Helicobacter pylori BabA–SabA Key Roles in the Adherence Phase: The Synergic Mechanism for Successful Colonization and Disease Development

Doohan, Dalla; Rezkitha, Yudith Annisa Ayu; Waskito, Langgeng Agung; Yamaoka, Yoshio; Miftahussurur, Muhammad

doi:10.3390/toxins13070485

Cited by 43 publications

(43 citation statements)

References 69 publications

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“…The adhesion of H. pylori to lymphocytes may be mediated by the interaction between the bacterium’s own molecules and molecules on the cell surface. H. pylori strain 26695, that was used in this research, contains LacdiNAc domain-binding adhesins (LabA), lipoproteins A and B (AlpA/AlpB), sialic acid-binding adhesin (SabA), blood group antigen-binding adhesin (BabA), and lipopolysaccharide (LPS), which are recognized by mucin 5AC (MUC5AC), laminin, Lex sialic acid (Le x ), Lewis b antigen (Le b ), and Toll like-receptor 4 (TLR4), respectively, which are all present in PBMCs [ 3 , 24 , 25 , 26 , 27 ]. On the other hand, for the first time it is reported that the CagA oncoprotein of H. pylori enters the cytoplasm of PBMCs.…”

Section: Discussionmentioning

confidence: 99%

In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis

Atrisco-Morales

Ramı́rez

Castañón-Sánchez³

et al. 2022

IJMS

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Helicobacter pylori promotes the secretion of cytokines that regulate inflammation and carcinogenesis. Immune cells secrete cytokines into the extracellular medium or packaged in exosomes. The objective of this study was to analyze the profile of soluble and exosomal cytokines that were secreted by human peripheral blood mononuclear cells (PBMCs) that were infected with H. pylori and to build a network of interaction between cytokines and cellular proteins. PBMCs were obtained by density gradient centrifugation and infected with H. pylori for 24 h. The infection was verified by immunofluorescence and Western blot for CagA. The exosomes were obtained from culture supernatant by ultracentrifugation and characterized by transmission electron microscopy, particle size analysis, and Western blot for CD9 and CD81. Cytokines were quantified using a multiplex immunoassay in the culture supernatant, intact exosomes, and lysed exosomes. H. pylori adheres to lymphocytes and translocates CagA. In PBMCs, H. pylori induces an increase in the soluble and exosomal IL-1β, IL-6, TNF-α, IL-10, IL-17A, IL-21, and IL-22. The protein–protein interaction (PPI) network shows that soluble and exosomal cytokines interact with proteins that participate in signaling pathways such as NF-κB, MAPK, PI3K-Akt, Jak-STAT, FoxO, and mTOR, that are related to carcinogenesis; moreover, TNF-α had the highest number of interactions. Cytokine-loaded exosomes represent another means of intercellular communication that is activated by H. pylori to stimulate inflammation, carcinogenesis, or cancer progression. Cytokine-loaded exosomes are likely to be associated with extragastrointestinal diseases of inflammatory origin.

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Section: Discussionmentioning

confidence: 99%

In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis

Atrisco-Morales

Ramı́rez

Castañón-Sánchez³

et al. 2022

IJMS

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“…SabA recognizes Sialyl-Lewis x and sialyl-Lewis antigens expressed on the gastric epithelium [ 115 ]. Both BabA and SabA enables H. pylori to adhere to the gastric mucosa and subsequent colonization [ 116 ].…”

Section: Virulence and Pathogenesis Mechanismsmentioning

confidence: 99%

Helicobacter pylori: an up-to-date overview on the virulence and pathogenesis mechanisms

Sharndama

Elibe

2022

Braz J Microbiol

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Helicobacter pylori is an organism associated with ulcer disease and gastric cancer. The latter is one of the most prevalent malignancies and currently the fourth major cause of cancer-related deaths globally. The pathogen infects about 50% of the world population, and currently, no treatment ensures its total elimination. There has been an increase in our understanding of the pathophysiology and pathogenesis mechanisms of H. pylori over the years. H. pylori can induce several genetic alterations, express numerous virulence factors, and trigger diverse adaptive mechanisms during its adherence and colonization. For successful colonization and infection establishment, several effector proteins/toxins are released by the organism. Evidence is also available reporting spiral to coccoid transition as a unique tactic H. pylori uses to survive in the host’s gastrointestinal tract (GIT). Thus, the virulence and pathogenicity of H. pylori are under the control of complex interplay between the virulence factors, host, and environmental factors. Expounding the role of the various virulence factors in H. pylori pathogenesis and clinical outcomes is crucial for vaccine development and in providing and developing a more effective therapeutic intervention. Here we critically reflect on H. pylori infection and delineate what is currently known about the virulence and pathogenesis mechanisms of H. pylori .

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“…The major activators for NF- κ B and AP-1 by H. pylori are CagA and LPS [ 46 , 47 ]. Adhesion molecules of H. pylori , antigen binding adhesion A (BabA), sialic acid binding adherence A (SabA), or outer membrane protein Q (HopQ), allow attachment of the bacteria to gastric epithelial cells [ 48 ]. LPS binds to TLR4, a member of signal transduction proteins, to induce the expression of transcriptional factors including NF- κ B and AP-1 mostly in macrophages and gastric epithelial cells [ 49 ].…”

Section: H Pylori and Immune Systemmentioning

confidence: 99%

The Effects of Vitamins and Micronutrients on Helicobacter pylori Pathogenicity, Survival, and Eradication: A Crosstalk between Micronutrients and Immune System

Nabavi-Rad

Azizi

Jamshidizadeh

et al. 2022

Journal of Immunology Research

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Helicobacter pylori as a class I carcinogen is correlated with a variety of severe gastroduodenal diseases; therefore, H. pylori eradication has become a priority to prevent gastric carcinogenesis. However, due to the emergence and spread of multidrug and single drug resistance mechanisms in H. pylori, as well as serious side effects of currently used antibiotic interventions, achieving successful H. pylori eradication has become exceedingly difficult. Recent studies expressed the intention of seeking novel strategies to improve H. pylori management and reduce the risk of H. pylori-associated intestinal and extragastrointestinal disorders. For which, vitamin supplementation has been demonstrated in many studies to have a tight interaction with H. pylori infection, either directly through the regulation of the host inflammatory pathways or indirectly by promoting the host immune response. On the other hand, H. pylori infection is reported to result in micronutrient malabsorption or deficiency. Furthermore, serum levels of particular micronutrients, especially vitamin D, are inversely correlated to the risk of H. pylori infection and eradication failure. Accordingly, vitamin supplementation might increase the efficiency of H. pylori eradication and reduce the risk of drug-related adverse effects. Therefore, this review aims at highlighting the regulatory role of micronutrients in H. pylori-induced host immune response and their potential capacity, as intrinsic antioxidants, for reducing oxidative stress and inflammation. We also discuss the uncovered mechanisms underlying the molecular and serological interactions between micronutrients and H. pylori infection to present a perspective for innovative in vitro investigations, as well as novel clinical implications.

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Helicobacter pylori BabA–SabA Key Roles in the Adherence Phase: The Synergic Mechanism for Successful Colonization and Disease Development

Cited by 43 publications

References 69 publications

In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis

In Peripheral Blood Mononuclear Cells Helicobacter pylori Induces the Secretion of Soluble and Exosomal Cytokines Related to Carcinogenesis

Helicobacter pylori: an up-to-date overview on the virulence and pathogenesis mechanisms

The Effects of Vitamins and Micronutrients on Helicobacter pylori Pathogenicity, Survival, and Eradication: A Crosstalk between Micronutrients and Immune System

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