Hemin-mediated fluorometric determination of melatonin by high-performance liquid chromatography

Iizuka, Hideaki; Someya, Kousuke; Yajima, Takeaki

doi:10.1016/j.ics.2007.07.047

Cited by 4 publications

(4 citation statements)

References 4 publications

(5 reference statements)

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“…The analytical results were in good agreement with those obtained using a conventional method [9]. Iizuka et al performed fluorescence determination of melatonin by high-performance liquid chromatography (HPLC), and they obtained the structure of fluorescent products of melatonin [10]. Zhang et al classified phytoplankton with a fluorescent method, and the sinusoidal-amplitudemodulation (SAM) technique was used in this method.…”

Section: Introductionsupporting

confidence: 65%

Study on the Fluorescence Spectra Characteristics of Vinegar-Water Solutions

Wang

Han

2016

Journal of Spectroscopy

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Fluorescence spectra and polarization spectra of vinegar-water solutions with different concentration of CH3COOH have been studied. The characteristics and mechanism of fluorescence spectra are discussed, and polarization degree is calculated. Vinegar-water solutions are excited by ultraviolet (UV) light at 380 nm. The characteristic fluorescence peaks of the solution were identified at 445 nm and 470 nm. The type of emission fluorescence isπ⁎→n. With the increasing concentration of CH3COOH/pH value, the peak intensity is enhanced first, and then fluorescence quenching occurs. The polarization degree confirms the molecular orientation of different sample solutions. This research provides theoretical and experimental basis for the physical/chemical properties and quality of vinegar detection by florescence spectroscopy.

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Section: Introductionsupporting

confidence: 65%

Study on the Fluorescence Spectra Characteristics of Vinegar-Water Solutions

Wang

Han

2016

Journal of Spectroscopy

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“…[ 130 ] Some samples were prepared by heating with hemin and Na 2 H 3 CO 6 at 100°C for ¼ of an hour to yield a fluorescent product to facilitate the detection process. [ 131 ] Another derivatization procedure was performed by demethylation of MT into 5‐hydroxyindoles and then converting these into fluorescent compounds by treatment with benzylamine and potassium hexacyanoferrate(III). HPLC was operated in the reversed‐phase and the isocratic mobile phase; the spectrofluorometric analysis, at 480 nm, of the derivative obtained, showed a linear detector response in the range of 6 × 10 −7 –46.50 × 10 −7 μg/ml , with 1.05 × 10 −6 μg/ml .…”

Section: Detection Methodsmentioning

confidence: 99%

Up‐to‐date studies regarding the determination of melatonin by chromatographic methods

Alessa

Saber

Althakafy

2021

Archiv der Pharmazie

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Melatonin is an indolic compound that has been reported in the literature to exist in human-based samples, vertebrates, vegetables, fruits, and pharmaceutical products.Melatonin is considered a dietary supplement and can regulate circadian rhythms, although it has not been classified as a drug by the US Food and Drug Administration. Several analytical methods have been used for its detection. This study aimed to summarize the recent outcomes of the chromatographic methods such as electrophoretic methods, gas chromatography, and liquid chromatography, which have been used for the determination of melatonin in the last three decades, with the focus on gas chromatography and high-performance liquid chromatography methods.

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“…There are many methods reported for quantification of melatonin with different kind of matrix like plasma, saliva, pineal gland and biological samples by GC-MS Chemical ionisation (Fourtillan et al 1994), LC-MS/MS (Eriksson et al 2003;Cao et al 2006), GC-ECD (Greiner and Chan 1978), HPLC-FLD (Rizzo et al 2002;Vitale et al 1996;Iinuma et al 1999;Munoz et al 2009;Lizuka et al 2007;Itoh et al 1997), HPLC-Electrochemical detector (Chanut et al 1998) and HPLC chemiluminesence (Lu et al 2002). Melatonin in human milk was reported by RIA and the method was validated by GC-MS (Illnerova et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of melatonin in milk by LC-MS/MS

et al. 2013

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A rapid, reliable and sensitive LC-MS/MS method for the determination of melatonin in milk was developed and validated. Sample was extracted with dichloromethane and cleaned by passing through Chem Elut solid phase extraction cartridge. The solvent was evaporated to dryness, reconstituted with methanol and analysed by LC-MS/MS on Agilent zorbax Eclipse XDB C-18 rapid resolution analytical column. The analytical procedure was found to be accurate, precise and linear. The method accuracy was 92.2 % (range 90.06-94.58) and the mean precision was 1.55 % and the calibration was linear for 1 to 150 pg mL −1 (R 2 >0.99), the lowest limit of quantification (LLOQ) was 1 pg mL −1. 7-D Melatonin (7-DM) was used as an internal standard. This method was proved to be a promising method for the determination of melatonin for market milk and human milk samples.

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Hemin-mediated fluorometric determination of melatonin by high-performance liquid chromatography

Cited by 4 publications

References 4 publications

Study on the Fluorescence Spectra Characteristics of Vinegar-Water Solutions

Study on the Fluorescence Spectra Characteristics of Vinegar-Water Solutions

Up‐to‐date studies regarding the determination of melatonin by chromatographic methods

Quantitative determination of melatonin in milk by LC-MS/MS

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