Hemolytic and genotoxic evaluation of organochalcogens in human blood cells in vitro

Santos, Danúbia Bonfanti; Schiar, Viviane Patrícia Pires; Paixão, Márcio W.; Meinerz, Daiane Francine; Nogueira, Cristina W.; Aschner, Michael; Rocha, João Batista Teixeira da; Barbosa, N.B.V.

doi:10.1016/j.tiv.2009.05.010

Cited by 37 publications

(29 citation statements)

References 73 publications

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“…Recently, our research group demonstrated that organoselenium and organotellurium present hemolytic and genotoxic effects in human blood cells (Santos et al, 2009a; Santos et al, 2009b; Caeran Bueno et al, 2013), which is in accordance with results published by other laboratories in experimental bacteria and rodent models (Degrandi et al, 2010). Similarly, organoselenides and tellurides can be toxic in different in vivo and in vitro models of animal pathologies (Maciel et al, 2000; Taylor, 1996; Stangherlin, Rocha & Nogueira, 2009; Moretto et al, 2007; Heimfarth et al, 2011; Heimfarth et al, 2012a; Heimfarth et al, 2012b; Comparsi et al, 2012).…”

Section: Introductionsupporting

confidence: 90%

“…Mice blood leukocytes were isolated and used in the comet test but no pre-incubation was carried out (Santos et al, 2009a; Santos et al, 2009b; Meinerz et al, 2011). …”

Section: Methodsmentioning

confidence: 99%

“…This test has been used to investigate the effect of many toxic agents on DNA (Collins & Harrington, 2002; Blasiak, Arabski & Krupa, 2004). The comet assay was performed under alkaline conditions according to the procedures described by Santos et al (2009a) and Santos et al (2009b). The slides obtained from white blood cells of treated mice were analyzed under blind conditions by at least two individuals.…”

Section: Methodsmentioning

confidence: 99%

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Differential genotoxicity of diphenyl diselenide (PhSe)₂and diphenyl ditelluride (PhTe)₂

Meinerz

Allebrandt

Mariano

et al. 2014

PeerJ

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Organoselenium compounds have been pointed out as therapeutic agents. In contrast, the potential therapeutic aspects of tellurides have not yet been demonstrated. The present study evaluated the comparative toxicological effects of diphenyl diselenide (PhSe)2 and diphenyl ditelluride (PhTe)2 in mice after in vivo administration. Genotoxicity (as determined by comet assay) and mutagenicicity were used as end-points of toxicity. Subcutaneous administration of high doses of (PhSe)2 or (PhTe)2 (500 µmol/kg) caused distinct genotoxicity in mice. (PhSe)2 significantly decreased the DNA damage index after 48 and 96 h of its injection (p < 0.05). In contrast, (PhTe) caused a significant increase in DNA damage (p < 0.05) after 48 and 96 h of intoxication. (PhSe)2 did not cause mutagenicity but (PhTe)2 increased the micronuclei frequency, indicating its mutagenic potential. The present study demonstrated that acute in vivo exposure to ditelluride caused genotoxicity in mice, which may be associated with pro-oxidant effects of diphenyl ditelluride. In addition, the use of this compound and possibly other related tellurides must be carefully controlled.

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Section: Introductionsupporting

confidence: 90%

“…Mice blood leukocytes were isolated and used in the comet test but no pre-incubation was carried out (Santos et al, 2009a; Santos et al, 2009b; Meinerz et al, 2011). …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Differential genotoxicity of diphenyl diselenide (PhSe)₂and diphenyl ditelluride (PhTe)₂

Meinerz

Allebrandt

Mariano

et al. 2014

PeerJ

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“…The blood samples were assayed by the comet assay under alkaline conditions, following procedures by Santos et al (2009). Peripheral leukocytes were incubated during 3 hours with the extract at three different concentrations, 0.02 g L -1 , 0.1 g L -1 and 0.2 g L -1 , the latter two being five and ten times more concentrated than normal when the plant is used.…”

Section: Comet Assay Samplesmentioning

confidence: 99%

In vivo and in vitro genotoxicity studies of aqueous extract of Xanthium spinosum

Güez

Waczuk

Pereira

et al. 2012

Braz. J. Pharm. Sci.

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The use of plants as a source of palliative or cure for pathological conditions is quite common worldwide. Xanthium spinosum (Asteraceae), popularly known in Brazil as 'espinho de carneiro', is an annual weed from South America, which has been used by empiric medicine to treat neoplasias. Owing to the extensive use of the above-mentioned plant and to the lack of reports about the real effects of its infusion, current study evaluated the genotoxic potential of its aqueous extract at concentrations 0.02 g L -1 , 0.1 g L -1 and 0.2 g L -1 by fish micronucleus test and by comet human leukocytes assay. The micronucleus test featured at least 50 cells with micronuclei to every 2,000 cells scored, as a mutagenic parameter. The comet assay was used as a parameter for assessing the level of cell damage and the damage index. Since no significant changes in strain cells exposed to the aqueous extract in the comet and micronucleus assays were reported, it seems that no genotoxicity evidence is extant at the concentrations and in the assays performed.Uniterms: Xanthium spinosum/pharmacognosy. Xanthium spinosum/aqueous extract/genotoxicity. Fish micronucleus assay. Comet assay.Em diversos lugares do mundo a utilização de plantas como fonte paliativa ou de cura para determinadas condições patológicas é bastante comum. No Brasil, essa prática não se torna diferente devido à ampla biodiversidade da fauna e flora presentes no País. Nesse contexto, surge a Xanthium spinosum (Asteraceae), conhecida popularmente como "espinho-de-carneiro", um arbusto anual introduzido na América do Sul, o qual tem sido utilizado empiricamente no tratamento de neoplasias. Sabendo do extensivo uso dessa planta em contrapartida com nenhum estudo reportando os reais efeitos de sua infusão, o objetivo do estudo foi avaliar a genotoxicidade do extrato aquoso nas concentrações de 0,02 g L -1 , 0,1 g L -1 e 0,2 g L -1 , através do ensaio do micronúcleo písceo e do ensaio cometa em leucócitos de sangue humano. O ensaio do micronúcleo tem como parâmetro mutagênico a presença de no mínimo 50 células com micronúcleo em uma contagem de 2.000 células por amostra, enquanto o ensaio cometa utiliza como parâmetro o nível de dano e o índice de dano. Os resultados mostram que não foi possível observar mudanças significativas nas células expostas ao extrato aquoso, em ambos os testes, o que sugere não existir evidência de genotoxicidade nas concentrações utilizadas no ensaio.Unitermos: Xanthium spinosum/farmacognosia. Xanthium spinosum/extrato aquoso/genotoxicidade. Ensaio do micronúcleo písceo. Ensaio cometa.

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“…32,33 These compounds generate free radicals and break DNA strand at elevated concentrations. 32,33 It is clear from the later discussion that only a narrow therapeutic window exists for the organoselenium compounds and the conventional drug delivery systems would not provide ideal pharmacokinetic profiles for these compounds. The ideal pharmacokinetic profile can be attained by reaching the therapeutic levels without exceeding the maximum endurable dose while maintaining the required concentration.…”

Section: Introductionmentioning

confidence: 99%

A Study on the Antioxidant Activity of Pyridylselenium Compounds and their Slow Release from Poly(acrylamide) Hydrogels

Dhau

Singh

et al. 2014

Phosphorus, Sulfur, and Silicon and the Related Elements

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The antioxidant activity of pyridylselenium compounds has been evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and nitric oxide (NO) scavenging methods. Pyridylselenium compounds have shown far superior (100-1000 times) antioxidant property than ebselen. The control release of bis(2-pyridyl) diselenide from poly(acrylamide) hydrogels has been studied in order to evaluate its release mechanism and diffusion coefficient. The later study also demonstrates that the pyridylselenium loading into the polymer matrix increases the magnitude and the rate of the radical scavenging activity of the poly(acrylamide) hydrogels.

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Hemolytic and genotoxic evaluation of organochalcogens in human blood cells in vitro

Cited by 37 publications

References 73 publications

Differential genotoxicity of diphenyl diselenide (PhSe)₂and diphenyl ditelluride (PhTe)₂

Differential genotoxicity of diphenyl diselenide (PhSe)₂and diphenyl ditelluride (PhTe)₂

In vivo and in vitro genotoxicity studies of aqueous extract of Xanthium spinosum

A Study on the Antioxidant Activity of Pyridylselenium Compounds and their Slow Release from Poly(acrylamide) Hydrogels

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Hemolytic and genotoxic evaluation of organochalcogens in human blood cells in vitro

Cited by 37 publications

References 73 publications

Differential genotoxicity of diphenyl diselenide (PhSe)2and diphenyl ditelluride (PhTe)2

Differential genotoxicity of diphenyl diselenide (PhSe)2and diphenyl ditelluride (PhTe)2

In vivo and in vitro genotoxicity studies of aqueous extract of Xanthium spinosum

A Study on the Antioxidant Activity of Pyridylselenium Compounds and their Slow Release from Poly(acrylamide) Hydrogels

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Product

Resources

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Differential genotoxicity of diphenyl diselenide (PhSe)₂and diphenyl ditelluride (PhTe)₂

Differential genotoxicity of diphenyl diselenide (PhSe)₂and diphenyl ditelluride (PhTe)₂