Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

Brodowski, Mateusz; Pierpaoli, Mattia; Janik, Monika; Kowalski, Marcin; Ficek, Mateusz; Ślepski, Paweł; Trzaskowski, Bartosz; Swain, Greg M.; Ryl, Jacek; Bogdanowicz, Robert

doi:10.1016/j.snb.2022.132427

Cited by 11 publications

(4 citation statements)

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“…0.22 V vs Ag|AgCl (3 M KCl)), in the frequency range 10 kHz to 0.1 Hz, 10 points per decade and peak-to-peak amplitude of 10 mV. The impedimetric results were fitted using dedicated software built in the LabView environment and based on the Nelder–Mead algorithm and previously used in similar studies. , Selection of the electric equivalent circuit (see Figure A, inset) is discussed in the SI file, Section 3. Cyclic voltammetry (CV) was performed in the range from −0.30 to 0.65 V with a scan rate of 100 mV s –1 in triplicate.…”

Section: Methodsmentioning

confidence: 99%

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Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

et al. 2023

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In this work, we study the electrodes with a periodic matrix of gold particles pattered by titanium dimples and modified by 3-mercaptopropionic acid (MPA) followed by CD147 receptor grafting for specific impedimetric detection of SARS-CoV-2 viral spike proteins. The synergistic DFT and MM/MD modeling revealed that MPA adsorption geometries on the Au−Ti surface have preferential and stronger binding patterns through the carboxyl bond inducing an enhanced surface coverage with CD147. Control of bonding at the surface is essential for oriented receptor assembling and boosted sensitivity. The complex Au−Ti electrode texture along with optimized MPA concentration is a crucial parameter, enabling to reach the detection limit of ca. 3 ng mL −1 . Scanning electrochemical microscopy imaging and quantum molecular modeling were performed to understand the electrochemical performance and specific assembly of MPA displaying a free stereo orientation and not disturbed by direct interactions with closely adjacent receptors. This significantly limits nonspecific interceptor reactions, strongly decreasing the detection of receptor-binding domain proteins by saturation of binding groups. This method has been demonstrated for detecting the SARS virus but can generally be applied to a variety of protein−antigen systems. Moreover, the raster of the pattern can be tuned using various anodizing processes at the titania surfaces.

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Section: Methodsmentioning

confidence: 99%

“…Scanning electrochemical microscopy (SECM) measurements were carried out utilizing a commercially available system (Sensolytics GmbH), details previously reported. 28 The tests were conducted using 5 mM K 4 [Fe(CN) 6 ] dissolved in PBS (pH 7.4). All experiments were performed in feedback mode.…”

Section: Methodsmentioning

confidence: 99%

Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

et al. 2023

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“…AXL is a putative cell receptor for Zika virus ( Nowakowski et al, 2016 ), and implicated in mediating cell entry via the endosome pathway by SRAR-CoV-2 ( Bohan et al, 2021 ). CD147 is a membrane glycoprotein known to be involved in infection by eukaryotic, prokaryotic and viral pathogens ( Fenizia et al, 2021 ), and may also bind to SARS-2S and mediate the cell entry of SARS-CoV-2 through endocytosis ( Brodowski et al, 2022 ; Cavezzi et al, 2022 ; Kalejaiye et al, 2022 ), although infection mediated by CD147 is most likely secondary because CD147 is highly expressed in neural tissues but not in respiratory tract ( Qiao et al, 2020 ). Both genes appear weakly expressed in lungs based on tissue-specific gene expression data in The Human Protein Atlas ( Uhlén et al, 2015 ).…”

Section: Identification Of Host Receptor and Cofactorsmentioning

confidence: 99%

Identification of host receptors for viral entry and beyond: a perspective from the spike of SARS-CoV-2

Xia¹

2023

Front. Microbiol.

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Identification of the interaction between the host membrane receptor and viral receptor-binding domain (RBD) represents a crucial step for understanding viral pathophysiology and for developing drugs against pathogenic viruses. While all membrane receptors and carbohydrate chains could potentially be used as receptors for viruses, prioritized searches focus typically on membrane receptors that are known to have been used by the relatives of the pathogenic virus, e.g., ACE2 used as a receptor for SARS-CoV is a prioritized candidate receptor for SARS-CoV-2. An ideal receptor protein from a viral perspective is one that is highly expressed in epithelial cell surface of mammalian respiratory or digestive tracts, strongly conserved in evolution so many mammalian species can serve as potential hosts, and functionally important so that its expression cannot be readily downregulated by the host in response to the infection. Experimental confirmation of host receptors includes (1) infection studies with cell cultures/tissues/organs with or without candidate receptor expression, (2) experimental determination of protein structure of the complex between the putative viral RDB and the candidate host receptor, and (3) experiments with mutant candidate receptor or homologues of the candidate receptor in other species. Successful identification of the host receptor opens the door for mechanism-based development of candidate drugs and vaccines and facilitates the inference of what other animal species are vulnerable to the viral pathogen. I illustrate these approaches with research on identification of the receptor and co-factors for SARS-CoV-2.

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“…Severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) has caused a global health crisis with significant clinical morbidity and mortality since its emergence (Zhang et al, 2023). Although angiotensin‐converting enzyme 2 (ACE2) is recognized as the primary functional receptor for SARS‐CoV‐2 viral entry, a variety of cell surface and extracellular matrix (ECM) proteins have demonstrated binding affinity for the SARS‐CoV‐2 receptor binding domain (RBD) (Brodowski et al, 2022; Iacobucci et al, 2022; Kadam et al, 2021; Norris et al, 2023). The degree to which these molecules may modulate infectivity, however, remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Molecular interactions between perlecan LG3 and the SARS‐CoV‐2 spike protein receptor binding domain

Gressett,

Hossen,

Talkington

et al. 2023

Protein Science

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Severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) has caused a global health crisis with significant clinical morbidity and mortality. While angiotensin‐converting enzyme 2 (ACE2) is the primary receptor for viral entry, other cell surface and extracellular matrix proteins may also bind to the viral receptor binding domain (RBD) within the SARS‐CoV‐2 spike protein. Recent studies have implicated heparan sulfate proteoglycans, specifically perlecan LG3, in facilitating SARS‐CoV‐2 binding to ACE2. However, the role of perlecan LG3 in SARS‐CoV‐2 pathophysiology is not well understood. In this study, we investigated the binding interactions between the SARS‐CoV‐2 spike protein RBD and perlecan LG3 through molecular modeling simulations and surface plasmon resonance (SPR) experiments. Our results indicate stable binding between LG3 and SARS‐CoV‐2 spike protein RBD, which may potentially enhance RBD‐ACE2 interactions. These findings shed light on the role of perlecan LG3 in SARS‐CoV‐2 infection and provide insight into SARS‐CoV‐2 pathophysiology and potential therapeutic strategy for COVID‐19.

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Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

Cited by 11 publications

References 75 publications

Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces

Identification of host receptors for viral entry and beyond: a perspective from the spike of SARS-CoV-2

Molecular interactions between perlecan LG3 and the SARS‐CoV‐2 spike protein receptor binding domain

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