Hemagglutinating Properties of CELO, an Oncogenic Avian Adenovirus

Abstract

Chicken-embryo-lethal-orphan (CELO) virus, Phelps strain, agglutinated erythrocytes at 37 C. The hemagglutinating activity, which is a function of complete and incomplete virus particles, was sensitive to heat but not to pH. The soluble components of the virus were similar in sedimentation characteristics to those obtained from human adenovirus type 1. The effects of chemical and physical agents on CELO hemagglutinin, CELO infectivity, and red-cell receptors suggested that the last were protein in nature and that cell-virus attachment was mediated by amino groups on the virion. The attachment of virus to red blood cells via the penton projection was demonstrated by electron microscopy.

Avian adenoviruses ‐ a review

“…Norrby (1971) noted fibres of 14 nm on FAV-1 virus, similar to members of Rosen's subgroup 2 which FAV-1 resembles in its haemagglutination properties. El Mishad et al (1975), however, demonstrated fibres measuring 17.5 nm on FAV-1 particles. Potter etal.…”

“…Later El Mishad et al (1975) demonstrated that FAV-1 (Phelps) failed to agglutinate dog, guinea-pig, hamster, human, mouse, rabbit and rhesus monkey erythrocytes. Rat cells were agglutinated and haemagglutination took place at 4°C and 37°C.…”

Adenoviruses of Vertebrate Animals

“…Avian Adenoviruses. El Mishad et al (1975) demonstrated that while rat erythrocytes were agglutinated at 4°C and 37°C, maximum titers were achieved between pH 6 and pH 9, with tempera tures between 20°C and 45°C. Anderson et al (1971) demonstrated that peaks of hemagglutinating activity were recorded at densities of 1.32 and 1.34 gm/ml in association with bands of complete and incomplete particles.…”

“…The mouse 1 (AL) strain has a fiber length of 29 nm (Wigand et al, 1977). However, Norrby (1971) found that the fiber length of Fl was 14 nm, and El Mishad et al (1975) demonstrated fibers of 17.5 nm on Fl particles. However, Norrby (1971) found that the fiber length of Fl was 14 nm, and El Mishad et al (1975) demonstrated fibers of 17.5 nm on Fl particles.…”

“…The bovine AAV agglutinates sheep, human, guinea pig, and mouse cells at 4°C and 22°C, and horse cells only at 4°C (Luchsinger et al, 1970). Avian adenoviruses are distinct from mammalian AAV types (Yates et al, 1973;El Mishad et al, 1975). Avian adenoviruses are distinct from mammalian AAV types (Yates et al, 1973;El Mishad et al, 1975).…”