Heparin nanogel-containing liposomes for intracellular RNase delivery

Abstract

Liposomes containing nanogels (liponanogels) were fabricated by sonicating heparin-Pluronic (HP) nanogels with pegylated lipids for ribonuclease (RNase) delivery. Liponanogels with an average diameter of 316 nm were obtained and their spherical morphology was elucidated by atomic force microscopy (AFM). Confocal laser scanning microscopy (CLSM) revealed the core-shell structure of the liponanogels using two different fluorescent dyes, showing that HP nanogels were localized in the core of the liposomes. Interestingly, the hybridization of these two systems remedies the drawbacks of each system while they hold their strengths called "WIN-WIN effect". When HP nanogels were used to encapsulate RNase in liponanogels, the loading of RNase was almost doubled as compared with the loading in liposomes without nanogels. Due to the presence of a lipid bilayer on the nanogels, the release of RNase was prolonged over 4 days whereas it was much faster (82% after 21 h) for bare HP nanogels. The cytotoxicity of the RNase-loaded liponanogels was much higher than that of free RNase because of the endocytic cellular uptake of the particles. We believe that these hybrid liponanogel systems can potentially be utilized for the hereditary diseases and targeted cancer therapy since they can efficiently load RNases and sustainly release in target cells.

Green processing of thermosensitive nanocurcumin-encapsulated chitosan hydrogel towards biomedical application

“…However, F127 shares some biological disadvantages, including low cell adhesion [19] and inability to be enzymatically degraded [20]. To overcome the drawbacks of F127 gels, F127 was grafted onto other biological polymers [21,22]. In this study, the thermosensitive grafted Chitosan-g-Pluronic (CP) copolymer was prepared and used as a dispersant for synthesizing Cur nanoparticles.…”

Self‐assembled poly(ethylene glycol) methyl ether‐grafted gelatin nanogels for efficient delivery of curcumin in cancer treatment

“…The significantly enhanced anticancer efficiency of CUR‐loaded Gel‐mPEG nanogels toward HeLa cells in comparison to free CUR in our study might be due to the better stability and improved solubility of poorly soluble CUR that was loaded in Gel‐mPEG nanogels. Moreover, the cellular internalization of CUR‐loaded nanogels can be readily by endocytosis, which is more effective than the passive diffusion of free CUR into the cells . Therefore, the therapeutic efficacy of CUR toward cancer cells can be enhanced by utilizing Gel‐mPEG nanogels as the delivery system.…”

“…Nanogel is a potential type of DDSs for the delivery of therapeutic agents having poor bioavailability . Among the various types of nanogels, biopolymer‐based nanogels, especially nanogels prepared from gelatin, have been paid much attention due to its biocompatibility and biodegradability .…”

The Engineering of Porous Silica and Hollow Silica Nanoparticles to Enhance Drug-loading Capacity

“…In this phase, the weight loss of PNS was approximately 30%, whereas that of HMSN was only 13%, indicating that a small amount of CTAB may have remained in the HMSN nanoparticles. In the blood circulation, it was suggested that the extravasation and renal clearance of nanoparticles of less than 10 nm in diameter are more expedited than those of larger nanoparticles (<200 nm), which are likely to be retained for a longer period [29,30]. Accordingly, the achieved size of synthesized HMSN products have the potential to allow the particles to bypass early clearance through kidney and vascular leakage.…”

“…Afterwards, dialysis with an MWCO 12-14 kDa membrane and freeze-drying took place to obtain the final solution. In the second step, a structure was formed by coating a thickness-controlled PNS layer on each sSiO2 using CTAB as the organic cotemplates [29]. The core@shell structure was denoted as sSiO2/SiO2/CTAB.…”