine in shrimps from YRE site. Interestingly, Cd is the main metal contaminant in the shrimp from YRE site.…”

Metabolomic responses of sea cucumber Apostichopus japonicus to thermal stresses

“…Among these analytical techniques, 1 H NMR spectroscopy has received much attention for its advantages upon the simultaneous analysis of the low molecularweight metabolites in a short time Lindon et al, 2000;Picone et al, 2011). NMR-based metabolomics has been widely applied in multiple areas including biology (Nicholson et al, 1999), medicine (Dunn et al, 2011), toxicology (Kokushi et al, 2010;Li et al, 2006;Williams et al, 2009Williams et al, , 2011Williams et al, , 2014Wang, 2010, 2011;Wu et al, 2013bWu et al, , 2013c, immunology (Liu et al, , 2014 and nutriology (Collino et al, 2011). Regarding the temperature modulated marine organism, studies at the metabolic level have also been carried out in certain marine animals.…”

A metabolomic investigation of the effects of metal pollution in oysters Crassostrea hongkongensis

“…It might suggest that there is a reduced energy demand in oyster gills from BJ. Wu et al (2013) observed an increased level of glycogen in the gill tissues from Cd-exposed clams Ruditapes philippinarum. In this work, interestingly, the oyster gills from FG accumulated more Cd ($20 times of that from JZ) than did those in oyster samples from BJ ($12 times that from JZ, Table 1).…”