Hematological and biochemical profile of BALB/c nude and C57BL/6 SCID female mice after ovarian xenograft

Abstract

Hematological and biochemical profile studies help to evaluate functional changes of animals used in experiments. The aim of this study was to determine the hematological and biochemical profile of immunosuppressed BALB/c nude and C57BL/6 SCID mice after bovine ovarian xenotransplantation. Therefore, a total of 74 female mice were divided into four groups: non-xenotransplanted animals, xenotransplanted animals, xenotransplanted animals treated with eCG and xenotransplanted animals treated with FSH + LH. After anesthesia, blood samples were collected and hematologic and biochemical values were evaluated. The results showed no significant differences (p ≤ 0.05) for hematological parameters between the control group and the treatment groups of both strains. However, considering the biochemical profile, it was observed an increase of AST concentrations (p ≤ 0.05) in both strains and a decrease of ALT concentrations (p ≤ 0.05) only in C57BL/6 SCID strain of the groups subjected to hormonal treatment compared with those non subjected. Additionally, the values of the renal enzymes, urea and creatinine, did not differ (p ≤ 0.05) between the groups. Our findings suggest that the xenotransplantation procedure as well as the hormonal dosages had no significant effect on the well-being of the animals considering the evaluated hematological and biochemical profile.

Hematological reference values and animal welfare parameters of BALB/C‐FMABC (Mus musculus) inoculated with Ehrlich tumor kept in the vivarium at ABC Medical School

“…The degree of anisocytosis should be confirmed by visual miscroscopic analysis. Even though RDW data showed statistically significant differences, no anisocytosis was found in the slides of the animals studied …”

“…The distribution of red blood cells (RDW) is calculated by the size of the red blood cells distributed up to 20% above the base of the automated histogram from the red blood cell count, determining the size of all evaluated red blood cells, thus showing the size variation between the erythrocytes in percentage …”

“…Laboratory tests are central to the evaluation of specific changes in the physiological and functional profile of an animal. In experimental procedures where pathological processes are induced, knowledge of normal reference values is indispensable, since some pathologies influence the metabolism and alter the test results, and use of the reference values allows evaluation of the degree of disease and effectiveness of treatments …”

Influence of graft size, histocompatibility,and cryopreservation on reproductive outcome following ovary transplantation in mice

“…Immunodeficient mice are frequently used as recipients for xenotransplantation [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]. Reports about transplantation of mouse ovaries into immunodeficient mice are rare.…”

“…Alternatively, adult immunodeficient mouse mutants could serve as universal recipients but are still rarely used for the transplantation of mouse ovaries [12,14]. In contrast, immunodeficient mice have frequently been used for xenotransplantation as recipients for ovary pieces from the rat [15], human [16][17][18][19][20][21], sheep [22,23], cat [24,25], cattle [26,27], wombat [28], elephant [29], dog [30,31], and lion [32].…”