Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae

Rodrigues, Robson Andrade; Silva, André Luiz do Nascimento; Siqueira, Mayara Schueroff; Pilarski, Fabiana; Leal, Cássia Rejane Brito; Kuibida, Karin Virgínia; Campos, Cristiane Meldau de; Fernandes, Carlos Eurico dos Santos

doi:10.1007/s10499-020-00566-5

Cited by 7 publications

(2 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the pyloric cecum, the folds are slightly higher and less branched, corroborating other studies (MARTIN; BLABER, 1984;RODRIGUES;CARGNIN-FERREIRA, 2017;SCADENG et al, 2020). These studies emphasize that the architecture of the intestinal mucosal folds of teleosts becomes increasingly complex according to growth, with a significant increase in muscular thickness of the tunic being also observed (POZZER, 2015;RODRIGUES;CARGNIN-FERREIRA, 2017).…”

Section: Discussionsupporting

confidence: 88%

Macro, micromorphological and histological aspects of the intestine pirarucu Arapaima gigas (SCHINZ, 1822) (Osteoglossiformes: Arapaimidae)

Pinto

Melo

Aquino

et al. 2022

AVB

View full text Add to dashboard Cite

Currently, the fish farming with Arapaima gigas has suffered from technological obstacles in the fields of reproduction, health and nutrition, making it necessary to know the morphology of its structures, so that it can advance in more technified research in scope of production in Rondônia state, as well as in aquaculture nutrition and health. Therefore, the aimed is to characterize the macro and microscopic morphology of posterior digestive system of A. gigas. The intestine of six specimens A. gigas in ideal slaughter size was analyzed. The analyzes were performed using light-sheet microscopy (LM) and scanning electron (SEM) techniques. The intestine basically showes similar histological characteristics in three analyzed portions (proximal, middle and distal). Same type of simple columnar epithelium with goblet cells was evidenced, with subtle variations in pattern of villi in each segment, and in number of goblet cells. In the rectum, the amount of goblet cells and evident longitudinal villi was expressive. Macroscopic anatomy and histology of the intestine A. gigas analyzed showes characteristics of adaptation to cultivation, according to their diet and habitat. The intestinal mucosa can divided into three distinct portions: proximal, middle and final intestine, in addition to the rectum and anus. In the pyloric cecum, the folds are slightly higher and poorly branched. The rectum, compared to the midgut, showed a higher occurrence of goblet cells in the mucosa. This increase in goblet cells observed in the posterior portion may related to the assimilation of ions and fluids that occur at this location.

show abstract

Section: Discussionsupporting

confidence: 88%

Macro, micromorphological and histological aspects of the intestine pirarucu Arapaima gigas (SCHINZ, 1822) (Osteoglossiformes: Arapaimidae)

Pinto

Melo

Aquino

et al. 2022

AVB

View full text Add to dashboard Cite

show abstract

“…While infectious agents are known to elicit disruption of blood homeostasis, multiple physical, chemical and biological stressors may often be combined (35)(36)(37)(38)(39), making it difficult to disentangle the link between infection-associated pathophysiological mechanisms of morbidity and mortality (40,41). Interestingly, loss of osmoregulatory functioning in the gills, gut and kidneys are thought to contribute to mortality in carp affected with another viral agent, the koi herpes virus (34), which none of our samples was positive for.…”

Section: Discussionmentioning

confidence: 99%

Carp Edema Virus Infection Is Associated With Severe Metabolic Disturbance in Fish

et al. 2021

View full text Add to dashboard Cite

Significant mortalities associated with emerging viral diseases are challenging the economy of common carp aquaculture. As such, there is an increased need to disentangle how infected fish cope with progressive disease pathology and lose the ability for homeostatic maintenance of key physiological parameters. A natural carp edema virus (CEV) infection outbreak at a carp fish farm provided an opportunity to examine diseased and healthy carp in the same storage pond, thereby contributing to our better understanding of CEV disease pathophysiology. The disease status of fish was determined using PCR-based virus identification combined with analysis of gill pathology. Compared with healthy control carp, the blood chemistry profile of CEV-infected fish revealed major disruptions in electrolyte and acid-base balance (i.e., hyponatraemia, hypochloraemia, hyperphosphatemia, elevated pH, base excess, and anion gap and decreased partial dissolved carbon dioxide). In addition, we recorded hyperproteinaemia, hyperalbuminaemia, hypotonic dehydration, endogenous hyperammonaemia, and decreased lactate along with increased creatinine, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase. Red blood cell associated hematology variables were also elevated. The multivariate pattern of responses for blood chemistry variables (driven by sodium, pH, partial dissolved carbon dioxide, ammonia, and albumin in the principal component analysis) clearly discriminated between CEV-infected and control carp. To conclude, we show that CEV infection in carp exerts complex adverse effects and results in severe metabolic disturbance due to the impaired gill respiratory and excretory functioning.

show abstract

Unraveling florfenicol's effects on splenic histology, erythrocytes, and hematology of healthy Oreochromis niloticus juveniles

Bardhan,

Abraham,

Das

et al. 2024

Applied Research

View full text Add to dashboard Cite

The study aimed to investigate the impact of administering the antibiotic florfenicol (FFC) through dietary means on Oreochromis niloticus. The experiment involved exposing the fish to FFC at doses 0 to 10 times the therapeutic dose of 15 mg/kg biomass per day for 10 consecutive days. The group receiving the therapeutic dose showed 100% survival on the 10th day of dosing. Histological analysis revealed dose‐dependent changes in the spleen, including an increase in sinusoidal space, splenic necrosis, white pulp proliferation, splenitis, and red pulp hemorrhages. Additionally, there was an exponential rise in leucocyte counts accompanied by a significant decrease in erythrocyte counts, indicating the development of anemia due to FFC. The observed splenic histological damage, along with increased erythrophagia, erythro‐membrane breakage, and poikilocytosis, highlighted the splenotoxic and haemotoxic effects of FFC at higher doses. Importantly, these toxic effects showed considerable reduction upon discontinuation of FFC dosing. The study emphasized the need to consider FFC‐induced splenotoxic and hemotoxic outcomes in O. niloticus during its application in aquaculture.This article is protected by copyright. All rights reserved.

show abstract

Hematological, biochemical, and histopathological responses in sorubim Pseudoplatystoma spp. experimentally infected with Lactococcus garvieae

Cited by 7 publications

References 49 publications

Macro, micromorphological and histological aspects of the intestine pirarucu Arapaima gigas (SCHINZ, 1822) (Osteoglossiformes: Arapaimidae)

Macro, micromorphological and histological aspects of the intestine pirarucu Arapaima gigas (SCHINZ, 1822) (Osteoglossiformes: Arapaimidae)

Carp Edema Virus Infection Is Associated With Severe Metabolic Disturbance in Fish

Unraveling florfenicol's effects on splenic histology, erythrocytes, and hematology of healthy Oreochromis niloticus juveniles

Contact Info

Product

Resources

About