A metabolomic investigation of the effects of metal pollution in oysters Crassostrea hongkongensis

Ji, Chenglong; Wang, Qing; Wu, Huifeng; Tan, Qiao-Guo; Wang, Wen‐Xiong

doi:10.1016/j.marpolbul.2014.11.006

Cited by 29 publications

(6 citation statements)

References 33 publications

(42 reference statements)

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“…For every orthogonal projection to latent structure with discriminant analysis model, the corresponding loading plot was utilized to identify metabolites contributing to the clusters in the orthogonal projection to latent structure with discriminant analysis plot. Model coefficients incorporating the weight of the variables can enhance the interpretability of the model . The software MATLAB can generate loading plots according to the loading scores.…”

Section: Methodsmentioning

confidence: 99%

Chronic effects of copper in oysters Crassostrea hongkongensis under different exposure regimes as shown by NMR‐based metabolomics

Cao

Wang

2017

Enviro Toxic and Chemistry

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Traditional metal toxicity tests on organisms have mainly focused on continuous exposure at a fixed concentration. However, organisms are more likely exposed to pollutants intermittently in estuarine environments that are significantly impacted by anthropogenic activity. The present study examined whether different copper (Cu) exposure regimes at an equivalent dose can induce different metabolomics effects on the oysters. An estuarine oyster Crassostrea hongkongensis was exposed to Cu continuously or intermittently at an equal dose (time × concentration) for 6 wk. Continuous exposure regimes included 2 doses of 3.3 μg/L for 24 h and 20 μg/L for 24 h, with corresponding equal doses of 2 intermittent exposure regimes of 20 μg/L for 4 h and 120 μg/L for 4 h, respectively. Time-course measurements suggested that Cu bioaccumulation was comparable at equal low doses between the continuous regime (3.3 μg/L for 24 h) and the intermittent regime (20 μg/L for 4 h), but there was considerable difference for the high dose under different regimes. Nuclear magnetic resonance (NMR)-based metabolomics suggested that continuous and intermittent Cu exposures led to similar metabolite variation pattern in gills at an equal high dose, including decreased amino acids (e.g., aspartate, glycine, isoleucine, leucine, lysine, phenylalanine, threonine, and valine), lower energy-related compounds (e.g., adenosine triphosphate/adenosine diphosphate, acetate, citrate, and glycogen), and altered osmolytes (e.g., homarine and taurine). These biomarkers indicated disturbance of osmotic regulation and energy metabolism induced by Cu exposure regardless of regime. In addition, the 4-h intermittent Cu exposure resulted in slightly fewer adverse effects compared with the corresponding equal-dose continuous exposure. Oysters appeared to recover during the intervals of Cu exposure. The results indicated that metabolomic effects induced by Cu were more dose dependent than the Cu exposure regime. Environ Toxicol Chem 2017;36:2428-2435. © 2017 SETAC.

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Section: Methodsmentioning

confidence: 99%

Chronic effects of copper in oysters Crassostrea hongkongensis under different exposure regimes as shown by NMR‐based metabolomics

Cao

Wang

2017

Enviro Toxic and Chemistry

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“…Metabolomic analysis can characterize the alteration of biological samples, allowing the researcher to identify perturbed metabolic pathways and obtain vital information pertaining to biochemical networks (Viant et al, 2009;Wu et al, 2012). Therefore, metabolomics has been extensively applied in marine ecotoxicology due to its capability to globally characterize the metabolic responses in organisms stressed by environmental pollutants, which can elucidate the toxicological effects and select biomarkers (Cappello et al, 2013(Cappello et al, , 2015Ji et al, 2015;Yu et al, 2016;Liu et al, 2019). In our study, metabolomic analysis based on the NMR technique was employed to characterize the dose-dependent effects of DEHP in mussels at the metabolite level.…”

Section: Dose-dependent Effects Of Dehp At Metabolite Level In Musselsmentioning

confidence: 99%

Dose-Dependent Effects of Di-(2-Ethylhexyl) Phthalate (DEHP) in Mussel Mytilus galloprovincialis

Cao

Sun

et al. 2021

Front. Mar. Sci.

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Phthalic acid esters (PAEs) are environmental organic pollutants that are ubiquitous in the ocean, and di-(2-ethylhexyl) phthalate (DEHP) is the most widely used PAE. The environmental concentration of DEHP was reported to be up to 42.52 μg/L in seawater in the estuaries located in Jiaozhou Bay along the Yellow Sea. DEHP has been investigated with respect to its toxicity in marine organisms. However, evidence on the dose-dependent effects of DEHP remains contradictory and limited. We used marine mussel Mytilus galloprovincialis as the experimental animal to study the dose-dependent effects of various levels of exposure to DEHP (concentrations of 4, 12, 36, 108, and 324 μg/L). These effects and the underlying mechanisms were elucidated by the levels of antioxidant enzyme activity, gene expression, and metabolite. The results indicated that, at environmentally relevant concentrations (12 and 36 μg/L), DEHP induced significant hormetic effects. This was indicated by the U-shaped or inverted U-shaped responses of the gene expression levels related to stress response (CAT, GST, and MgGLYZ) and antioxidant enzyme activities (SOD and CAT). The metabolic profiles revealed that DEHP generally caused monophasic response in osmotic regulation (homarine) and biphasic response (hormesis) in energy metabolism (glucose, glycogen, and amino acids), respectively. These findings can aid in ecological risk assessment with respect to DEHP and the determination of hormetic dose responses.

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“…NMR-based metabolomics has been used to express bivalve responses to external disturbance, such as from pathogenic bacteria [ 17 ], estrogenic mixtures [ 18 ], hepatotoxic microcystins [ 19 ], ammonia nitrogen exposure [ 20 ], and high ρCO 2 levels [ 21 ]. Proton ( 1 H) NMR-based metabolomics has identified osmotic regulation and energy metabolism in C. hongkongensis to be disturbed following exposure to Cu, Zn, Pb, and other metals [ 22 , 23 ]. These diverse applications in bivalves suggest that NMR-based metabolomics is a powerful tool for assessing the metabolic response mechanism of stressed bivalves in general, and C. hongkongensis in particular.…”

Section: Introductionmentioning

confidence: 99%

Gender-Specific Metabolic Responses of Crassostrea hongkongensis to Infection with Vibrio harveyi and Lipopolysaccharide

Tuo

et al. 2022

Antioxidants

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Gender differences in the hemocyte immune response of Hong Kong oyster Crassostrea hongkongensis to Vibrio harveyi and lipopolysaccharide (LPS) infection exist. To determine if a gender difference also exists, we use a 1H NMR-based metabolomics method to investigate responses in C. hongkongensis hepatopancreas tissues to V. harveyi and LPS infection. Both infections induced pronounced gender- and immune-specific metabolic responses in hepatopancreas tissues. Responses are mainly presented in changes in substances involved in energy metabolism (decreased glucose, ATP, and AMP in males and increased ATP and AMP in LPS-infected females), oxidative stress (decreased glutathione in males and decreased tryptophan and phenylalanine and increased choline and proline in LPS-infected females), tricarboxylic acid (TCA) cycle (decreased α-ketoglutarate acid and increased fumarate in LPS-infected males, and decreased fumarate in LPS-infected females), and osmotic regulation (decreased trigonelline and increased taurine in V. harveyi-infected males and decreased betaine in V. harveyi-infected females). Results suggest that post-spawning-phase male oysters have a more significant energy metabolic response and greater ability to cope with oxidative stress than female oysters. We propose that the impact of oyster gender should be taken into consideration in the aftermath of oyster farming or oyster disease in natural seas.

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A metabolomic investigation of the effects of metal pollution in oysters Crassostrea hongkongensis

Cited by 29 publications

References 33 publications

Chronic effects of copper in oysters Crassostrea hongkongensis under different exposure regimes as shown by NMR‐based metabolomics

Chronic effects of copper in oysters Crassostrea hongkongensis under different exposure regimes as shown by NMR‐based metabolomics

Dose-Dependent Effects of Di-(2-Ethylhexyl) Phthalate (DEHP) in Mussel Mytilus galloprovincialis

Gender-Specific Metabolic Responses of Crassostrea hongkongensis to Infection with Vibrio harveyi and Lipopolysaccharide

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