Hematological reference values and animal welfare parameters of BALB/C‐FMABC (Mus musculus) inoculated with Ehrlich tumor kept in the vivarium at ABC Medical School

Abstract

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractBackground: Biochemical and hematological parameters are important tools for assessing the physiological profile of vital organs, and can be recorded to create reference values used for clinical diagnosis of diseases. Many research laboratories lack the means to establish their own set of reference parameters for use in their research, and while there are articles in the literature that discuss laboratory parameters for healthy BALB/c mice, few studies address the evaluation of these parameters in pathological situations, such as in mice inoculated with Ehrlich tumor.Method: BALB/c-FMABC mice previously inoculated with Ehrlich tumor were maintained under appropriate conditions. Blood samples were taken for analysis of hematological parameters using automated and semi-automated equipment to create a set of the animal welfare parameters for evaluation. Result:Results were obtained for all the hematological parameters for all groups analyzed. These showed: statistically significant differences between the initial and final tumor weight; comparable initial tumour volume and weight; an increase in leukocytes in the 7-day group with a characteristic predominance of lymphocytes and neutrophils; statistically significant changes in RDW in the 21-day group and in the welfare parameters in the 28-day group. Conclusion:The study successfully defined and established reference values for hematological and welfare parameters for all groups analyzed. K E Y W O R D S ehrlich tumor carcinoma, experimental animal model, reference value, welfare | 33 PESSINI Et al.

Development of a Quantitative FMRP Assay for Mouse Tissue Applications

“…Considering a physiological reduction in WBC number occurring between 3 weeks and 7-10 weeks of life, it is possible that some of the FMRP reduction may be accounted for by the WBC number difference. However, the hematological reference values for C57BL/6 mice at various ages are not uniform [46][47][48] and require a more detailed assessment for the normalization of FMRP on an individual basis. The study was limited by the volume of the collected blood in the youngest group.…”

Comparative transcriptome analyses of immune responses to LPS in peripheral blood mononuclear cells from the giant panda, human, mouse, and monkey

“…Thus, deeply exploring and understanding the immunity of giant pandas is important for disease prevention and protection. Compared with the limited accessibility of disease-relevant tissues, profiling blood samples is a minimally invasive way to assess and analyze immune health and function (Sen et al, 2017;Schmiedel et al, 2018;Pessini et al, 2020). Moreover, blood serves as a pipeline for circulating immune cells throughout the whole body and has the ability to reflect the signatures of diseases or immune dysfunction (Banchereau et al, 2016;Kotliarov et al, 2020).…”

Preliminary study regarding the effect of season on haematological parameters in Syrian Awassi rams

“…Many laboratories have no reference parameters and need to establish their own set for use. Reference values of haematological parameters for some species of animals were reported such as Italian Ariégeois, Bleu de Gascogne, Bracco italiano, Segugio italiano, and Briquet Griffon Vandeen hunting (Miglio et al 2020) and Ghana locally raised dogs (Opoku-Agyemang et al 2019), BALB/c mice in Brazil (Pessini et al 2020), Chinese water deer (Nie et al 2020), Brazilian geese (Benarrós et al 2020), Egyptian buffaloes (Abd Ellah et al 2019), Pakistani camels (Farooq et al 2011), Italian Piemontese and Blonde d'Aquitaine cattle (Tarantola et al 2020), Italian Heavy Draft horse (Miglio et al 2019) and Polish half-bred and Wielkopolska horses (Burlikowska et al 2015), and four Omani goat breeds (Al-Bulushi et al 2017).…”