Helium-assisted, solvent-free electro-activation of 3D printed conductive carbon-polylactide electrodes by pulsed laser ablation

Głowacki, Maciej J.; Cieślik, Mateusz; Sawczak, Mirosław; Koterwa, Adrian; Kaczmarzyk, Iwona; Jendrzejewski, Rafał; Szynkiewicz, Lukasz; Ossowski, Tadeusz; Bogdanowicz, Robert; Niedziałkowski, Paweł; Ryl, Jacek

doi:10.1016/j.apsusc.2021.149788

Cited by 23 publications

(18 citation statements)

References 81 publications

(88 reference statements)

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“…Reagentless approaches including the use of laser and plasma sources are rapid but require a proper instrument to provide the treatment. − Chemical procedures and their combination with electrochemical treatment seem to be more accessible to generate enhanced results. , Greener protocols have also been proposed considering the elimination of toxic solvents …”

Section: Surface Treatmentsmentioning

confidence: 99%

Electrochemical (Bio)Sensors Enabled by Fused Deposition Modeling-Based 3D Printing: A Guide to Selecting Designs, Printing Parameters, and Post-Treatment Protocols

et al. 2022

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The 3D printing (or additive manufacturing, AM) technology is capable to provide a quick and easy production of objects with freedom of design, reducing waste generation. Among the AM techniques, fused deposition modeling (FDM) has been highlighted due to its affordability, scalability, and possibility of processing an extensive range of materials (thermoplastics, composites, biobased materials, etc.). The possibility of obtaining electrochemical cells, arrays, pieces, and more recently, electrodes, exactly according to the demand, in varied shapes and sizes, and employing the desired materials has made from 3D printing technology an indispensable tool in electroanalysis. In this regard, the obtention of an FDM 3D printer has great advantages for electroanalytical laboratories, and its use is relatively simple. Some care has to be taken to aid the user to take advantage of the great potential of this technology, avoiding problems such as solution leakages, very common in 3D printed cells, providing well-sealed objects, with high quality. In this sense, herein, we present a complete protocol regarding the use of FDM 3D printers for the fabrication of complete electrochemical systems, including (bio)sensors, and how to improve the quality of the obtained systems. A guide from the initial printing stages, regarding the design and structure obtention, to the final application, including the improvement of obtained 3D printed electrodes for different purposes, is provided here. Thus, this protocol can provide great perspectives and alternatives for 3D printing in electroanalysis and aid the user to understand and solve several problems with the use of this technology in this field.

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Section: Surface Treatmentsmentioning

confidence: 99%

Electrochemical (Bio)Sensors Enabled by Fused Deposition Modeling-Based 3D Printing: A Guide to Selecting Designs, Printing Parameters, and Post-Treatment Protocols

et al. 2022

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“…An increase in the amount of C=O bonds was found due to benzoic acid electrografting (FS-1), tracked with O 1 s signals at approximately 532.2 and 531.1 eV, corresponding to carbonyls and hydroxyls, respectively [54] , [55] . A simultaneous increase in the C 1 s C=O peak intensity can be tracked in the Supplementary Information file, Fig.…”

Section: Resultsmentioning

confidence: 99%

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

Brodowski¹,

Pierpaoli²,

Janik³

et al. 2022

Sensors and Actuators B: Chemical

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“…The biofunctionalisation procedure is presented in detail in section 2.2, while its schematic representation is shown in An increase in the amount of C=O bonds was found due to benzoic acid electrografting (FS-1), tracked with O 1s signals at approximately 532.2 and 531.1 eV, corresponding to carbonyls and hydroxyls, respectively (Glowacki et al, 2021;Ryl et al, 2019b). A simultaneous increase in the C 1s…”

Section: Effect Of Bdd Electrode Biofunctionalisation and Its Respons...mentioning

confidence: 99%

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: multivariate data analysis of multisine impedimetric response

Brodowski

Pierpaoli

Janik

et al. 2022

Preprint

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the cells through the binding of spike protein to the host cell surface-expressing angiotensin-converting enzyme 2 (ACE2) or by endocytosis mediated by extracellular matrix metalloproteinase inducer (CD147). We present extended statistical studies of the multisine dynamic electrochemical impedance spectroscopy (DEIS) revealing interactions between Spike RBD and cellular receptors ACE2 and CD147, and a reference anti-RBD antibody (IgG2B) based on a functionalised boron-doped diamond (BDD) electrode. The DEIS was supported by a multivariate data analysis of a SARS-CoV-2 Spike RBD assay and cross-correlated with the atomic-level information revealed by molecular dynamics simulations. Our approach allowed us to study and detect subtle changes in the electrical properties responsible for the susceptibility of cellular receptors to SARS-CoV-2, revealing their interactions. Changes in electrical homogeneity in the function of the RBD concentration led to the conclusion that the ACE2 receptor delivers the most homogeneous surface, delivered by the high electrostatic potential of the relevant docking regions. For higher RBD concentrations, the differences in electrical homogeneity between electrodes with different receptors vanish. Collectively, this study reveals interdependent virus entry paths involving separately ACE2, CD147, and spike protein, provided by a developed biosensing platform for the rapid screening of cellular interactions (i.e. testing various mutations of SARS-CoV-2 or screening of therapeutic drugs).

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Helium-assisted, solvent-free electro-activation of 3D printed conductive carbon-polylactide electrodes by pulsed laser ablation

Cited by 23 publications

References 81 publications

Electrochemical (Bio)Sensors Enabled by Fused Deposition Modeling-Based 3D Printing: A Guide to Selecting Designs, Printing Parameters, and Post-Treatment Protocols

Electrochemical (Bio)Sensors Enabled by Fused Deposition Modeling-Based 3D Printing: A Guide to Selecting Designs, Printing Parameters, and Post-Treatment Protocols

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: multivariate data analysis of multisine impedimetric response

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